Copper at low levels impairs memory of adult zebrafish ( Danio rerio ) and affects swimming performance of larvae

Acosta, Daiane da Silva; Danielle, Naissa Maria; Altenhofen, Stefani; Luzardo, Milene Dornelles; Costa, Patrícia Gomes; Bianchini, Adalto; Bonan, Carla Denise; Silva, Rosane Souza da; Dafre, Alcir Luiz

doi:10.1016/j.cbpc.2016.03.008

Cited by 41 publications

(14 citation statements)

References 65 publications

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“…Although several factors like a novel environment [74,75], age [76], dosage, or selective attentional functions [76,77] are associated with cholinergic neurotransmitter changes and might induce the measurement variations. However, in our study, we found the changes of cholinergic neurotransmitters and the dementia symptom observed in Fe 3 O 4 MNPs-exposed zebrafish brain is consistent with previous findings collected from other metal pollutions [78,79,80]. This result supports cholinergic neurotransmitters that can be used as a good and reliable indicator to report the memory deficiency induced by Fe 3 O 4 MNPs in zebrafish.…”

Section: Discussionsupporting

confidence: 92%

Ecotoxicity Assessment of Fe3O4 Magnetic Nanoparticle Exposure in Adult Zebrafish at an Environmental Pertinent Concentration by Behavioral and Biochemical Testing

et al. 2019

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Magnetic Nanoparticles (MNPs) are widely being investigated as novel promising multifunctional agents, specifically in the fields of development for theranostics, electronics, waste water treatment, cosmetics, and energy storage devices. Unique, superior, and indispensable properties of magnetization, heat transfer, and melting temperature make MNPs emerge in the field of therapeutics in future healthcare industries. However, MNPs ecotoxicity as well as behavioral toxicity is still unexplored. Ecotoxicity analysis may assist investigate MNPs uptake mechanism and its influence on bioavailability under a given set of environmental factors, which can be followed to investigate the biomagnification of MNPs in the environment and health risk possessed by them in an ecological food chain. In this study, we attempted to determine the behavioral changes in zebrafishes at low (1 ppm) or high (10 ppm) concentration levels of Fe3O4 MNPs. The synthesized Fe3O4 MNPs sized at 15 nm were characterized by the transmission electron microscope (TEM), the superconducting quantum interference device (SQUID) magnetometer, and the multiple behavior tests for novel tank, mirror biting, conspecific social interaction, shoaling, circadian rhythm, and short-term memory of zebrafish under MNPs chronic exposure were demonstrated. Low concentration MNP exposure did not trigger alteration for majority behavioral and biochemical tests in adult zebrafish. However, tight shoal groups were observed at a high concentration of MNPs exposure along with a modest reduction in fish exploratory behavior and a significant reduction in conspecific social interaction behavior. By using enzyme-linked immunosorbent assays (ELISA), we found a high dose of MNPs exposure significantly elevated cortisol, acetylcholine, and catalase levels while reducing serotonin, acetylcholine esterase, and dopamine levels in the brain. Our data demonstrates chronic MNPs exposure at an environmentally-relevant dose is relatively safe by supporting evidence from an array of behavioral and biochemical tests. This combinational approach using behavioral and biochemical tests would be helpful for understanding the MNPs association with anticipated colloids and particles effecting bioavailability and uptake into cells and organisms.

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Section: Discussionsupporting

confidence: 92%

Ecotoxicity Assessment of Fe3O4 Magnetic Nanoparticle Exposure in Adult Zebrafish at an Environmental Pertinent Concentration by Behavioral and Biochemical Testing

et al. 2019

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“…The main source of heavy metals originated from the runoff of the mine site, in addition to the floatation process used in preparing copper concentrates (Ali et al 2004). Copper plays crucial role in several integral parts of enzymes regarding respiration, collagen synthesis and to reduce free radicals (Acosta et al 2016). Organisms require a small amount of copper to regulate body metabolism.…”

