Copy number variants in kiwifruit ETHYLENE RESPONSE FACTOR/APETALA2 (ERF/AP2)-like genes show divergence in fruit ripening associated cold and ethylene responses in C-REPEAT/DRE BINDING FACTOR-like genes

Gunaseelan, Kularajathevan; McAtee, Peter A.; Nardozza, Simona; Pidakala, P.; Wang, Ruiling; David, Karine; Burdon, J.; Schaffer, Robert J.

doi:10.1371/journal.pone.0216120

Cited by 21 publications

(17 citation statements)

References 50 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Actinidia chinensis, 226 ERF-like genes were identified and 26% ofthe ERF-like genes were up-regulated by ethylene. 43 AdERF10, AdERF14, AdERF15, and AdERF75 were confirmed as putative activators, and AdERF7, AdERF8, AdERF19, and AdERF21 were confirmed as putative repressors in kiwifruit ripening. 44 In our study, H 2 S inhibited the gene expression of ERF5 (Achn319471 and Achn063061), ERF016 (Achn023091) and ERF003 (Achn187281 and Achn134411), and increased the gene expression of ERF113 (Achn364871) and ERF4 (Achn362941) (Table S4).…”

Section: Discussionmentioning

confidence: 84%

“…Ethylene response factor (ERF) as the final response gene in the ethylene signal transduction pathway. In Actinidia chinensis, 226 ERF‐like genes were identified and 26% ofthe ERF‐like genes were up‐regulated by ethylene . AdERF10, AdERF14, AdERF15, and AdERF75 were confirmed as putative activators, and AdERF7, AdERF8, AdERF19, and AdERF21 were confirmed as putative repressors in kiwifruit ripening .…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Transcriptome analysis reveals delaying of the ripening and cell‐wall degradation of kiwifruit by hydrogen sulfide

et al. 2020

View full text Add to dashboard Cite

BACKGROUND Hydrogen sulfide (H2S) is a known signaling molecule in plants, which has the ability to delay fruit ripening. Our previous studies have shown that H2S treatment could delay the maturation of kiwifruits by inhibiting ethylene production, improving protective enzyme activities, and decreasing the accumulation of reactive oxygen species to protect the cell membrane during storage. The mechanism related to the way in which H2S affected kiwifruit maturation was still unclear. We performed transcriptome sequencing to explore the influences of H2S on the softening of kiwifruit. RESULTS The firmness and the soluble solids content (SSC) of the kiwifruit were significantly better maintained with H2S treatment compared to the control during the storage period (P < 0.05). Transmission electron microscopy (TEM) showed that degradation of the cell wall was inhibited after H2S treatment. Based on transcriptome data analysis and quantitative real‐time polymerase chain reaction (qRT‐PCR), expression levels of endo‐1,4‐β‐glucanase (β‐glu), β‐galactosidase (β‐gal) and pectinesterase (PME) decreased whereas pectinesterase inhibitor (PMEI) significantly increased in response to H2S. The members of the signal transduction pathway involved in ethylene were also identified. Hydrogen sulfide inhibited the expression of ethylene receptor 2 (ETR2), ERF003, ERF5, and ERF016, and increased the expression of ethylene‐responsive transcription factor 4 (ERF4) and ERF113. CONCLUSION Hydrogen sulfide could delay the ripening and senescence of kiwifruit by regulating the cell‐wall degrading enzyme genes and affecting ethylene signal transduction pathway genes. Our results revealed the effect of H2S treatment on the softening of kiwifruit at the transcription level, laying a foundation for further research. © 2020 Society of Chemical Industry

show abstract

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 96%

Transcriptome analysis reveals delaying of the ripening and cell‐wall degradation of kiwifruit by hydrogen sulfide

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To date, research on the regulation of postharvest ripening of kiwifruit has focused on changes in the content of endogenous hormones during this process, particularly the effects of ethylene and abscisic acid (ABA) [1][2][3]. The ripening and softening of kiwifruit were accelerated by ethylene and retarded by 1-MCP [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Application of Sucrose Promotes Postharvest Ripening of Kiwifruit

