Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

Abstract

Polyvinylpyrrolidone (PVP) is employed in several potential applications, relying of its special chemical and physical properties in addition to its low toxicity and biocompatibility. The aim of this work is to prepare polyvinylpyrrolidone-silver (PVP-Ag) nanocomposite with high inhibiting effect on the microbial growth and low cytotoxicity. In situ prepared small stable spherical silver nanoparticles, with narrow range particle size distribution, were obtained by easy, economical and rapid chemical reduction method. Silver ions were reduced to silver nanoparticles using low amount of sodium borohydride (NaBH4) as a strong reducing agent. PVP-Ag nanocomposite was prepared using PVP as a stabilizing and capping agent. Formation of the spherical silver nanoparticles with mean particle size 5 nm was confirmed by ultraviolet-visible spectroscopy, high resolution transmission electron microscopy, and dynamic light scattering. The inhibiting growth effect of the nanocomposite toward Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa), and yeast fungus (Candida albicans) were studied. The cytotoxicity of the nanocomposite against BJ1 normal skin fibroblast cell line was tested. Results of this work presented perfect antimicrobial activity of the PVP-Ag nanocomposite towards bacteria and fungi with low cytotoxicity, which may lead to promising applications in skin wound healing.

PVP-stabilized tungsten oxide nanoparticles (WO3) nanoparticles cause hemolysis of human erythrocytes in a dose-dependent manner

“…2(a)) contains two broad maxima at 12.1 • and 19.9 • 2θ which are characteristic for pure PVP. These data are in a good agreement with previously reported results [14,15]. The sharp peaks are corresponding to ITO substrate signal.…”

A Library of Potential Nanoparticle Contrast Agents for X-Ray Fluorescence Tomography Bioimaging

“…The results agree well with the earlier reports using the same synthesis methods [20]. Nevertheless, there is a diffraction peak around 20° for both the products, which is attributed to PVP that forms a capping layer on the formed NPs [36] or the residual solvent ethylene glycol from the washing process.…”

The destruction of Escherichia coli adhered to pipe surfaces in a model drinking water distribution system via various antibiofilm agents

“…Results obtained indicated that 100 mg/L of silver ions in drinking water can bring about severe toxicity for humans. Likewise, the exposure to silver ions is considered safe for humans as far as a dose of 0.1 mg/L of soluble silver is not exceeded in drinking water (El Hotaby, Sherif, Hemdan, Khalil, & Khalil, 2017; Hemdan & Elliethy, 2016). Interestingly, a report implied that the majority of bacterial strains could not acquire resistance to Ag‏ ions, which made it necessary to find out a more efficient alternative disinfectant that does not cause toxicity to humans (Hemdan et al., 2015; Silver, 2003).…”