Coproprevalencia de infestación canina por Echinococcus granulosus en un distrito endémico en hidatidosis en Perú

Montalvo, Raúl; Clemente, Johana; Castañeda, Lorena; Caro, Estephany; Ccente, Yoli; Nuñez, Mayori

doi:10.15381/rivep.v29i1.14189

Cited by 8 publications

(11 citation statements)

References 11 publications

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“…34 The ROC analysis uses both positive and negative samples and also showed optimal cutoff values depending on different scenarios of sensitivity and specificity that may be considered according to the prevalence of geographical regions worldwide. Considering the high prevalence of dog infection up to 50% in endemic areas of Peru, 35 our Copro-ELISA would have good PPV and NPV contributing to the detection of true positive and negative cases. Interlaboratory assays should be used to evaluate the reproducibility (stability) of our copro-ELISA, particularly in other countries with a prevalence of canine echinococcosis.…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Jara

Rodríguez

Altamirano

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme-linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus-soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-E. granulosus polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra-and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9-99.6) and 98.2% (CI: 89.5-100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7-100) and 98% (CI: 94.1-100), respectively. No cross-reactivity with Taenia hydatigena, Dipylidium caninum, or Toxocara canis was observed. Intra-and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with E. granulosus. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions.

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Section: Discussionmentioning

confidence: 99%

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Jara

Rodríguez

Altamirano

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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“…Una de ellas es la inoculación directa, muy discutida actualmente, ya que para que el huevo libere el embrión hexacanto necesita de contenido gastrointestinal (10) . En los dos casos reportados, Referencias Bibliográficas se describen lesiones quísticas múltiples en distintas zonas del cuerpo (antebrazos, muslo, glúteo y piernas), sin previo antecedente de lesión de piel en las zonas de aparición (11) .…”

Section: Discussionunclassified

Quiste hidatídico subcutáneo: reporte de dos casos en Huancayo, Perú

Pari

Almonacid

Gomez

et al. 2018

Rev Peru Med Exp Salud Publica

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La hidatidosis es una infección zoonótica que puede invadir múltiples órganos en el ser humano. Sin embargo, el tejido subcutáneo es el órgano menos afectado por esta enfermedad, cuando esto ocurre se aprecia escasa sintomatología, lo que ocasiona periodos prolongados de enfermedad asociado a diagnóstico tardío y a una pobre respuesta a la terapia antiparasitaria. La sierra central del Perú es la zona con mayor prevalencia, presumiblemente por las inadecuadas medidas de saneamiento ambiental, la falta de educación y las casi inexistentes medidas de control de la enfermedad. Con el objetivo de discutir los problemas relacionados a la localización subcutánea y su probable fisiopatología, reportamos dos casos de hidatidosis subcutánea que se presentaron con lesiones tumorales de evolución prolongada y que no afectaron al hígado ni a los pulmones y que finalmente fueron tratados mediante extirpación quirúrgica debido a la falta de respuesta al tratamiento con albendazol.

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“…In Peru, the practice of sacri cing adult sheep for domestic consumption and allowing dogs to feed on infected viscera generates the ideal conditions for the persistence of this disease [6][7]. In some rural areas the prevalence of infection can reach up to 50% [8].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antiparasitic therapy directly administered for the control of canine echinococcosis in an endemic area of hydatidosis.

Montalvo

Huiza

Clemente

et al. 2020

Preprint

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Background: Echinococcosis remains endemic despite attempts to reduce the prevalence of this disease. The dog faeces were analyzed by coproantigen using the immunoenzymatic technique for the diagnosis of echinococcosis before and after the direct and observed administration of praziquantel 5mg by kg orally in three doses every 30 days each cycle was 2 times at year for two consecutive years. The objective of the study was to evaluate the results of an intervention program based on antiparasitic therapy directly administered to the dogs for the control of Echinococcus in an endemic area of cystic echinococcosis (CE).Results: A total of 252 dogs entered the study, of them 119 (47%) dogs had positive results for Echinococcus at the beginning of the intervention. The adverse effects that occurred with the administration of prazicuantel were vomiting (13%), diarrhea (2.4%) and lethargy (2%); 229 dogs completed the 4 cycles of praziquantel treatment. The presence of a positive coproantigen was evident in 03 (1.3%) dogs at the end of the study.Conclusions: Antiparasitic chemotherapy administered directly to the dogs periodically managed to reduce the prevalence of canine echinococcosis.

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Coproprevalencia de infestación canina por Echinococcus granulosus en un distrito endémico en hidatidosis en Perú

Cited by 8 publications

References 11 publications

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Quiste hidatídico subcutáneo: reporte de dos casos en Huancayo, Perú

Evaluation of antiparasitic therapy directly administered for the control of canine echinococcosis in an endemic area of hydatidosis.

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