Copper-enhanced gold nanoparticle tags for electrochemical stripping detection of human IgG

Mao, Xinhua; Jiang, Jian‐Hui; Luo, Yan; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.talanta.2007.04.004

Cited by 51 publications

(23 citation statements)

References 19 publications

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“…In order to improve the conductivity of the electrode, we brought in gold nanoparticles together with the probable improved retainment of the bioactivity of the immobilized antibody. Application of gold nanoparticles for modifying the electrode [25][26][27][28] could significantly decrease electron-tunneling distance, facilitating the electron transfer. Chitosan (CHIT) [29][30][31][32] is a kind of polysaccharide which is protonated and positively charged in solution.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Kang

Feng

Chen

et al. 2008

Bioelectrochemistry

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Section: Introductionmentioning

confidence: 99%

Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Kang

Feng

Chen

et al. 2008

Bioelectrochemistry

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“…The following preparation of AuNPs-anti-IgG conjugate was referred to literature with some alterations [25]. One mL of anti-IgG (1 mg/mL) was added into 10 mL AuNPs suspension with pH at 9.0 (0.1 M Na 2 CO 3 and 0.1 M HCl were used to adjust pH), and the mixture was incubated at room temperature for 1 h. Then 1 mL 10 % BSA solution was injected under stirring to block the unspecific binding of proteins to antibodies on AuNPs, followed by incubation at room temperature for another 0.5 h. The conjugate was centrifuged (16, 000 rpm) at 4°C for 15 min, and the soft sediment was washed and resuspended in PBSB solution.…”

Section: Preparation Of Aunps and Aunps-anti-igg Conjugatementioning

confidence: 99%

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

et al. 2015

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A naked-eye sensitive ELISA-like assay was developed based on gold-enhanced peroxidase-like activity of gold nanoparticles (AuNPs). Using human IgG (H-IgG) as an analytical model, goat anti-human IgG antibody (anti-IgG) adsorbed on microtiter plate and AuNPs-labeled anti-IgG acted as capture antibody and detection antibody, respectively. Because the surfaces of AuNPs were blocked by protein molecules, the peroxidase-like activity of AuNPs was almost inhibited, evaluated by the catalytic oxidation of peroxidase enzyme substrate 3,3′,5,5′-tetramethylbenzidine (TMB), which could produce a bright blue color in the presence of H 2 O 2 . Fortunately, the catalytic ability of AuNPs was dramatically increased by the deposition of gold due to the formation of a new gold shell on immunogold. Under optimal reaction conditions, the colorimetric immunoassay presented a good linear relationship in the range of 0.7-100 ng/mL and the limit of detection (LOD) of 0.3 ng/mL calculated by 3σ/S for UVvis detection, and obtained LOD of 5 ng/mL for naked-eye detection. The obtained results were competitive with conventional sandwich ELISA with the LOD of 1.6 ng/mL. Furthermore, this developed colorimetric immunoassay was successfully applied to diluted human serum and fetal bovine serum samples, and predicted a broad prospect for the use of peroxidase-like activity involving nanomaterials in bioassay and diagnostics.

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“…[16] with minor modifications. Briefly, 1 mL of 1% HAuCl 4 solution and 100 mL of doubly distilled water were added into a 250 mL round-bottom flask.…”

Section: Preparation Of Gold Nanoparticles and Antibody-colloidal Golmentioning

confidence: 99%

Highly sensitive electrochemical stripping detection of hepatitis B surface antigen based on copper-enhanced gold nanoparticle tags and magnetic nanoparticles

Shen

Zhang

2010

Analytica Chimica Acta

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Copper-enhanced gold nanoparticle tags for electrochemical stripping detection of human IgG

Cited by 51 publications

References 19 publications

Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

Highly sensitive electrochemical stripping detection of hepatitis B surface antigen based on copper-enhanced gold nanoparticle tags and magnetic nanoparticles

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