Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity

Wu, Chengtie; Zhou, Yinghong; Xu, Mengchi; Han, Pingping; Chen, Lei; Chang, Jiang; Xiao, Yin

doi:10.1016/j.biomaterials.2012.09.066

Cited by 712 publications

(516 citation statements)

References 51 publications

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“…Moreover, copper (Cu 2þ ) ions are considered metallic angiogenic factors, because of their important role in blood vessel growth [21,[52][53][54] and the stimulation of the proliferation of endothelial cells [21,[55][56][57][58]. In addition, Cu 2þ ions also enhance the differentiation of stem cells towards the osteogenic linage [54,59]. Furthermore, Cu 2þ is a natural antimicrobial material [45,47] and also reported was a synergistic antimicrobial effect with Ca 2þ [60].…”

Section: Introductionmentioning

confidence: 99%

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Cattalini

Hoppe

Pishbin

et al. 2015

J. R. Soc. Interface.

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This work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu 2þ or Ca 2þ (AlgNbgCu, AlgNbgCa, respectively). Twodimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu 2þ and Ca 2þ are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu 2þ or Ca 2þ were developed with potential applications for preparation of multifunctional scaffolds for BTE.

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Section: Introductionmentioning

confidence: 99%

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Cattalini

Hoppe

Pishbin

et al. 2015

J. R. Soc. Interface.

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“…16 It also plays important roles in multiple systems, including the nervous system, cardiovascular system, and immune system. 17 Also, recent intensive research has revealed that Cu provides good antibacterial, [18][19][20] osteogenic, 20 and antineoplastic properties, 21 which is potentially advantageous for the treatment of orthopedic diseases. Unfortunately, Cu is not directly used as an implant material in clinical applications, except for in female intrauterine devices, 22 due to its inflammatory property and cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Wang¹,

Li²,

Ren³

et al. 2017

IJN

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Treatment for fractures requires internal fixation devices, which are mainly produced from stainless steel or titanium alloy without biological functions. Therefore, we developed a novel nano-copper-bearing stainless steel with nano-sized copper-precipitation (317L-Cu SS). Based on previous studies, this work explores the effect of 317L-Cu SS on fracture healing; that is, proliferation, osteogenic differentiation, osteogenesis-related gene expression, and lysyl oxidase activity of human bone mesenchymal stem cells were detected in vitro. Sprague–Dawley rats were used to build an animal fracture model, and fracture healing and callus evolution were investigated by radiology (X-ray and micro-CT), histology (H&E, Masson, and safranin O/fast green staining), and histomorphometry. Further, the Cu 2+ content and Runx2 level in the callus were determined, and local mechanical test of the fracture was performed to assess the healing quality. Our results revealed that 317L-Cu SS did not affect the proliferation of human bone mesenchymal stem cells, but promoted osteogenic differentiation and the expression of osteogenesis-related genes. In addition, 317L-Cu SS upregulated the lysyl oxidase activity. The X-ray and micro-CT results showed that the callus evolution efficiency and fracture healing speed were superior for 317L-Cu SS. Histological staining displayed large amounts of fibrous tissues at 3 weeks, and cartilage and new bone at 6 weeks. Further, histomorphometric analysis indicated that the callus possessed higher osteogenic efficiency at 6 weeks, and a high Cu 2+ content and increased Runx2 expression were observed in the callus for 317L-Cu SS. Besides, the mechanical strength of the fracture site was much better than that of the control group. Overall, we conclude that 317L-Cu SS possesses the ability to increase Cu 2+ content and promote osteogenesis in the callus, which could accelerate the callus evolution process and bone formation to provide faster and better fracture healing.

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“…Stimulating infiltration of blood vessels into a bone substitute scaffold, therefore, could increase the viability of bone forming cells within the scaffold and so hasten the healing process. Recently, the preparation of CuSO4-loaded calcium phosphate scaffolds and of Cu-doped solgel bioactive glasses for this purpose has been reported [2,3]. Here we report the preparation of hydroxyapatite structurally doped with Cu ions, with the aim of developing a bio-instructive scaffold.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Copper-Doped Hydroxyapatite with Varying x in the Composition Ca10(PO4)6CuxOyHz

Imrie¹,

Skakle²,

Gibson³

2013

Bioceram Dev Appl

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Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity

Cited by 712 publications

References 51 publications

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

Preparation of Copper-Doped Hydroxyapatite with Varying x in the Composition Ca10(PO4)6CuxOyHz

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