Core-shell lipid polymer nanoparticles for combined chemo and gene therapy of childhood head and neck cancers

Abstract

Pediatric head and neck cancers account for overall 12% of all pediatric cancers. Despite recent advances in therapeutic modalities, children with tumor metastasis have poor prognosis. Therefore, there is an unmet need for new and effective treatment modalities for pediatric head and neck cancers. The present study describes a simple and efficient method for fabrication of cationic lipid‑polymer hybrid nanoparticles (CLPNs) for co‑delivery of cisplatin (CDDP) and DNA (CDDP/DNA CLPNs) for the therapy of childhood head and neck cancers. CDDP/DNA CLPNs were prepared by the modified double emulsion solvent evaporation method with self‑assembly. CDDP‑loaded CLPNs (CDDP CLPNs), CDDP-loaded polymeric nanoparticles (PNPs) (CDDP PNPs), and DNA‑loaded Lipofectamine® 2000 (DNA LIPO) were also prepared for comparison. The results illustrated that the concentration of the cationic lipid has influence on the characteristics of CLPNs. In vitro anticancer effect, in vitro transfection efficiency, in vivo antitumor and gene delivery efficacy of CDDP/DNA CLPNs have advantages over other formulations tested. In conclusion, outstanding delivery ability of CLPNs for both CDDP and DNA could combine the therapeutic efficiency of both drug and gene for the treatment of pediatric rhabdomyosarcoma (RMS).

Lipid-chitosan hybrid nanoparticles for controlled delivery of cisplatin

“…Hence the cytotoxic effect of cisplatin is time and concentration dependent. Optimum activity after 48 hours is in agreement with the previous studies (Zhang et al., 2017). It is also important that after 48 hours, the cisplatin loaded lipid-polymer hybrid nanoparticles showed a 20–30% increased cytotoxicity at same concentration as compared to 24 hours and up to 50% of cell death at the lowest concentration.…”

Combination Therapy of Metastatic Castration-Recurrent Prostate Cancer: Hyaluronic Acid Decorated, Cabazitaxel-Prodrug and Orlistat Co-Loaded Nano-System

“…One of the major concerns for nanoparticles is the electrostatic interaction between the vectors and the plasma composition such as proteins, which will increase the size of the nanoparticles. 51 During the stability studies, there were no remarkable changes in the particle size in the presence of plasma and during 3 months of storage at 4°C, which would prove the ability of the system when administration in vivo and enhance the targeting proportion of the vector to the target organ, and thus would be favorable to improve the therapeutic effects.…”

New Organosilicon Composite Based on Borosiloxane and Zinc Oxide Nanoparticles Inhibits Bacterial Growth, but Does Not Have a Toxic Effect on the Development of Animal Eukaryotic Cells

“…The presence of such a number of duplicating mechanisms suggests that the cell perceives 8-oxoguanine as an extremely serious threat that must be quickly eliminated [55]. Studies (Figure 5b) show that borosiloxane did not affect the formation of 8-oxoguanine in DNA in vitro, which significantly distinguishes it from other materials [56][57][58]. It was shown that when zinc oxide nanoparticles appear in BS, the rate of 8-oxoguanine formation in DNA significantly increases.…”