Nerve growth factor prevents apoptosis of cord blood‐derived human cultured mast cells synergistically with stem cell factor

Kurosawa,; Miyachi,; Kanbe,; Saitoh,; Matsuda,

doi:10.1046/j.1365-2222.2000.00866.x

Cited by 55 publications

(33 citation statements)

References 36 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, we found that FB supernatants act synergistically with suboptimal concentrations of rSCF (0.5-5 ng/ml) but, in contrast to IL-3 and IL-4 (19, 21), they did not enhance MC survival in the presence of optimal (50 ng/ml) rSCF concentrations (not shown). NGF known to be produced by fibroblasts (27) has been shown to prevent apoptosis in human cord blood-derived mast cells in a SCF-dependent manner (28). However, in our experiments a neutralizing anti-NGF mAb did not alter MC survival in the presence of FB supernatants (Table III).…”

Section: Characterization Of Fb-derived MC Growth Factorscontrasting

confidence: 54%

Human Intestinal Fibroblasts Prevent Apoptosis in Human Intestinal Mast Cells by a Mechanism Independent of Stem Cell Factor, IL-3, IL-4, and Nerve Growth Factor

Sellge¹,

Lorentz²,

Gebhardt³

et al. 2004

The Journal of Immunology

View full text Add to dashboard Cite

In rodents, fibroblasts (FBs) mediate stem cell factor (SCF)-dependent growth of mast cells (MCs). In humans, SCF is mandatory for MC differentiation and survival. Other factors such as IL-3, IL-4, and nerve growth factor (NGF) act in synergism with SCF, thus enhancing proliferation and/or preventing apoptosis in MCs. In this study, we studied in vitro interactions between human MCs and human FBs, both isolated from the intestine and purified to homogeneity. In coculture with FBs, MCs survived for up to 3 wk, whereas purified MCs cultured alone died within a few days. TNF-α and IL-1β, which both did not affect MC survival directly, enhanced FB-dependent MC growth. We provide evidence that FB-derived MC growth factors are soluble, heat-sensitive molecules which down-regulate MC apoptosis without enhancing MC proliferation. However, only low amounts of SCF were measured in FB-conditioned medium (<0.2 ng/ml). Moreover, blocking of SCF/c-kit interaction by anti-SCF or anti-c-kit Abs and neutralization of IL-3, IL-4, and NGF did not affect MC survival in the coculture system. In conclusion, our data indicate that human FBs promote survival of human MCs by mechanisms independent of SCF, IL-3, IL-4, and NGF. Such interactions between MCs and FBs may explain why MCs accumulate at sites of inflammatory bowel disease and intestinal fibrosis.

show abstract

Section: Characterization Of Fb-derived MC Growth Factorscontrasting

confidence: 54%

Human Intestinal Fibroblasts Prevent Apoptosis in Human Intestinal Mast Cells by a Mechanism Independent of Stem Cell Factor, IL-3, IL-4, and Nerve Growth Factor

Sellge¹,

Lorentz²,

Gebhardt³

et al. 2004

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…9 NGF has been shown to induce upregulation of early growth response genes and other proliferation and survival signals in HMC-1 via TrkA 9,18-20 and rescue HMC-1 (V560G c-kit) cells from cell death mediated by imatinib mesylate. NGF, together with SCF, 23 could also promote growth, differentiation, and survival of cord blood-derived MCs 9,19,20,23 and murine MCs. 25,41 Thus, increased NGF serum levels, together with elevated expression of NGF and TrkA on SMCs in patients with mastocytosis and an increased migration of CD117 1 cells from the blood toward an NGF gradient via TrkA, might contribute to the increased numbers of MCs via increased migration and proliferation of MC progenitors.…”

Section: Discussionmentioning

confidence: 99%

“…48 However, the expression and, presumably, functions of NTs and their highaffinity receptors for NTs differ between MCs from humans and mice and also between different human tissues and human cell lines. 9,14,15,[18][19][20][21][22][23][24][25][26]48 In our study, we could demonstrate for the first time significantly higher circulating levels of the NTs NGF-b, NT-3, and NT-4 and increased migration of CD117 1 cells from the blood toward an NGF gradient via TrkA. NTs might contribute to the augmented MC tissue infiltration in mastocytosis via increased migration of MC progenitor cells toward NGF and/or stimulation of MC differentiation and proliferation by NGF and NT-3.…”

Section: Discussionmentioning

confidence: 99%

Increased circulating levels of neurotrophins and elevated expression of their high-affinity receptors on skin and gut mast cells in mastocytosis

Peng

Maintz

Allam

et al. 2013

Blood

View full text Add to dashboard Cite

show abstract

“…NGF may also act on mast cells, e.g. by preventing their apoptosis (Welker et al 1998, Kanbe et al 2000, Meineke et al 2000, Dutta et al 2011.…”

Section: Htpcs Secrete the Neurotropic Factors (Pro)ngf And Gdnfmentioning

confidence: 99%

Human testicular peritubular cells: more than meets the eye

2013

View full text Add to dashboard Cite

In healthy men, several layers of inconspicuously flat cells and extracellular matrix (ECM) proteins build the wall of the seminiferous tubules. The cells of this wall, peritubular cells, are not well characterized. They are smooth-muscle-like and contractile and transport immotile sperm, a function important for male fertility. However, their full functional importance, especially their potential contribution to the paracrine regulation of the male gonad, is unknown. In men with impaired spermatogenesis, the architecture of the tubular wall is frequently altered. Deposits of ECM and morphological changes of peritubular cells imply that functions of peritubular cells may be fundamentally altered. To be able to study human peritubular cells and their functions, a culture method was established. It is based on small biopsies of patients with obstructive azoospermia but normal spermatogenesis (human testicular peritubular cells, HTPCs) and non-obstructive azoospermia, impaired spermatogenesis, and testicular fibrosis (HTPCFs). Results obtained from cellular studies and parallel examinations of biopsies provide insights into the repertoire of the secretion products, contractile properties, and plasticity of human peritubular cells. They produce ECM components, including the proteoglycan decorin, which may influence paracrine signaling between testicular cells. They may contribute to the spermatogonial stem cell niche via secreted factors. They are regulated by mast cell and macrophage products, and in response produce factors that can fuel inflammatory changes. They possess a high degree of plasticity, which results in hypertrophy and loss of contractile abilities. The data collectively indicate important roles of inconspicuous testicular peritubular cells in human male fertility and infertility.Reproduction (2013) 145 R107-R116

show abstract

Nerve growth factor prevents apoptosis of cord blood‐derived human cultured mast cells synergistically with stem cell factor

Abstract: NGF may act as a key factor to promote the survival of human mast cells synergistically with SCF through the prevention of apoptosis.

Cited by 55 publications

References 36 publications

Human Intestinal Fibroblasts Prevent Apoptosis in Human Intestinal Mast Cells by a Mechanism Independent of Stem Cell Factor, IL-3, IL-4, and Nerve Growth Factor

Human Intestinal Fibroblasts Prevent Apoptosis in Human Intestinal Mast Cells by a Mechanism Independent of Stem Cell Factor, IL-3, IL-4, and Nerve Growth Factor

Increased circulating levels of neurotrophins and elevated expression of their high-affinity receptors on skin and gut mast cells in mastocytosis

Human testicular peritubular cells: more than meets the eye

Contact Info

Product

Resources

About