Cord blood attached-segments are not homogeneous in post-thaw CD34+ cell viability and clonogenicity

Galindo, Cristian Camilo; Lozano, Diana María Vanegas; Rodríguez, Bernardo Camacho; Perdomo-Arciniegas, Ana-María

doi:10.1016/j.cryobiol.2018.03.010

Cited by 1 publication

(2 citation statements)

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“…This process is denominated confirmatory testing and the protocol was previously standardized in the bank for clinical purposes [27]. Confirmation of the distal segment as a representative sample of the UCB unit was also performed to guarantee reliability of the results [28].…”

Section: Resultsmentioning

confidence: 99%

“…For confirmatory testing, attached distal segments were extracted in a cryocart (CHART MVE Medical, Ball Ground, GA, USA), keeping the temperature below –150 °C during the whole procedure. The segments were thawed in sterile conditions with a previously standardized protocol [28]. Briefly, 100 µL of UCB obtained from the segment were diluted 1/8 in a HES-based media and incubated for 30 min.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Vanegas¹,

Galindo²,

Páez-Gutiérrez³

et al. 2019

IJMS

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Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%