et al. 2020

View full text Add to dashboard Cite

Sucrose is an important component of fruit flavor, but whether sucrose signaling affects the postharvest ripening process of kiwifruit is unclear. The aim of this article was to study the effect of sucrose application on postharvest kiwifruit ripening to clarify the effect of sucrose in this process. Our present study found that exogenous sucrose can promote ethylene synthesis, which increases the ethylene content during fruit ripening, thereby accelerating the ripening and softening of kiwifruit after harvest. A significantly higher expression of AcACS1 and AcACO2 was found in sucrose-treated fruits compared to that in mannitol-treated fruits. Blocking the ethylene signal significantly inhibited the sucrose-modulated expression of most selected ripening-related genes. Sucrose transport is essential for sucrose accumulation in fruits; therefore, we isolated the gene family related to sucrose transport in kiwifruit and analyzed the gene expression of its members. The results show that AcSUT1 and AcTST1 expression increased with fruit ripening and AcSUT4 expression decreased with ripening, indicating that they may have different roles in the regulation of fruit ripening. Additionally, many cis-elements associated with phytohormones and sugar responses were found in the promoter of the three genes, which suggests that they were transcriptionally regulated by sugar signal and phytohormones. This study demonstrates the effect of sucrose on postharvest ripening of kiwifruit, providing a good foundation for further research.

show abstract

“…Within the fruit ripening ethylene associated turquoise module there were a considerable number (29) of NAC TFs identi ed, including the previously described NOR like genes (NAC1, NAC2, NAC3) [36], as well as seven of the eight EIN3-like genes. This module also included 45 ERF genes [26], as well as DOF4 [37,38]. A previous studies of fruit ripening analysis [37] described 10 TFs associated with ripening (Additional data 1), most of which were located in other coloured modules suggesting that the wider study presented here gives a better resolution of tissue speci c genes.…”

Section: Discussionmentioning

confidence: 87%

“…In kiwifruit, four classes of TFs have been previously reported, two (the R2R3 class MYBs [23] and WRKYs [24]) were based on a previous version of the kiwifruit genome, the third (the MICK type MADS-box genes) was published using EST sequence data [25] and the fourth (AP2/ERF class of gene) was based on the manually annotated genome [26]. The 96 published WRKY TFs were previously named based on sequential chromosomal locations.…”

Section: Mining Of Transcription Factor Gene Familiesmentioning

confidence: 99%

An Actinidia chinensis (kiwifruit) eFP browser and network analysis of transcription factors

Brian¹,

Warren²,

McAtee³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

BackgroundTranscriptomic studies combined with a well annotated genome have laid the foundations for new understanding of molecular processes. Tools which visualise gene expression patterns have further added to these resources. The manual annotation of the Actinidia chinensis (kiwifruit) genome has resulted in a high quality set of 33,044 genes. Here we investigate gene expression patterns in diverse tissues, visualised in an Electronic Fluorescent Pictograph (eFP) browser, to study the relationship of transcription factor (TF) expression using network analysis. ResultsSixty-one samples covering diverse tissues at different developmental time points were selected for RNAseq analysis and an eFP browser was generated to visualise this dataset. 2,839 TFs representing 57 different classes were identified and named. Network analysis of the TF expression patterns separated TFs into 14 different modules. Two modules consisting of 237 TFs were correlated with floral bud and flower development, a further two modules containing 160 TFs were associated with fruit development and maturation. A single module of 480 TFs was associated with ethylene-induced fruit ripening. Three “hub” genes correlated with flower and fruit development consisted of a HAF-like gene central to gynoecium development, an ERF and a DOF gene. Maturing and ripening hub genes included a KNOX gene that was associated with seed maturation, and a GRAS-like TF.ConclusionsThis study provides an insight into the complexity of the transcriptional control of flower and fruit development, as well as providing a new resource to the plant community. The eFP browser is provided in an accessible format that allows researchers to download and work internally.

show abstract

Copy number variants in kiwifruit ETHYLENE RESPONSE FACTOR/APETALA2 (ERF/AP2)-like genes show divergence in fruit ripening associated cold and ethylene responses in C-REPEAT/DRE BINDING FACTOR-like genes

Cited by 21 publications

References 50 publications

Transcriptome analysis reveals delaying of the ripening and cell‐wall degradation of kiwifruit by hydrogen sulfide

Transcriptome analysis reveals delaying of the ripening and cell‐wall degradation of kiwifruit by hydrogen sulfide

Exogenous Application of Sucrose Promotes Postharvest Ripening of Kiwifruit

An Actinidia chinensis (kiwifruit) eFP browser and network analysis of transcription factors

Contact Info

Product

Resources

About