Copper and zinc concentrations in Bahía Blanca Estuary (Argentina), and their acute lethal effects on larvae of the crabChasmagnathus granulata

Ferrer, Laura; Andrade, Santiago; Contardi, Elena Teresa; Asteasuain, Raul Oscar; Marcovecchio, Jorge Eduardo

doi:10.3184/095422903782775271

Cited by 15 publications

(17 citation statements)

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“…The 96-h LC 50 values of copper for E. sinensis larvae were 0.16 and 0.21 mg L -1 at different growth stages, respectively. Copper toxicity for E. sinensis was of the same order of magnitude as for other crustacean larvae (Munshi et al 1996, Ferrer et al 2003, Amin and Comoglio 2010. The results obtained in this study showed that the lethal effects of copper were greater for zoea 1 larvae, suggesting that these larvae are more sensitive than megalopa larvae to water-borne copper.…”

Section: Discussionsupporting

confidence: 59%

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, Eriocheir sinensis (Decapoda: Brachyura) larvae

Sun

Zhu

et al. 2014

Sci. Mar.

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Summary:The lethal concentration of water-borne copper in Chinese mitten crab Eriocheir sinensis larvae was tested by exposing the animals to 0, 0.1, 0.2, 0.3, 0.5 and 0.8 mg Cu L −1 at 20°C for 96 h. The 96-h median lethal concentration (LC 50 ) and its corresponding 95% confident interval was estimated on zoea 1 larvae and megalopa larvae, respectively. Acute dissolved copper toxicity was higher for zoea 1 larvae (0.16 mg L -1 ) than for megalopa larvae (0.21 mg L -1 ). The antioxidant status, metallothionein-I mRNA expression and physiological response of the crab to copper toxicity was further investigated by exposing the megalopa larvae to 0, 0.08 and 0.16 mg Cu L −1 for 96 h. The superoxide dismutase activity, catalase activity, glutathione S-transferase (GST) activity and lipid peroxidation content of megalopa larvae increased concomitantly with the exposure time and copper concentration. MT-I mRNA expression levels were positively correlated with both the concentration and duration of copper exposure. The oxygen consumption and respiratory quotient of megalopa larvae in response to 0.16 mg L -1 copper were significantly higher than those in the control group after 96 h of exposure (P<0.05). The results of this study highlight the potential effects of copper as a common stressor in E. sinensis larvae. MT-I and GST appear to be suitable biomarkers of environmental copper exposure stress in E. sinensis larvae.Keywords: Eriocheir sinensis; copper toxicity; physiological; larvae; antioxidant enzyme; metallothionein.Efectos del cobre disuelto sobre la supervivencia, estado antioxidante, expresión de la metalotionina-I mRNA y la respuesta fisiológica de las larvas del cangrejo de Shangai, Eriocheir sinensis (Decapoda: Brachyura) Resumen: La concentración letal de cobre disuelto se determina mediante la exposición de larvas del cangrejo de Shangai (Eriocheir sinensis) a dosis de 0, 0.1, 0.2, 0.3, 0.5 y 0.8 mg Cu L -1 , a 20°C durante 96 h. La concentración letal media (LC 50 ) y su correspondiente intervalo de confianza del 95% fueron estimados en los estadios larvales zoea I y megalopa. La toxicidad aguda del cobre disuelto fue mayor sobre larvas de zoea I (0.16 mg L -1 ) que sobre las de megalopa (0.21 mg L -1 ). El estado antioxidante, la expresión del mRNA de la metalotionina-I y la respuesta fisiológica del cangrejo a la toxicidad del cobre fueron posteriormente investigados exponiendo las larvas de megalopa a dosis de 0, 0.08 y 0.16 mg Cu L -1 durante 96 h. La actividad de la superóxido dismutasa (SOD), de la catalasa (CAT), de la glutatión-S-transferasa (GST) y la peroxidación lipídica de las larvas de megalopa se incrementó en relación con el tiempo de exposición y la concentración de cobre. Los niveles de expresión de MT-1 mRNA se correlacionaron positivamente con la concentración de cobre y el tiempo de exposición. El consumo de oxígeno y la tasa respiratoria de las larvas de megalopa fueron significativamente más elevadas que las del grupo control (P<0.05) en respuesta a dosis de 0.16 mg Cu L -1 , du...

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Section: Discussionsupporting

confidence: 59%

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, Eriocheir sinensis (Decapoda: Brachyura) larvae

Sun

Zhu

et al. 2014

Sci. Mar.