Section: Introductionmentioning

confidence: 99%

In vivo and in vitro effects on cholinesterase of blood of Oreochromis mossambicus by copper

et al. 2019

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The present study is aimed to evaluate the effects of sub-acute toxicity testing of copper sulphate (CuSO 4), on behavioural, histological and biochemical changes of the Oreochromis mossambicus (black tilapia) blood tissues. The effects were assessed according to the previous results on sub-acute toxicity test after exposing fish to several concentrations (0.0, 2.5, 5.0, and 10.0 mg/L). The observations of scanning electron microscope, and transmission electron microscope studies revealed severe histopathological changes on the surface and the cellular changes in blood tissues, respectively. The morphological alterations in blood involved irregular structure of red blood cell and blood clot formation. CuSO 4 affected the biochemical alteration of the blood cholinesterase also known as serum cholinesterase (ChE). Blood ChE inhibited up to 80% of activity when exposed to 10.0 mg/L CuSO 4. The findings from this study can further improve the quality standards of aquaculture industry and the fundamental basis in selecting suitable strains among freshwater fish species to be used as bioindicator.

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“…It was expected that the less aggressive species would be displaced towards previously avoided contaminated habitats. Copper (Cu 2+ ) was selected as a test contaminant as it is an avoidable pollutant for fish (Moreira-Santos et al, 2008;Araújo et al, 2015) and produces toxic effects such as chemosensory deprivation (McIntyre et al, 2008), changes in gene expression (Craig et al, 2010), feeding inhibition (Abdel-Moneim et al, 2015), effects on the swimming performance of larvae (Acosta et al, 2016), morphological and metabolic alterations (Chatterjee et al, 2016), and changes in the surfaces of the gills (Fu et al, 2016). P. reticulata and D. rerio were selected as test organisms because they (i) are easy to cultivate, (ii) are widely used as model organisms in the fields of ecology, evolution, and ethology, and (iii) have already been shown to spatially avoid chemical contamination (Moreira-Santos et al, 2008;Silva et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Influence of interspecific interactions on avoidance response to contamination

Silva

Araújo

Marassi

et al. 2018

Science of The Total Environment

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An increasing number of studies have shown the ability of organisms to escape from toxic effects due to contamination, by moving spatially towards less contaminated habitats. However, this issue has been investigated in monospecific scenarios, without considering possible interactions between species during the contamination avoidance process. It is widely known that the spatial distribution of one species can be affected by another one, in different ways. Therefore, the main question addressed in the present study was as follows: Might interspecific interaction between the freshwater fish Danio rerio (zebrafish) and Poecilia reticulata (guppy) change their behavior patterns in terms of avoidance in the presence of a copper gradient? Zebrafish and guppies exposed to a copper gradient were tested for avoidance responses in a free-choice, non-forced, static, multi-compartmented exposure system, using two distinct approaches: (1) monospecific tests, in which only one species was exposed to the copper gradient, at two different population densities; and (2) multispecific tests, in which both species were tested simultaneously. In the control (with no copper) monospecific tests, both species were randomly distributed; however, in the control multispecific test, P. reticulata tended to aggregate. In the monospecific tests with a copper gradient, both species avoided copper in a similar way, with AC (concentration triggering avoidance in 50% of the exposed population) values between 15 and 18 μg·L, irrespective of the population density. However, in the multispecific tests, P. reticulata displaced D. rerio to previously avoided copper levels, consequently increasing the AC of D. rerio to 75 μg·L. This study shows the importance of understanding the interactions among species in contaminated areas, and the way that one species can prevent the avoidance behavior of another.

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Copper at low levels impairs memory of adult zebrafish ( Danio rerio ) and affects swimming performance of larvae

Cited by 41 publications

References 65 publications

Ecotoxicity Assessment of Fe3O4 Magnetic Nanoparticle Exposure in Adult Zebrafish at an Environmental Pertinent Concentration by Behavioral and Biochemical Testing

Ecotoxicity Assessment of Fe3O4 Magnetic Nanoparticle Exposure in Adult Zebrafish at an Environmental Pertinent Concentration by Behavioral and Biochemical Testing

In vivo and in vitro effects on cholinesterase of blood of Oreochromis mossambicus by copper

Influence of interspecific interactions on avoidance response to contamination

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