Core–shell fluorinated methacrylate nanoparticles with Rhodamine-B for confocal microscopy and fluorescence correlation spectroscopy applications

Jarzębski, Maciej; Zhang, Yingze; Śliwa, Tomasz; Mazuryk, Jarosław; Deptuła, Tobiasz; Kucińska, Małgorzata; Murias, Marek; Buitenhuis, Johan; Gapiński, Jacek; Patkowski, A.

doi:10.1016/j.jfluchem.2016.01.014

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…The fluorinated fluorescent polymer NPs from 1H,1H-heptafluoro-n-butyl methacrylate monomers with covalently bonded Rhodamine-B (RBITC) are useful and versatile systems for confocal laser scanning microscopy and fluorescence correlation spectroscopy. This has been confirmed by former studies of the structure and dynamics in colloidal systems [16].…”

Section: Introductionsupporting

confidence: 80%

“…Their high carrier capacity is combined with efficient carrier protection sustaining its activity [14]. On the other hand, there are various NP systems with the same basic material used in the whole particle; however, the layer-by-layer production-this process is also called "seed-growth" [15,16]-allows for the incorporation of different agents into chosen layers, e.g., fluorophores.…”

Section: Introductionmentioning

confidence: 99%

“…When using fluorescent CS nanoparticles in microscopic studies, great attention is paid to obtaining the right particle size and stability of the dye contained in them. One of the solutions is the preparation of fluorescent core-transparent shell particles [16,21]. In the case of polymerization during the seed-growth process, a small part of the fluorescent agent might be incorporated not only in the center of the particle, but also in the next core layer [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability

et al. 2020

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Fluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effectively protected the dye against bleaching. HFBMA nanoparticle (NP) stability was confirmed after seven years of storage. Only slight differences were found in the polydispersity index (pdi) from 0.002 to 0.010. Particle size measurements were carried out using dynamic light scattering (DLS), nanoparticle tracking (NTA), and fluorescence correlation spectroscopy (FCS). The hydrodynamic diameter evaluated by different methods were in good agreement, respectively: 184–550 nm, 218–579 nm, and 236–508 nm. Particle and core morphology was estimated by using scanning and transmission electron microscopy (SEM and TEM). The ability to recognize particles in 3D as a reference sample in biological media has been confirmed by epifluorescence optical microscopy, confocal laser scanning microscopy, and super-resolution confocal microscopy (STED).

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Section: Introductionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability

et al. 2020

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“…Their unique physical, optical, and electronic features may provide high activity and unusual properties through nanotechnology means [1]. It seems clear that in recent years nanoparticles are popular not only with respect to their potential bioapplications (i.e., drug delivery, biosensors, and bioscaffolds) but also due to the intense development of nanomaterials themselves resulting in numerous model systems as the probes to investigate and/or detect different physical phenomena [2][3][4]. None of the less polymeric nanoparticles have been widely used for biological applications including drug delivery since they must be responsive to external stimuli such as temperature and pH [5].…”

Section: Introductionmentioning

confidence: 99%

pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells

et al. 2021

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The biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties and vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-based cargo system that would effectively deliver a biological agent to human skeletal myogenic cells (SkMCs); among other aspects, a downregulating apoptotic pathway potentially responsible for poor regeneration of myocardium. We confirmed the size of the pNiPAM based spheres at around 100 nm and the nanomeric shape of nanoparticles (NP) obtained. We confirmed that 33 °C is the adequate temperature for phase transition. We performed the dynamics of cargo release. A small amount of examined protein was detected at 10 min after reaching LCTS (lower critical solution temperature). The presented results of the test with BSA (bovine serum albumin) and doxorubicin loaded into nanoparticles showed a similar release profile for both substances. SkMCs incubated with NP loaded with antiapoptotic agent, BCB (Bax channel blocker), significantly diminished cell apoptosis (p < 0.01). Moreover, the lowest apoptotic level was detected in SkMCs treated with camptothecin and simultaneously incubated with pNiPAMs loaded with BCB. Application of nanoparticles loaded with BCB or subjected to BCB alone did not, however, diminish the amount of apparently necrotic cells.

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“…Colloidal suspensions of fluorinated particles stand to find uses in coatings applications [ 1 – 6 ], as optical devices [ 7 , 8 ], low-refractive-index particles and media for microscopy [ 9 – 12 ], and as model colloidal systems for a wide range of fundamental studies [ 13 – 20 ]. Such fluorinated particles are usually synthesized via conventional emulsion polymerization [ 21 – 23 ] or mini- [ 24 – 26 ] and microemulsion [ 27 ] polymerization.…”

Section: Introductionmentioning

confidence: 99%

Semi-batch synthesis of colloidal spheres with fluorinated cores and varying grafts of poly(ethylene glycol)

et al. 2017

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Fluorinated spheres with grafted poly(ethylene glycol) (PEG) have been synthesized using a semi-batch emulsion polymerization in which the initiator is fed slowly to the reaction. In this way, PEG-grafted colloidal spheres can be fabricated with varying PEG chain length, different cores and varying degrees of crosslinking. The resulting batches have been characterized using disc centrifuge photosedimentometry and small-angle X-ray scattering. The size distribution is shown to be a sensitive function of the molar ratio of the reactive PEG macromonomer to fluorinated monomer, and with some optimization latices of very low polydispersity can be obtained with this simple synthesis method. For short PEG grafts too high a molar ratio results in a build up of smaller size particles and a broadening of the size distribution, whereas for longer grafts the mean particle size increases with decreasing molar ratio.

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Core–shell fluorinated methacrylate nanoparticles with Rhodamine-B for confocal microscopy and fluorescence correlation spectroscopy applications

Cited by 14 publications

References 26 publications

Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability

Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability

pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells

Semi-batch synthesis of colloidal spheres with fluorinated cores and varying grafts of poly(ethylene glycol)

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