Copper-containing mesoporous bioactive glass promotes angiogenesis in an in vivo zebrafish model

Abstract

Mesoporous bioactive glasses (MBGs) with high specific surface area, well-ordered pores, large pore volumes and controllable amount of ions are interesting to develop controlled drug delivery systems for bone tissue regeneration. Copper (Cu) incorporation to the basic SiO-CaO-PO composition has attracted high interest due to its multifunctional biological properties. Promotion of angiogenesis is one of these properties, which can be integrated to the biomaterial with lower cost and higher stability when compared with growth factors. This work reports the synthesis and characterization of Cu-containing MBG evaluating its angiogenic properties in the subintestinal vessel zebrafish assay. This transgenic in vivo assay is merging as an alternative model providing short-time consuming protocols and facilities during pro-angiogenic drug screenings. The report shows that the ionic products of this MBG material delivered to the zebrafish incubation media significantly enhance angiogenesis in comparison with control groups. Besides, results indicate Cu ions may exhibit a synergic effect with Si, Ca, and P ions in angiogenesis stimulation both in vitro and in vivo. To our knowledge, this is the first time that zebrafish in vivo assays are used to evaluate angiogenic activity of ionic dissolution products from MBG materials.

Strontium- and Cobalt-Doped Multicomponent Mesoporous Bioactive Glasses (MBGs) for Potential Use in Bone Tissue Engineering Applications

“…The mobility of the HUVECs treated with the MBGs showed an increase at 24 h post-incubation ( Figure 12). These results are in good agreement with prior reports [20,52,53], and provide early evidence of the ability of Sr/Co-doped MBGs to potentially induce angiogenesis.…”

Osteogenic potential of sol–gel bioactive glasses containing manganese

“…Ions such as silver and gallium have demonstrated antibacterial properties [19,20], whereas cobalt can stimulate new blood vessel formation [21,22], similar effect observed in copper-doped glasses [23]. Those ions can be released during the glass dissolution process, improving the glass biological effect.…”

Multifunctional Copper-Containing Mesoporous Glass Nanoparticles as Antibacterial and Proangiogenic Agents for Chronic Wounds

“…Using other methods, Lin and co-workers (Lin et al, 2016) demonstrated that the growth of new vessels in the fibrous tissue of rat calvaria defects was promoted by copper ions released from a copper-containing bioactive glass scaffold. Romero-Sánchez et al (2018) explored the angiogenic effect exerted by the ionic products of both MBG and Cu-containing MBG. The proangiogenic effect of Cu-containing MBGs was confirmed by the increase in vessel number and thickness of the sub intestinal venous plexus (SIVP) in the in vivo zebrafish embryo assay.…”