Highly sensitive electrochemical stripping detection of hepatitis B surface antigen based on copper-enhanced gold nanoparticle tags and magnetic nanoparticles

“…Magnetic bead is a recently developed revolutionary material that has been widely used in biological and pharmaceutical research, including targeted drug delivery (Amirfazli, 2007), fluoxetine isolation (Bagheri et al, 2011), ligand screening , immunoassay (Shen and Zhang, 2010), pathogen detection (Kim and Son, 2010) and drug-protein binding studies (Marszall, 2011;). Jonker et al (2009 developed an online screening system using commercially available Co(II)-paramagnetic affinity beads in combination with the His-tagged human estrogen-receptor ligand-binding domain, and characterized the system through a number of estrogenic ligands and nonbinding pharmaceutical compounds.…”

Section: Introductionmentioning

confidence: 99%

Rapid screening and identification of α‐glucosidase inhibitors from mulberry leaves using enzyme‐immobilized magnetic beads coupled with HPLC/MS and NMR

Tao

Zhang

Cheng

et al. 2012

Biomedical Chromatography

337

123

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α-Glucosidase plays important roles in the digestion and absorption of carbohydrates in the small intestine. The inhibition of α-glucosidase is regarded as a potential way to treat diabetes. We established an approach to screening α-glucosidase inhibitors from medicinal plants using enzyme-coated magnetic bead. Using 1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide as reaction reagents, α-glucosidase was immobilized on the magnetic beads by covalent linkage. The conjugation of α-glucosidase to the magnetic beads was characterized using scanning electron microscope and X-ray diffractometer. The proposed approach was applied in fishing potential α-glucosidase inhibitors from extract of Morus alba, a Chinese medicinal plant. The structures of potential active compounds were identified via liquid chromatography-mass spectrometry and nuclear magnetic resonance. The results demonstrated that two flavonoids (isoquercitrin and astragalin) could bind to α-glucosidase, which was confirmed via conventional α-glucosidase inhibitory assay. Our findings suggested that enzyme-coated magnetic beads may be suitable for discovering active compounds from medicinal plants.

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“…Alizadeh et al designed an electrochemical HBsAg immunosensor by assembling anti-HBsAg coated MNPs on an electrode as a supporting matrix, and peroxidase-labelled AuNPs were used to generate a voltametrically detectable signal [ 77 ], with a LOD of 0.19 pg/mL HBsAg. Shen et al combined AuNPs with MNPs to detect HBsAg by anodic stripping voltammetry, with a LOD of 87 pg/mL [ 75 ].…”

Section: Combinations Of Different Nanoparticlesmentioning

confidence: 99%

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

Shevtsov

Zhao

Protzer

et al. 2017

Viruses

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Chronic infection with the hepatitis B virus (HBV) can lead to liver failure and can cause liver cirrhosis and hepatocellular carcinoma (HCC). Reliable means for detecting and monitoring HBV infection are essential to identify patients in need of therapy and to prevent HBV transmission. Nanomaterials with defined electrical, optical, and mechanical properties have been developed to detect and quantify viral antigens. In this review, we discuss the challenges in applying nanoparticles to HBV antigen detection and in realizing the bio-analytical potential of such nanoparticles. We discuss recent developments in generating detection platforms based on gold and iron oxide nanoparticles. Such platforms increase biological material detection efficiency by the targeted capture and concentration of HBV antigens, but the unique properties of nanoparticles can also be exploited for direct, sensitive, and specific antigen detection. We discuss several studies that show that nanomaterial-based platforms enable ultrasensitive HBV antigen detection.

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Highly sensitive electrochemical stripping detection of hepatitis B surface antigen based on copper-enhanced gold nanoparticle tags and magnetic nanoparticles

Cited by 35 publications

References 18 publications

Gold Nanoparticles in Chemical and Biological Sensing

Gold Nanoparticles in Chemical and Biological Sensing

Rapid screening and identification of α‐glucosidase inhibitors from mulberry leaves using enzyme‐immobilized magnetic beads coupled with HPLC/MS and NMR

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

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