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“…This reported value is also higher than those reported for other coastal zones of Argentina (i.e. 12 µg L -1 for Río de la Plata, Villar et al, 1999;and 4.65 µg L -1 for Bahía Blanca Estuary, Ferrer et al, 2003). It is therefore important to further the studies related to other aspects, such as moult, bioaccumulation and/or antioxidant defence responses of such a high heavy metal presence in the coastal zone of the Beagle Channel.…”

Section: Discussionmentioning

confidence: 56%

“…Copper toxicity for L. santolla was in the same order of magnitude as in other crustacean larvae ( Munshi et al, 1996;Ramachandran et al, 1997;Scelzo, 1997;López Greco et al, 2001;Ferrer et al, 2003). Copper was one order of magnitude more toxic than other metals assayed on the same species and larval stage, resulting in the following relative scale of acute lethal toxicity: Cu>Pb>Cd>Zn (Amin et al, 2003).…”

Section: Discussionmentioning

confidence: 78%

Effects of copper on the physiological responses of the commercial crab Lithodes santolla (Decapoda: Anomura) larvae

Amin¹,

Comoglio²

2010

Sci. Mar.

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SUMMARY: Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura) were analysed. The 96-h LC 50 was estimated, resulting in 298.5 µg L -1 . Groups of larvae were exposed to sublethal concentrations (40, 80 and 160 µg L -1 ) for 96 h. Oxygen consumption, ammonia excretion, O:N atomic ratio, lipid peroxidation (LPO) and body water content were measured. Oxygen consumption of treated groups (mean 46.92 ± 8.03 µg-atom O 2 h -1 mg -1 ) did not differ significantly with control. Ammonia excretion decreased by 60% at higher Cu concentration (1.61 ± 0.65 µg-atom N-NH 3 h -1 mg -1 ), leading to a 117% increase in the O:N ratio. LPO values during the exposure time were higher in all treatments than in the controls. The water content was significantly higher in treatments than in controls. The highest concentration assayed, which represents about 50% of 96-h LC 50 , had evident effects on the parameters analysed. The values of copper in water reported for the coastal zone of Ushuaia bay exceed the value established by the United States Environmental Protection Agency (USEPA) for ambient water quality criteria. Therefore, the results obtained in the present study are a contribution to the study of potential effects of copper as a common stressor in the first larval stage of this commercial species of the Beagle Channel.Keywords: crustacean larvae, oxygen consumption, ammonia excretion, O:N atomic ratio, lipid peroxidation, copper. RESUMEN: Impacto del cobre sobre las respuestas fisiológicas de la larva del cangrejo comercial Lithodes santoLLa (Decápoda: Anomura). -Los efectos de la toxicidad del cobre sobre la zoea I de Lithodes santolla (Decapoda: Anomura) fueron analizados. La CL50 96-h fue estimada en 298,5 µg L -1 . Grupos de larvas fueron expuestos a concentraciones subletales (40; 80 y 160 µg L -1 ) durante 96 horas. El consumo de oxígeno, excreción de amonio, relación atómica O:N, lipoperoxidación (LPO) y contenido de agua corporal fueron medidos. El consumo de oxígeno de los grupos tratados (media 46.92 ± 8.03 µg-átomo O 2 h -1 mg -1 ) no difirió significativamente del control. La excreción de amonio disminuyó un 60% en la mayor concentración de Cu (1.61 ± 0.65 µg-átomo N-NH 3 h -1 mg -1 ) produciendo un incremento del 117% en la tasa O:N. Los valores de LPO a lo largo de la exposición fueron mayores en todos los tratamientos respecto al control. El contenido de agua corporal fue mayor en los tratamientos que en el control. La mayor concentración ensayada, que representa alrededor del 50% de la CL50 96-h estimada produjo efectos evidentes sobre los parámetros analizados. Los valores de cobre en agua reportados para la zona costera de bahía Ushuaia exceden los establecidos por la Agencia de Protección Ambiental de Estados Unidos (USEPA) como criterio de calidad ambiental. Por esta razón, los resultados obtenidos en el presente estudio se convierten en una contribución sobre el impacto potencial del cobre como un estresor común durante el primer estadio larval de esta importante especie comercial ...

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“…Cd, Cu, Fe and Mn) in the waters of this system, working with either one or all the metals in the dissolved (Ferrer et al, 2000;Bott e et al, 2007Bott e et al, , 2008 or the particulate fraction (Fern andez Severini et al, 2013). Both compartments together were evaluated four times Ferrer et al, 2003;Bott e, 2005;Fern andez Severini et al, 2009) and only in one case (Fern andez Severini et al, 2009) the interaction between metals was analyzed, with samples taken from the subtidal area. In the present work, sampling was performed within intertidal areas, known as important sites within an estuary (Wood and Widdows, 2002;Feng et al, 2004;Bott e et al, 2007), which could be used as proxies for monitoring metal concentrations in a more immediate way.…”

Section: Introductionmentioning

confidence: 99%

Dissolved and particulate metals dynamics in a human impacted estuary from the SW Atlantic

Colla

Negrin

Marcovecchio

et al. 2015

Estuarine, Coastal and Shelf Science

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Copper and zinc concentrations in Bahía Blanca Estuary (Argentina), and their acute lethal effects on larvae of the crabChasmagnathus granulata

Cited by 15 publications

References 17 publications

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Effects of copper on the physiological responses of the commercial crab <i>Lithodes santolla</i> (Decapoda: Anomura) larvae

Dissolved and particulate metals dynamics in a human impacted estuary from the SW Atlantic

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