Copper ions regulate cytotoxicity of disulfiram to myeloid leukemia cells

Navrátilová, Jarmila; Jungová, Pavla; Vaňhara, Petr; Preisler, Jan; Kanický, Viktor; Šmarda, Jan

doi:10.3892/ijmm_00000277

Cited by 7 publications

(1 citation statement)

References 23 publications

(35 reference statements)

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“…For this reason, it is necessary to select a technique that bridges the obstacles mentioned above. Such a technique is Substrate-Assisted Laser Desorption Inductively Coupled Plasma Mass Spectrometry (SALD ICP-MS), which is capable of analysing liquid samples of submicrolitre volumes in the form of dried droplets (stains) on a polyethylene terephthalate (PET) substrate [1][2][3]. The greatest difficulty of this technique is the low repeatability of the assay.…”

Section: Introductionmentioning

confidence: 99%

Cold Plasma: The Way to Improve the Repeatability of Sald ICP-MS Analysis

Stiborek¹,

Preisler²,

Shekargoftar³

et al. 2018

Hungarian Journal of Industry and Chemistry

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This work deals with the preparation of model biological microsamples for Substrate-Assisted Laser Desorption Inductively Coupled Plasma Mass Spectrometry (SALD ICP-MS). This technique provides the direct and fast analysis of liquid samples deposited onto polyethylene terephthalate (PET) plates (substrates) in the form of dried droplets with minimal sample preparation and submicrolitre sample volume requirements. Furthermore, SALD ICP-MS allows for the direct analysis of samples in organic solvents, which cannot be directly nebulised to ICP-MS. These benefits are, however, balanced out by the low degree of repeatability of the assay, which is typically about 30%. One of the approaches to increase the repeatability is a modification of the substrate surface by Diffuse Coplanar Surface Barrier Discharge (DCSBD) with the addition of an internal standard to the sample. Using this approach, a relative standard deviation (RSD) of less than 10% for model biological samples can be achieved. The only limitation of this technique is the occurrence of the so-called “aging effect”, i.e. the gradual return of the modified physicochemical surface properties to their original state

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Section: Introductionmentioning

confidence: 99%

Cold Plasma: The Way to Improve the Repeatability of Sald ICP-MS Analysis

Stiborek¹,

Preisler²,

Shekargoftar³

et al. 2018

Hungarian Journal of Industry and Chemistry

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Mechanisms associated with cuproptosis and implications for ovarian cancer

Chen,

Liu

2024

Journal of Inorganic Biochemistry

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A Copper-Mediated Disulfiram-Loaded pH-Triggered PEG-Shedding TAT Peptide-Modified Lipid Nanocapsules for Use in Tumor Therapy

Zhang

Tian

et al. 2015

ACS Appl. Mater. Interfaces

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Disulfiram, which exhibits marked tumor inhibition mediated by copper, was encapsulated in lipid nanocapsules modified with TAT peptide (TATp) and pH-triggered sheddable PEG to target cancer cells on the basis of tumor environmental specificity. PEG-shedding lipid nanocapsules (S-LNCs) were fabricated from LNCs by decorating short PEG chains with TATp (HS-PEG(1k)-TATp) to form TATp-LNCs and then covered by pH-sensitive graft copolymers of long PEG chains (PGA-g-PEG(2k)). The DSF-S-LNCs had sizes in the range of 60-90 nm and were stable in the presence of 50% plasma. DSF-S-LNCs exhibited higher intracellular uptake and antitumor activity at pH 6.5 than at pH 7.4. The preincubation of Cu showed that the DSF cytotoxicity was based on the accumulation of Cu in Hep G2 cells. Pharmacokinetic studies showed the markedly improved pharmacokinetic profiles of DSF-S-LNCs (AUC= 3921.391 μg/L·h, t(1/2z) = 1.294 h) compared with free DSF (AUC = 907.724 μg/L·h, t(1/2z) = 0.252 h). The in vivo distribution of S-LNCs was investigated using Cy5.5 as a fluorescent probe. In tumor-bearing mice, the delivery efficiency of S-LNCs was found to be 496.5% higher than that of free Cy5.5 and 74.5% higher than that of LNCs in tumors. In conclusion, DSF-S-LNCs increased both the stability and tumor internalization and further increased the cytotoxicity because of the higher copper content.

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Copper ions regulate cytotoxicity of disulfiram to myeloid leukemia cells

Cited by 7 publications

References 23 publications

Cold Plasma: The Way to Improve the Repeatability of Sald ICP-MS Analysis

Cold Plasma: The Way to Improve the Repeatability of Sald ICP-MS Analysis

Mechanisms associated with cuproptosis and implications for ovarian cancer

A Copper-Mediated Disulfiram-Loaded pH-Triggered PEG-Shedding TAT Peptide-Modified Lipid Nanocapsules for Use in Tumor Therapy

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