Copper toxicity and carbohydrate production in the microalgae Cylindrotheca fusiformis and Gymnodinium sp.

Pistocchi, Rossella; Guerrini, Franca; Balboni, V.; Boni, Leonardo

doi:10.1017/s0967026297001170

Cited by 23 publications

(30 citation statements)

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“…3a) produced increased with increasing Pi depletion and the same was observed for polysaccharides measured in the extracellular fraction (Fig. 3b); this fraction can be regarded as a polysaccharide fraction in that up to 70-80% of it consists of polymeric residues (Pistocchi et al 1997). …”

Section: Polysaccharide Productionsupporting

confidence: 71%

“…In particular, total carbohydrates were measured by adding 50 p1 80 % phenol and 5 m1 95 % sulphuric acid to 1 m1 culture supplemented with 1 m1 filtered seawater (salinity 25%0). The extracellular carbohydrate fraction was analyzed as previously described (Pistocchi et al 1997); briefly, 5 m1 cultures were acidified with 20 p1 1 N HCl and centrifuged at 10200 X g for 10 min in a Beckman J2-HS centrifuge (rotor JS-13.1) to pellet the cells, then 2 m1 of the supernatant was analyzed by the phenol-sulfuric acid method.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial-algal interactions in polysaccharide production

Guerrini¹,

Mazzotti²,

Boni³

et al. 1998

Aquat. Microb. Ecol.

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ABSTRACT. The effects of phosphate limitation and of the presence of marine bacteria during phosphate limitation on growth and polysaccharide production in the diatom Cylindrotheca fusiformis grown in batch cultures are described in this paper. Growth of C. fusiformis was inhibited under low Pi (inorganic phosphate) conditions, corresponding to an increasing N/P ratio, and higher amounts of polysaccharides were extruded in the medium, in particular during the stationary phase of growth. The presence of bacteria reduced phytoplankton cell density only when the phosphate added corresponded to 1/6 of the initial amount. Even when diatom cell growth was not affected, the presence of bacteria stimulated a higher polysaccharide production. These results are interpreted in the light of the fact that nutrient-stressed phytoplankton cells produced and released a higher amount of polysaccharides and, as bacteria exhibited a better utilization of phosphate than algal cells, their presence accentuated the Pi depletion, resulting in a higher polysaccharide production.

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Section: Polysaccharide Productionsupporting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Bacterial-algal interactions in polysaccharide production

Guerrini¹,

Mazzotti²,

Boni³

et al. 1998

Aquat. Microb. Ecol.

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“…The total soluble sugars were quantified according to the phenol-sulfuric acid method of Pistocchi et al (1997) using glucose as a standard. Phenol 1 ml (5%) and 5 ml sulfuric acid (98%) were added to 2 ml of the supernatant from the general extraction and preparation.…”

Section: Determination Of Total Soluble Sugarsmentioning

confidence: 99%

Effects of the exogenous application of auxin and cytokinin on carbohydrate accumulation in grains of rice under salt stress

et al. 2011

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Phytohormones, such as auxin and cytokinin, are known to be involved in the regulation of plant responses to salinity stress and counteract the adverse effect of stress conditions. This work investigated the effects of the exogenous spraying of indole-3-acetic acid (IAA) and kinetin (KIN) during the reproductive phase on grain yield by examining the 1000-grain weight and filledgrain percentage as well as the changes in starch, total soluble sugars, sucrose, glucose and fructose concentrations in the grains of two rice cultivars under salt stress. The results indicated that the applied IAA and KIN led to an increased grain yield, 1000-grain weight and filled-grain percentage for both rice cultivars under salt stress. The storage starch content in the grain of the salt-sensitive cultivar was more than that in the salt-tolerant cultivar under IAA application compared with KIN, whereas a decrease in the total soluble sugar content was observed with both IAA and KIN treatments, in comparison to the non-hormone treatment. Interestingly, this study showed that IAA led to a much higher increase in the sucrose content in grain, as compared to the KIN. Furthermore, this experiment suggests that glucose and fructose may play important roles during salt stress because there were clearly higher concentrations of these sugars in the grain of the stressed cultivars under IAA and KIN application: it appears that their accumulation was the earliest response detected during the grain-filling period in rice. Finally, this work indicated that an increase in the rice grain yield, 1000-grain weight and filled-grain percentage are associated with an increase in the contents of starch, sucrose, glucose and fructose in grain caused by the application of IAA and KIN.

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“…The supernatants obtained for the second method were concentrated in a rotary evaporator (40 °C, 120 r/min), then dialyzed in a dialysis tube (1 000 Da). The solutions were freeze-dried and prepared as EPS (Pistocchi et al 1997).…”

Section: Growth Curve and Carbohydrate Measurementsmentioning

confidence: 99%

“…Carbohydrates were quantified according to the phenolsulphuric acid method of Pistocchi et al (1997), using glucose as a standard. Cellular total carbohydrates and reducing sugars were prepared according to the method of Li et al (2001), whereas water-soluble sugars and sucrose were quantified according to the method of Tang (1999).…”

Section: Growth Curve and Carbohydrate Measurementsmentioning

confidence: 99%

Effects of Salt Stress on Carbohydrate Metabolism in Desert Soil Alga Microcoleus vaginatus Gom.

Chen

Song

et al. 2006

JIPB

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The effects of salt stress on carbohydrate metabolism in Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crusts, were investigated in the present study. Extracellular total carbohydrates and exopolysaccharides (EPS) in the culture medium produced by M. vaginatus increased significantly during the growth phase and reached a maximum during the stationary phase. The production of extracellular carbohydrates also significantly increased under higher salt concentrations, which was attributed to an increase in low molecular weight carbohydrates. In the presence of NaCl, the production of cellular total carbohydrates decreased and photosynthetic activity was impaired, whereas cellular reducing sugars, water-soluble sugars and sucrose content and sucrose phosphate synthase activity increased, reaching a maximum in the presence of 200 mmol/L NaCl. These parameters were restored to original levels when the algae were transferred to a non-saline medium. Sodium and K + concentrations of stressed cells decreased significantly and H + -ATPase activity increased after the addition of exogenous sucrose or EPS. The results suggest that EPS and sucrose are synthesized to maintain the cellular osmotic equilibrium between the intra-and extracellular environment, thus protecting algal cells from osmotic damage, which was attributed to the selective exclusion of cellular Na + and K + by H + -ATPase.

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Copper toxicity and carbohydrate production in the microalgae Cylindrotheca fusiformis and Gymnodinium sp.

Cited by 23 publications

References 0 publications

Bacterial-algal interactions in polysaccharide production

Bacterial-algal interactions in polysaccharide production

Effects of the exogenous application of auxin and cytokinin on carbohydrate accumulation in grains of rice under salt stress

Effects of Salt Stress on Carbohydrate Metabolism in Desert Soil Alga Microcoleus vaginatus Gom.

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