“…Mammary core clock genes’ expression was also shifted between day and night restricted feeding which was accompanied by changes in the timing of de novo fatty acid synthesis in the gland ( Salfer and Harvatine, 2020 ). In support of a role of mammary clock in regulating fatty acid synthesis and metabolism, our ChIP-seq analysis of transcriptional targets of BMAL1 in mouse mammary epithelial cells identified genes that regulate uptake, transport and synthesis of lipids ( Casey et al, 2021 ). Moreover, genes that regulate fatty acid synthesis were shown to exhibit circadian rhythms of expression in lactating mammary tissue ( Maningat et al, 2009 ), and our studies of mid-lactation cows found circadian disruption due to exposure to continuous light-dark phase shifts decreased expression of fatty acid synthase (FASN ) and acetyl CoA-carboxylase (ACACA) in the mammary gland ( Casey et al, 2014a ).…”