Core-Shell Silver/Polymeric Nanoparticles-Based Combinatorial Therapy against Breast Cancer In-vitro

Elbaz, Nancy M.; Ziko, Laila; Siam, Rania; Mamdouh, Wael

doi:10.1038/srep30729

Cited by 65 publications

(32 citation statements)

References 42 publications

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“…However, NPs were well-dispersed in the composite (as shown in the TEM micrographs) in spite of their low zeta potential. This is may be attributed to the stabilizing effect of the large molecular weight of PVP to AgNPs through steric stabilization as mentioned by Elbaz et al [48]. The AgNPs produced by reduction via the NaBH 4 are stabilized by the capping effect of PVP.…”

Section: Particle Size Distribution and Zeta Potentialmentioning

confidence: 84%

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

et al. 2017

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Polyvinylpyrrolidone (PVP) is employed in several potential applications, relying of its special chemical and physical properties in addition to its low toxicity and biocompatibility. The aim of this work is to prepare polyvinylpyrrolidone-silver (PVP-Ag) nanocomposite with high inhibiting effect on the microbial growth and low cytotoxicity. In situ prepared small stable spherical silver nanoparticles, with narrow range particle size distribution, were obtained by easy, economical and rapid chemical reduction method. Silver ions were reduced to silver nanoparticles using low amount of sodium borohydride (NaBH4) as a strong reducing agent. PVP-Ag nanocomposite was prepared using PVP as a stabilizing and capping agent. Formation of the spherical silver nanoparticles with mean particle size 5 nm was confirmed by ultraviolet-visible spectroscopy, high resolution transmission electron microscopy, and dynamic light scattering. The inhibiting growth effect of the nanocomposite toward Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa), and yeast fungus (Candida albicans) were studied. The cytotoxicity of the nanocomposite against BJ1 normal skin fibroblast cell line was tested. Results of this work presented perfect antimicrobial activity of the PVP-Ag nanocomposite towards bacteria and fungi with low cytotoxicity, which may lead to promising applications in skin wound healing.

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Section: Particle Size Distribution and Zeta Potentialmentioning

confidence: 84%

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

et al. 2017

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“…It has been reported that cellular uptake of free DOX was mediated by a passive diffusion, while the internalization of DOX-loaded nanoparticles involved an endocytosis mechanism [47]. The endocytosis leading to increase accumulation of drug into cells compared to passive diffusion [48]. The drug loading mode (non-covalent complexation or covalent conjugation) was also reported as a crucial factor which influences therapeutic efficiency, so that the non-covalently loaded DOX to nanoparticles exhibited significantly higher cytotoxicity than covalently conjugated DOX [47].…”

Section: Discussionmentioning

confidence: 99%

Multifunctional fluorescent titania nanoparticles: green preparation and applications as antibacterial and cancer theranostic agents

Masoudi

Mashreghi

Goharshadi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Theranostic nanoparticles have attracted considerable attention in recently revolutionized medicine. Since the last decade, there has been a growing attempt to design various theranostic nanoparticles but difficulties still exist in the fabrication of their biocompatible one. Herein, fluorescent titania nanoparticles (FTN) were fabricated using a one-step green method. This FTN had ultra-high doxorubicin hydrochloride (DOX) loading capacity (encapsulation efficiency 95.50% and loading content 38.20%) that release the loaded drug in response to acidic pH. In vitro cytotoxicity experiments on human osteosarcoma (SaOs-2) and breast cancer (MCF-7) cell lines revealed superior anticancer efficacy (lowered the IC concentration by 3- and 5.5-fold for SaOs-2 and MCF-7 cells, respectively) and also better imaging for intracellular tracking of DOX/FTN relative to free DOX. Furthermore, the prepared nanoparticles showed efficient antibacterial activity against both gram-negative (Escherichia coli ATCC 25922) and gram-positive (Staphylococcus aureus ATCC 25923) bacteria. In this study, we have developed novel theranostic titania nanoparticles with inherent fluorescence property for cancer imaging and therapy.

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“…It is concluded that the AgNPs can be used as an effective drug delivery system for targeting cancer because of its pHsensitive properties. AgNPs in BNCs system exhibit the active drug release in the acidic environment of cancer cells which increases bioavailability and therapeutic concentration of drug to cancer cells compared to healthy cells [41,42].…”

Section: The Surface Zeta Potentialmentioning

confidence: 99%

Antibacterial and Anticancer Potential of Silver Nanoparticles Synthesized Using Gallic Acid in Bentonite/Starch Bio-Nanocomposites

Thapliyal

Chandra

2018

Int J App Pharm

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Objective: To optimize and synthesize eco-friendly and low-cost silver nanoparticles (AgNPs) by using gallic acid (GA) reducing agent in bentonite/starch bio-nanocomposites (BNCs) for oral use and to evaluate its antibacterial and anticancer efficacy.Methods: An artificial neural network (ANN) model was employed for the optimization and evaluate the effect of the formulation variables on the entrapment efficiency (EE) of AgNPs. The synthesized AgNPs in BNCs were characterized using UV-vis spectroscopy, energy dispersive X-ray spectroscopy (EDXA), dynamic light scattering (DLS), scanning electron microscopy (SEM), zeta potential and fourier transform infrared spectroscopy (FTIR). Elemental ion analysis was carried out using inductively coupled plasma mass spectrometry (ICP-MS). Drug release study was carried out. The antimicrobial efficacy determined by agar well diffusion method. In vitro anticancer efficacy of AgNPs in breast cancer cell line (MCF-7) by MTT assay was performed.Results: The formation of AgNPs was confirmed by UV-vis absorbance peak shown at 412 nm. XRD spectrum has indicated the face-centered cubic structure of the synthesized AgNPs. SEM and DLS measurements showed spherical nanoparticles with a mean size of 68.06±0.2 nm. The negative surface zeta potential with-32±0.25 mV has indicated colloidal stability of nanoparticles. FTIR spectra confirmed no interaction observed between drug and excipients. AgNPs showed significant EE with 80±0.25%. The synthesized AgNPs in BNCs is a potential candidate for inhibiting the growth of pathogenic bacteria and showed significant cytotoxicity against MCF-7 cancer cell line with IC50 of 160±0.014μg/ml.Conclusion: The present research confirms that the green synthesized AgNPs in BNCs can be a promising antibacterial and anticancer agent regarding stability, low cost and easy preparation.

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Core-Shell Silver/Polymeric Nanoparticles-Based Combinatorial Therapy against Breast Cancer In-vitro

Cited by 65 publications

References 42 publications

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

Multifunctional fluorescent titania nanoparticles: green preparation and applications as antibacterial and cancer theranostic agents

Antibacterial and Anticancer Potential of Silver Nanoparticles Synthesized Using Gallic Acid in Bentonite/Starch Bio-Nanocomposites

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