Copepods as References Species in Estuarine and Marine Waters

Kwok, Kevin W.H.; Souissi, Sami; Dur, Gaël; Won, Eun‐Ji; Lee, Jae‐Seong

doi:10.1016/b978-0-12-800949-9.00012-7

Cited by 23 publications

(16 citation statements)

References 161 publications

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“…This finding suggested that copepods and zooplankton were not responsible for the seeding of Vibrio on pelagic Sargassum. Furthermore, copepods, as heterotrophic bacteria grazers (Kwok et al, 2015) could be an important factor for the control of fast-growing gamma proteobacteria found in profiles P-Alt and P-Vib biofilm on Sargassum. Other environmental factors appeared to disfavour Vibrio.…”

Section: Discussionmentioning

confidence: 99%

In situ observations and modelling revealed environmental factors favouring occurrence of Vibrio in microbiome of the pelagic Sargassum responsible for strandings

Michotey

Blanfuné

Chevalier

et al. 2020

Science of The Total Environment

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Historically, pelagic Sargassum were only found in the Sargasso Sea. Since 2011, blooms were regularly observed in warmer water, further south. Their developments in Central Atlantic are associated with mass strandings on the coasts, causing important damages and potentially dispersion of new bacteria. Microbiomes associated with pelagic Sargassum were analysed at large scale in Central Atlantic and near Caribbean Islands with a focus on pathogenic bacteria. Vibrio appeared widely distributed among pelagic Sargassum microbiome of our samples with higher occurrence than previously found in Mexico Gulf. Six out the 16 Vibrio-OTUs (Operational Taxonomic Unit), representing 81.2 ± 13.1% of the sequences, felt in cluster containing pathogens. Among the four different microbial profiles of pelagic Sargassum microbiome, Vibrio attained about 2% in two profiles whereas it peaked, in the two others, at 6.5 and 26.8 % respectively, largely above the concentrations found in seawater surrounding raft (0.5%). In addition to sampling and measurements, we performed backward Lagrangian modelling of trajectories of rafts, and rebuilt the sampled rafts environmental history allowing us to estimate Sargassum growth rates along raft displacements. We found that Vibrio was favoured by high Sargassum growth rate and in situ ammonium and nitrite, modelled phosphate and nitrate concentrations, whereas zooplankters, benthic copepods, and calm wind (proxy of raft buoyancy near the sea surface) were less favourable for them. Relations between Vibrio and other main bacterial groups identified a competition with Alteromonas. According to forward Lagrangian tracking, part of rafts containing Vibrio could strand on the Caribbean coasts, however the strong decreases of modelled Sargassum growth rates along this displacement suggest unfavourable environment for Vibrio. For the conditions and areas observed, the sanitary risk seemed in consequence minor, but in other areas or conditions where high Sargassum growth rate occurred near coasts, it could be more important.

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Section: Discussionmentioning

confidence: 99%

In situ observations and modelling revealed environmental factors favouring occurrence of Vibrio in microbiome of the pelagic Sargassum responsible for strandings

Michotey

Blanfuné

Chevalier

et al. 2020

Science of The Total Environment

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“…In aquatic ecosystems, coastal areas are vulnerable to land pollution due to substances derived from industries, wastewater, farming, and tourism. Many studies have shown that aquatic organisms, whether in situ environments or cultured in the laboratory, can be used to evaluate pollution and to test ecotoxicological responses 19,20 . In particular, brackish water species have a wide range of adaptations to environmental physico-chemical conditions, such as salinity and temperature, and are considered suitable for monitoring and risk assessment of various pollutants originating from land and marine environments.…”

Section: Introductionmentioning

confidence: 99%

Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

Lee¹,

In²,

Kim³

et al. 2021

Preprint

Self Cite

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Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), a primary approach for evaluating gene expression, requires an appropriate normalization strategy to rule out variations in gene expression among samples. The best option is to use a reference gene whose expression level is stable across various experimental conditions to compare the mRNA levels of a target gene. However, there is limited information on how the reference gene is differentially expressed at different ages (growth) in small invertebrates with notable changes such as molting. In this study, expression profiles of nine candidate reference genes from the brackish water flea, Diaphanosoma celebensis, were evaluated under diverse exposure to toxicants and according to growth. As a result, four different algorithms showed similar stabilities of genes for chemical exposures in the case of limited conditions using the same developmental stage (e.g., adult), while the results according to age showed a significantly different pattern in suite of candidate reference genes. This affected the results of genes EcRA and GST, which are involved in development and detoxification mechanisms, respectively. Our finding is the first step towards establishing a standardized real-time qRT-PCR analysis of this environmentally important invertebrate that has potential for aquatic ecotoxicology, particularly in estuarine environments.

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“…They provide essential ecosystem services by supporting the maintenance of fish of economic importance. They are thus suitable model organisms for ecotoxicology and genomics studies [2,4,5] due to their primordial position in the trophic chain, copepod ubiquity, diversity, and capacity to transfer pollutants to higher trophic levels [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…The calanoid copepod E. affinis-a species with widespread presence in Northern Hemisphere estuaries [24]-was chosen as a model. E. affinis is a suitable test organism due to its small size, sexual dimorphism, short generation time, and ease of culturing in the laboratory [5,25]. This species has been used in several ecotoxicological studies to assess toxicant effects by (i) assessing whole-organism responses including survival, development, reproduction, and behaviour [26][27][28][29][30][31][32] and (ii) molecular investigations of gene expression [33,34].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of the Non-Targeted Crustacean Eurytemora affinis to the Endocrine Disruptor Tebufenozide: A Transcriptomic Approach

Arcanjo

Trémolet

Giusti-Petrucciani

et al. 2021

Genes

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Copepods are zooplanktonic crustaceans ubiquitously widespread in aquatic systems. Although they are not the target, copepods are exposed to a wide variety of pollutants such as insect growth regulators (IGRs). The aim of this study was to investigate the molecular response of a non-targeted organism, the copepod Eurytemora affinis, to an IGR. Adult males and females were exposed to two sub-lethal concentrations of tebufenozide (TEB). Our results indicate a sex-specific response with a higher sensitivity in males, potentially due to a differential activation of stress response pathways. In both sexes, exposure to TEB triggered similar pathways to those found in targeted species by modulating the transcription of early and late ecdysone responsive genes. Among them were genes involved in cuticle metabolism, muscle contraction, neurotransmission, and gametogenesis, whose mis-regulation could lead to moult, locomotor, and reproductive impairments. Furthermore, genes involved in epigenetic processes were found in both sexes, which highlights the potential impact of exposure to TEB on future generations. This work allows identification of (i) potential biomarkers of ecdysone agonists and (ii) further assessment of putative physiological responses to characterize the effects of TEB at higher biological levels. The present study reinforces the suitability of using E. affinis as an ecotoxicological model.

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Copepods as References Species in Estuarine and Marine Waters

Cited by 23 publications

References 161 publications

In situ observations and modelling revealed environmental factors favouring occurrence of Vibrio in microbiome of the pelagic Sargassum responsible for strandings

In situ observations and modelling revealed environmental factors favouring occurrence of Vibrio in microbiome of the pelagic Sargassum responsible for strandings

Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

Susceptibility of the Non-Targeted Crustacean Eurytemora affinis to the Endocrine Disruptor Tebufenozide: A Transcriptomic Approach

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