Cord–Blood–Derived Human Cultured Mast Cells Produce Interleukin 13 in the Presence of Stem Cell Factor

Abstract

Background: Mast cells have been regarded as a potential source of cytokines. Although the human mast cell line HMC–1 and human lung mast cells have been shown to produce interleukin (IL) 13, it still remains uncertain whether cord–blood–derived human cultured mast cells produce IL–13. Methods: Human cultured mast cells were raised from cord blood cells in the presence of stem cell factor (SCF) and IL–6. Levels of IL–13 mRNA were examined by a semiquantitative reverse transcriptase–polymerase chain reaction. IL–13 levels in the supernatants were measured with an enzyme–linked immunosorbent assay. Results: When the IgE–sensitized cultured mast cells were activated with anti–IgE, mRNA for IL–13 was amplified with a peak at 3 h after the stimulation. IL–13 was not detected in the supernatants of the activated mast cells in the absence of SCF, whereas the mast cells secreted significant amounts of IL–13 after the stimulation in the presence of SCF. Calcium ionophore A23187 also stimulated the mast cells to release IL–13 into the supernatant in the presence of SCF. Conclusions: These observations suggest that human mast cells can produce IL–13 under the condition with SCF. The cord–blood–derived human cultured mast cells will help in studying the functional properties of human mast cells in allergic diseases.

The Early Growth Response Factor-1 Is Involved in Stem Cell Factor (SCF)-induced Interleukin 13 Production by Mast Cells, but Is Dispensable for SCF-dependent Mast Cell Growth

“…It has been implicated in the induction of airway hyperresponsiveness and mucus hypersecretion (37,38). SCF may play an essential role in the regulation of IL-13 production because anti-IgE-induced IL-13 production by human mast cells is dependent on SCF (39). In addition, inhibition of SCF induced decreased airway hyper-responsiveness (40).…”

Nerve growth factor prevents apoptosis of cord blood‐derived human cultured mast cells synergistically with stem cell factor

“…Total cellular RNA was extracted by the method of acid guanidinium thiocyanate (GT)-phenol-chloroform extraction [27,28]. Oligonucleotide primers were synthesized by Kurabou Co. (Kurashiki, Japan) and their sequences are summarized as follows; p140 trk primers were 5 H -TGGAG AAGAA GGACG AAACA-3 H (sense, located at nucleotides 1277±1296) and 5 H -GCCTT GACAG CCACC AGCAT-3 H (antisense, at 1669±1688); p75 LNGFR primers, 5 H -GACAG TGGCA TCTCC GTGGA CA-3 H (sense, at 1023±1044) and 5 H -ACACC GGGGA TGTGG CAGTG GA-3 H (antisense, at 1374±1395).…”

Lipopolysaccharide-induced Stem Cell Factor Messenger RNA Expression and Production in Odontoblast-like Cells

“…SCF has been shown to induce hematopoietic cell activation and interleukin (IL)-13 and IL-6 release (21,22). In the present work, OD-like cells were found to produce SCF after contact with a bacterium-derived antigen.…”