Copper-induced oxidative stress in Alternanthera philoxeroides callus

Xu, Xiaoying; Shi, Guoxin; Wang, Juan; Zhang, Lele; Kang, Yi-ning

doi:10.1007/s11240-010-9914-2

Cited by 23 publications

(13 citation statements)

References 41 publications

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“…However, unlike the intact plants, the mechanisms of heavy metal tolerance in callus cells are not fully elucidated, especially in relation to plant mitochondrial properties. Nevertheless, it is known that metal stress triggers the production of antioxidant, which acts a defense mechanism in callus cells (Gallego et al, 2002;Xu et al, 2011;Lukatkin et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

Mamat

ChunShiong²,

Samad³

et al. 2015

IJAB

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Plants need an appropriate amount of copper to sustain their normal growth. However, excess of copper may interrupt plant development and cause stress. In this study, the responses of Orthosiphon stamineus callus culture under various copper concentrations were investigated. Callus cells at 24-days old were treated with 100, 150, 200 and 250 µM of copper solution and harvested after one week. Mitochondria from the calluses were isolated and the total mitochondrial proteins were determined. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to compare the mitochondrial protein profiles in normal medium and copper-containing medium. The activities of cytochrome c oxidase, as well as the total phenolic and flavonoid contents were determined. The results of atomic absorption spectroscopy (AAS) analysis have shown significant amount of copper accumulated in O. stamineus callus cells, depending on the amount of copper supplied. With the BCF values of more than 10, this species can be categorized as copper hyperaccumulator. When the amount of copper supplied increased, the cells exhibit higher cytochrome c oxidase activities and produce more phenolic compounds, in spite of decreasing amount of total mitochondrial proteins and a lesser number of visible bands visualized from SDS-PAGE analysis. Overall, our findings highlight the dynamic responses of O. stamineus cells when exposed to various levels of copper, notably by alteration of biochemical and mitochondrial properties as their protection mechanism under stress.

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Section: Discussionmentioning

confidence: 99%

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

Mamat

ChunShiong²,

Samad³

et al. 2015

IJAB

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“…Heavy metals present at elevated levels may cause an increased production of reactive oxygen species (ROS) in plant cells on one hand [42][43][44][45][46][47][48][49][50][51] and may be involved in the enzymatic detoxification of ROS on the other hand [2,3,42,[52][53][54]. Stresses (e.g., drought, heat and high light intensity) may also cause an accumulation of ROS and of ROS-damaged cell constituents [54,55].…”

Section: Heavy Metals: Micronutrients or Pollutants?mentioning

confidence: 99%

Heavy Metals in Crop Plants: Transport and Redistribution Processes on the Whole Plant Level

2015

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Abstract:Copper, zinc, manganese, iron, nickel and molybdenum are essential micronutrients for plants. However, when present in excess they may damage the plant or decrease the quality of harvested plant products. Some other heavy metals such as cadmium, lead or mercury are not needed by plants and represent pollutants. The uptake into the roots, the loading into the xylem, the acropetal transport to the shoot with the transpiration stream and the further redistribution in the phloem are crucial for the distribution in aerial plant parts. This review is focused on long-distance transport of heavy metals via xylem and phloem and on interactions between the two transport systems. Phloem transport is the basis for the redistribution within the shoot and for the accumulation in fruits and seeds. Solutes may be transferred from the xylem to the phloem (e.g., in the small bundles in stems of cereals, in minor leaf veins). Nickel is highly phloem-mobile and directed to expanding plant parts. Zinc and to a lesser degree also cadmium are also mobile in the phloem and accumulate in meristems (root tips, shoot apex, axillary buds). Iron and manganese are characterized by poor phloem mobility and are retained in older leaves.

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“…The Mn-SOD is resistant to both inhibitors; Cu/Zn-SOD is sensitive to both inhibitors whereas; Fe-SOD is resistant to KCN and sensitive to H2O2 (Ahmad et al, 2008;Gill and Tuteja, 2010;Karuppanapandian et al, 2011). There have been many reports of the increased activities of SOD under abiotic stresses induced with tissue culture techniques in a wide range of plant species, including heavy metals, such as Al, Cd, Cr, and Cu, hyperhydricity, salinity, gamma radiation, and drought (Gallego et al, 2002;Saher et al, 2004;Dewir et al, 2006;Israr et al, 2006;Erturk et al, 2007;Dasgupta et al, 2008;Sivritepe et al, 2008;Gupta and Prasad, 2010;Shehab et al, 2010;Yang et al, 2010;El-Beltagi et al, 2011;Helaly and El-Hosieny , 2011;Lokhande et al, 2011;Sen and Alikamanoglu, 2011;Xu et al, 2011;Patada et al, 2012) on the other hand, Fe-deficiency stress reduced activity of SOD (Lombardi et al, 2003). Advanced-antioxidant defense systems play an important role in plants not only to tolerate environmental stress but also to improve plants against these stresses.…”

Section: Antioxidant Defence Systemmentioning

confidence: 99%

Oxidative Stress Studies in Plant Tissue Culture

Şen¹

2012

Antioxidant Enzyme

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Copper-induced oxidative stress in Alternanthera philoxeroides callus

Abstract: The callus of Alternanthera philoxeroides, an emerging plant, was subjected to different concentrations of Cu 2? (0, 0.05, 0.

Cited by 23 publications

References 41 publications

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

Heavy Metals in Crop Plants: Transport and Redistribution Processes on the Whole Plant Level

Oxidative Stress Studies in Plant Tissue Culture

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