Coproantibodies to bacterial and viral enteropathogens in breast-fed infants

Kenny, Jean F.; Boesman, Mary; Micheals, Richard H.; Day, Richard L.

doi:10.1016/s0022-3476(65)81852-2

Cited by 21 publications

(28 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Specific antibody activity, therefore, appears to be maintained after processing, feeding, and passage of FM through the gastrointestinal tract. This has been observed for blood group agglutinins and antibodies to other microorganisms in human milk fed to infants (2,13,27).…”

Section: Resultsmentioning

confidence: 61%

“…Indeed, our assays did not detect any of the factors in the cow's milk-based formula. A further explanation for the greater excretion of immune factors by group FM may be due to the resistance of selected human milk proteins to proteolytic digestive enzymes (12,13). These possibilities were considered in more detail.…”

Section: Resultsmentioning

confidence: 99%

“…Fecal levels of these selected immune factors are greater m mfants fed human milk than in those fed a formula (1-3). In that regard, these selected factors also resist proteolytic digestion (12,13). These observations suggest that the factors remain intact throughout the infant's gastrointestinal tract.…”

mentioning

confidence: 91%

See 2 more Smart Citations

Enhanced Fecal Excretion of Selected Immune Factors in Very Low Birth Weight Infants Fed Fortified Human Milk

et al. 1986

View full text Add to dashboard Cite

SIgA, secretory IgA EID, electroimmunodiffusion ELISA, enzyme-linked immunosorbent assayThe decision to feed human milk to VLBW infants often is based on the potential enhancement of host defense. Two approaches have been used in the few studies d~signed to e.valuate the protection afforded by feeding human milk to these mfants: 1) assessment of morbidity and 2) the J?easuremen~of the concentrations of specific immune factors m serum, saliva, and feces of infants fed either human milk or cow's milk-based formulas (1-4). Most epidemiologic studies of morbidity in fullterm infants usually have concluded that breast-feeding is protective but interpretations of these reports often have been confo~nded by uncontrolled demographic variables (5). ",:ssessments of morbidity in VLBW infants have focused on sepSIS and necrotizing enterocolitis. No consistent benefits have been reported (6-8). Concentrations of immuno~obulinsin. the serum were similar in both groups or elevated m VLBW mfants fed cow's milk preparations (9-11).The effects of human milk feeding on fecal excretion of lactoferrin, lysozyme, and SIgA have been reporte~(~-3). Fecal levels of these selected immune factors are greater m mfants fed human milk than in those fed a formula (1-3). In that regard, these selected factors also resist proteolytic digestion (12, 13). These observations suggest that the factors remain intact throughout the infant's gastrointestinal tract. The few studies of immune factors in the feces of VLBW infants, however, have been of short duration and have not been designed to evaluate the effects of the quantity of immune factors fed, variations in nutrient intakes that would influence growth and maturation (1, 2), or intrinsic differences in the characte~stics ofth~population studied such as gastrointestinal absorptIve capaCIty. Furthermore the impact of augmented levels of lactoferrin, lysozyme, and SIgA in milk obtained from women delivering. prema~u~ely has not been considered in the design of most published climcal evaluations (14, 15). Ideally, a study of the effects of human milk on premature infants should be carried out wit~breast-fed infants. This study condition is precluded in VLBW mfants because of their difficulties in suckling and their nutritional needs that may not be met by the nutrient concentrations of human milk (16). " Our investigations of the effects of feedmg human mIlk to VLBW infants began with detailed assessments of the levels and stability of selected nutritional and immune factors during the collection, storage, heat processing, and fractionation of human milk (17-20). A protocol to fortify human milk was developed 711 Abbreviations ABSTRACT. The amounts of lactoferrin, lysozyme, total IgA, secretory IgA (SIgA), and specific SIgA antibodies to a pool of Escherichia coli 0 antigens were measured lD 96-h collections of feces obtained from 28 very low birth weight infants, 28-30 wk of gestation, studied at 2.5 and 6 wk of age. Eighteen of these infants were fed their mot~ers' milk fortified with fractions ...

show abstract

Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Fecal Excretion of Selected Immune Factors in Very Low Birth Weight Infants Fed Fortified Human Milk

et al. 1986

View full text Add to dashboard Cite

show abstract

“…Breast-fed infants obtain from colostrum, and later from milk, IgA-containing antibodies against all infections which the mother has met previously. Milk IgA could be detected in effective amounts in the stool of breast-fed infants, although it could not be found in feces of bottle-fed infants [16,17].…”

Section: Introductionmentioning

confidence: 99%

Serum Immunoglobulins and Coproantibody Formation in Infants after Artificial Intestinal Colonization with Escherichia coli 083 and Oral Lysozyme Administration

1973

View full text Add to dashboard Cite

“…Antiviral and antibacterial antibodies of the IgA class (6)(7)(8)(9) have been identified in these fluids, suggesting that exocrine IgA plays a role in the immune defenses of the gut. Because immunoglobulins in gastrointestinal fluids are exposed to potent proteolytic enzymes, their susceptibility to proteolytic degradation may influence their biological activities.…”

Section: Introduction Exocrine I1smentioning

confidence: 99%

Proteolytic degradation of exocrine and serum immunoglobulins

Brown¹,

Newcomb²,

Ishizaka³

1970

J. Clin. Invest.

162

View full text Add to dashboard Cite

A B S T R A C T The susceptibility of exocrine and serum immunoglobulins and antibodies to proteolytic degradation was assessed. Colostral and duodenal fluid exocrine 11S IgA, monomeric serum IgA, and IgG were digested with trypsin, chymotrypsin, or duodenal fluid. Exocrine IgA was more resistant to digestion than were the serum immunoglobulins. Under conditions of the experiments, most of colostral IgA retained its 11 S quaternary structure, including the secretory piece; the portion degraded was reduced almost entirely to peptides.The superior resistance of exocrine IgA was also demonstrated by digestion of serum IgG and nasal exocrine IgA diphtheria antitoxins with trypsin or duodenal fluid. Selective precipitation of trypsin-digested antitoxins with antibodies to heavy chains, light chains, or secretory piece revealed that the differences in susceptibility to digestion were due to differences in lability of the Fc portions of the IgA and IgG antibody molecules. The Fc portions of IgG antibody molecules were degraded or cleaved from the Fab units of the molecules, whereas the Fc-like portions of IgA antibody molecules remained associated with their Fab-like units and the secretory piece. On the other hand, trypsin treatment did not affect the antigen binding ability of the Fab parts of either the exocrine IgA or IgG antibodies.The Fc-like portions of exocrine IgA may be protected from tryptic degradation by the quaternary structure of the 11S molecules, which includes a dimer of 7S IgA subunits and the secretory piece.

show abstract

Coproantibodies to bacterial and viral enteropathogens in breast-fed infants

Cited by 21 publications

References 0 publications

Enhanced Fecal Excretion of Selected Immune Factors in Very Low Birth Weight Infants Fed Fortified Human Milk

Enhanced Fecal Excretion of Selected Immune Factors in Very Low Birth Weight Infants Fed Fortified Human Milk

Serum Immunoglobulins and Coproantibody Formation in Infants after Artificial Intestinal Colonization with Escherichia coli 083 and Oral Lysozyme Administration

Proteolytic degradation of exocrine and serum immunoglobulins

Contact Info

Product

Resources

About