Copaiba oil suppresses inflammation in asthmatic lungs of BALB/c mice induced with ovalbumin

Caputo, Ludmila S.; Campos, Maria Inês C.; Dias, Herbert Júnior; Crotti, Antônio Eduardo Miller; Fajardo, Júlia Bertolini; Vanelli, Chislene Pereira; Presto, Álvaro C. D.; Alves, Maria Silvana; Aarestrup, Fernando Monteiro; Paula, Alessandro Vinícius de; Filho, Ademar A. Da Silva; Aarestrup, Beatriz Julião Vieira; Júnior, Olavo dos Santos Pereira; Corrêa, José Otávio do Amaral

doi:10.1016/j.intimp.2019.106177

Cited by 12 publications

(19 citation statements)

References 29 publications

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“…As confirmed by the histomorphometric and histopathological analyses, CO induced a significant decline in the number of inflammatories infiltrating in the lung tissue, such as in the parenchyma region. Furthermore, the findings show advantageous immunomodulatory effects of CO in asthmatic rats 58 …”

Section: Resultsmentioning

confidence: 74%

A mechanistic review on immunomodulatory effects of selective type two cannabinoid receptor β‐caryophyllene

Rahimi

Askari

2022

BioFactors

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β-Caryophyllene (BCP) is a plant-derived compound and occurs naturally in various foods and spices, including cinnamon, citrus, fruits, clove, curry, and pepper. BCP showed different pharmacological effects, such as antioxidant and antimicrobial properties. This article tried to gather updated knowledge of the anti-inflammatory, antioxidant, and immunomodulatory effects of BCP and searched using various databases and appropriate keywords until April 2022. Several studies showed that the anti-inflammatory effects of BCP are mainly provided through cannabinoid receptor 2 (CB 2 ) receptor activation and the peroxisome proliferator-activated receptor (PPAR) γ pathway. It has also been demonstrated that BCP suppresses both protein and mRNA expression levels of interleukin (IL)-6 and reduces relevant proinflammatory cytokines but increases the anti-inflammatory cytokine IL-13. Previous results indicated that the antioxidant effects of β-caryophyllene were suggested through different pathways, including activation of nuclear factor erythroid 2-related factor-2 (Nrf2)/heme oxygenase-1 (HO-1)/antioxidant axis and inhibition of the HMG-CoA reductase activity, and oxidative stress biomarkers levels. Furthermore, various results showed immunomodulatory effects of BCP through inhibiting microglial cells, CD 4+ and CD 8+ T lymphocytes, modulated Th 1 /T reg immune balance through the activation of the CB 2 receptor, and reducing mitogenactivated protein kinases (p38MAPK) and NF-kB activation and increased ionized calcium-binding adaptor molecule-1 (Iba-1) and IL-1β.

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Section: Resultsmentioning

confidence: 74%

A mechanistic review on immunomodulatory effects of selective type two cannabinoid receptor β‐caryophyllene

Rahimi

Askari

2022

BioFactors

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“…Finally, it is important to highlight that, even with these observed variations, the main bioactive molecules of the Copaifera genus are presented, assuring their identity, as they have been previously studied by our research group. In addition, the biological effect of these identified compounds suggests the use of this oil for the treatment of inflammatory disorders, as described by Caputo et al (2020) in a model of pulmonary inflammation and by Ghizoni et al (2017) in a model of arthritis [ 24 , 25 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

Copaiba Oil-Loaded Polymeric Nanocapsules: Production and In Vitro Biosafety Evaluation on Lung Cells as a Pre-Formulation Step to Produce Phytotherapeutic Medicine

et al. 2023

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Copaiba oil has been largely used due to its therapeutic properties. Nanocapsules were revealed to be a great nanosystem to carry natural oils due to their ability to improve the bioaccessibility and the bioavailability of lipophilic compounds. The aim of this study was to produce and characterize copaiba oil nanocapsules (CopNc) and to evaluate their hemocompatibility, cytotoxicity, and genotoxicity. Copaiba oil was chemically characterized by GC-MS and FTIR. CopNc was produced using the nanoprecipitation method. The physicochemical stability, toxicity, and biocompatibility of the systems, in vitro, were then evaluated. Β-bisabolene, cis-α-bergamotene, caryophyllene, and caryophyllene oxide were identified as the major copaiba oil components. CopNc showed a particle size of 215 ± 10 nm, a polydispersity index of 0.15 ± 0.01, and a zeta potential of −18 ± 1. These parameters remained unchanged over 30 days at 25 ± 2 °C. The encapsulation efficiency of CopNc was 54 ± 2%. CopNc neither induced hemolysis in erythrocytes, nor cytotoxic and genotoxic in lung cells at the range of concentrations from 50 to 200 μg·mL−1. In conclusion, CopNc showed suitable stability and physicochemical properties. Moreover, this formulation presented a remarkable safety profile on lung cells. These results may pave the way to further use CopNc for the development of phytotherapeutic medicine intended for pulmonary delivery of copaiba oil.

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“…The results of the pulmonary airspace area (white) were expressed as a percentage [ 24 ]. This involved defining the scale of the images (200 µm) to allow for the measurement of the distance from the airspaces or pulmonary parenchyma (depending on what is to be analyzed), converting them later into 8-bit (in gray-scale) and applying threshold to the images, using the black and white (B&W) tool to limit the intensity of the colors in the histopathological samples in front of the areas of interest (white tone for the airspace, including alveoli, alveolar, and bronchioles; black for the parenchyma pulmonary).…”

Section: Methodsmentioning

confidence: 99%

Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice

et al. 2022

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Diets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 μL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions.

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Copaiba oil suppresses inflammation in asthmatic lungs of BALB/c mice induced with ovalbumin

Cited by 12 publications

References 29 publications

A mechanistic review on immunomodulatory effects of selective type two cannabinoid receptor β‐caryophyllene

A mechanistic review on immunomodulatory effects of selective type two cannabinoid receptor β‐caryophyllene

Copaiba Oil-Loaded Polymeric Nanocapsules: Production and In Vitro Biosafety Evaluation on Lung Cells as a Pre-Formulation Step to Produce Phytotherapeutic Medicine

Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice

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