Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Vanegas, Diana; Galindo, Cristian Camilo; Páez-Gutiérrez, Iván Aurelio; González-Acero, Lorena-Xiomara; Valderrama, Pavel Medina; Lozano, Juan-Camilo; Camacho‐Rodríguez, Bernardo; Perdomo-Arciniegas, Ana-María

doi:10.3390/ijms20194875

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…However, CD34＋ cell viability was significantly higher in the STCB group. It has been reported that TNC, viable CD45＋ and CD34＋ cells have a statistically significant correlation with CD34 viability after thawing [22]. In addition, a recent study suggested that CB transplantation with low TNC but high CD34＋ cells was associated with enhanced engraftment [23].…”

Section: Discussionmentioning

confidence: 98%

Improving Storage Policy in Korean Public Cord Blood Banks: Comparison of Quality between Long-Term and Short-Term Storage of Cord Blood

Lee

Kim

et al. 2020

KJBT

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Background: The decreased use of cord blood units (CBU) due to improvements in haploidentical transplantation is a financial burden for public cord blood banks. Currently, there is no guidance regarding the length of cryopreservation of CBU in Korean public banks. The relative quality of long-term storage CB (LTCB) and short-term storage CB (STCB) needs to be evaluated to establish a storage policy. Methods: Thirty-four and thirty-one units of CB cryopreserved for less than one year and up to 14∼15.5 years, respectively, in the Busan Gyeongnam Public Cord Blood Bank were assessed. The total nucleated cells (TNCs), CD34+ cell counts, and colony-forming units-granulocyte monocyte (CFU-GM) were examined. The cell viabilities were evaluated by Eosin-Y exclusion staining and 7-aminoactinomycin D flow cytometry. The number of stored Korean public CB units from 2000 to 2016 was determined and categorized according to TNCs. Results: The post-thawing viability of the STCBs measured by flow cytometry was consistently higher than that of the LTCBs (TNCs, 62.5% vs 57.3%; MNCs, 93.1% vs 88.9%; CD34+ cells 95.7% vs 94.0%). The CD34+ cell viability was significantly higher in STCB (P=0.03). The CFU-GM after thawing was higher in STCBs (61.5±23.4 vs 49.9±22.8 [0.95 mm 2 ] P=0.05). Of the 48,161 CB units stored until 2016, Dec, 9,493 (19.7%), which were stored until 2006, had been stored for more than 10 years. Conclusion: LTCB with a low number of cells (＜0.7×10 9 cells) should be considered to exclude from storage for therapeutic purposes to improve the storage efficiency.

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Section: Discussionmentioning

confidence: 98%

Improving Storage Policy in Korean Public Cord Blood Banks: Comparison of Quality between Long-Term and Short-Term Storage of Cord Blood

Lee

Kim

et al. 2020

KJBT

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“…The TNC and CD34 + cells in the UCB unit were limited. However, UCB had a less mature phenotype of CD34 + progenitors compared to adult marrow and peripheral blood grafts, which might have a higher proliferation potential than adult CD34 + cells [ 31 ]. Taken together, the UCB unit, as one of the grafts for allo-HSCT, could reduce the GVHD effect, enhance the GVL effect and promote engraftment.…”

Section: Discussionmentioning

confidence: 99%

Unrelated umbilical cord blood can improve the prognosis of haploidentical hematopoietic stem cell transplantation

Yang

Zhang

et al. 2022

Stem Cell Res Ther

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Background Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) is widely used as a curative treatment strategy for most types of hematological diseases. However, strategies for enhancing the graft versus leukemia (GVL) effect without aggravating the graft versus host disease (GVHD) effect are still being pursued. Methods A retrospective cohort study was performed to compare the outcomes between combined unrelated umbilical cord blood (UCB-haplo HSCT) and haplo HSCT. Results The results showed that neither acute GVHD (aGVHD) nor chronic GVHD (cGVHD) was increased in the UCB-haplo HSCT group, and the engraftment and infection rates were similar between the two groups. However, overall survival and progression-free survival were significantly improved, while transplantation-related mortality and relapse were significantly decreased in the UCB-haplo HSCT group by both univariate and multivariate analyses. Conclusion Our results indicated that the addition of a UCB unit could improve the prognosis of haplo-HSCT and enhance the GVL effect without increasing the incidence of GVHD. Trial registration The cohort study was retrospectively registered at https://www.chictr.org.cn as ChiCTR2100046681.

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“…There is an important experience in Colombia with UCB transplantation for pediatric patients and a higher inventory of cord blood donors with HLA high-resolution typing available in the public UCB bank which started 5 years ago (11). Since there is no unrelated bone marrow donor registry in the country, UCB turns out an important source along with haploidentical donors for patients who require transplantation.…”

Section: Introductionmentioning

confidence: 99%

Clinical Outcomes of Unrelated Umbilical Cord Blood Graft vs. Haploidentical Donor Transplantation: Critical Issues for an Adequate Comparison

Vanegas¹,

Niño-Quiroga²,

Chaparro³

et al. 2021

Front. Med.

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Unrelated umbilical cord blood (UCB) and haploidentical grafts have been used for allogeneic hematopoietic stem and progenitor cell (HSPC) transplantation in patients without a related or non-related human leukocyte antigen (HLA)-matched donor. The less stringent HLA-matching requirement in both sources raises an important possibility for patients in need of urgent transplantation to treat any hematological disease. Selection of the best alternative donor is a difficult task that will depend on donor criteria, center experience, patient disease conditions, and risk, among others. Most comparisons available in scientific publications between both graft sources are obtained from retrospective analysis in wide time windows and a heterogeneous number of patients, types of disease, disease stages, previous treatments, graft source, conditioning regimen, graft vs. host disease (GVHD) approach, and evaluable endpoints. There is also an evident impact of the economic traits since low-income countries must consider less expensive treatments to satisfy the needs of the patients in the most effective possible path. Therefore, haploidentical transplantation could be an appealing option, even though it has not been completely established if any chronic treatment derived from the procedure could become a higher cost. In Colombia, there is a huge experience in UCB transplantation especially in units of pediatric transplantation where benign indications are more common than in adults. Due to the availability of a public UCB bank and HLA high-resolution typing in Colombia, there is a wider inventory of cord blood donors. Unfortunately, we do not have an unrelated bone marrow donor registry, so UCB is an important source along with haploidentical transplantation to consider in decision-making. This minireview focuses on comparing the main issues associated with the use of both HSCP sources and provides tools for physicians who face the difficult decision between these alternative donor sources.

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Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Cited by 6 publications

References 43 publications

Improving Storage Policy in Korean Public Cord Blood Banks: Comparison of Quality between Long-Term and Short-Term Storage of Cord Blood

Improving Storage Policy in Korean Public Cord Blood Banks: Comparison of Quality between Long-Term and Short-Term Storage of Cord Blood

Unrelated umbilical cord blood can improve the prognosis of haploidentical hematopoietic stem cell transplantation

Clinical Outcomes of Unrelated Umbilical Cord Blood Graft vs. Haploidentical Donor Transplantation: Critical Issues for an Adequate Comparison

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