Coordination-based self-assembled capsules (SACs) for protein, CRISPR–Cas9, DNA and RNA delivery

Alimi, Lukman O.; Alyami, Mram Z.; Chand, Santanu; Baslyman, Walaa; Khashab, Niveen M.

doi:10.1039/d0sc05975g

Cited by 30 publications

(22 citation statements)

References 146 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to this principle, many MOF‐based gene therapies have integrated the MOF's inherent response to pH and enhanced endosomal escape ability to protect gene editing tools from being degraded by enzymes, thereby enhancing the efficiency of gene therapy. [ 53 ]…”

Section: Subcellular Organelles‐targeted Mof Therapymentioning

confidence: 99%

Biomedical Applications of Metal–Organic Frameworks at the Subcellular Level

Xue

Liu

Yong

et al. 2021

Advanced NanoBiomed Research

View full text Add to dashboard Cite

Organelles, or subcellular structures, are the fundamental functional units in almost all eukaryotic cells. Consequently, they play a critical role in the development of various biomedical applications, such as targeted drug delivery, biosensing, imaging, and biomimetics. Many efforts are devoted to developing nanosystems that can target specific organelles in a controlled manner. A series of nanomaterials with high porosity, stability, and easy‐to‐tailor properties, named metal–organic frameworks (MOFs), have shown to be a promising new class of nanocarriers and bioprotective exoskeletons for biomedical and biocatalytical platforms at the subcellular level. Herein, the recent state‐of‐the‐art progress of MOF nanomaterials for bioapplications at the subcellular level is highlighted. In the first section, MOF‐derived biomimetics organelles and subcellular structures are highlighted. Then, the strategies of organelle‐targeted MOF therapy and biosensing are illustrated. Next, MOF‐based biopreservation of organelles is introduced. Finally, a personal perspective about the challenges and future innovative designs is discussed.

show abstract

Section: Subcellular Organelles‐targeted Mof Therapymentioning

confidence: 99%

Biomedical Applications of Metal–Organic Frameworks at the Subcellular Level

Xue

Liu

Yong

et al. 2021

Advanced NanoBiomed Research

View full text Add to dashboard Cite

show abstract

“…42−45 MOFs have large specific surface areas with tunable porosity and can respond to internal and external stimuli. 43 Specifically, they can efficiently encapsulate and load cargos ranging from small molecules to biomacromolecules while protecting them from enzymatic degradation. There are a few published reports on MOF coated magnetic nanostructures for catalytic applications, but no investigations on the therapeutic applications of such systems have been reported so far.…”

Section: ■ Introductionmentioning

confidence: 99%

Iron Nanowires Coated with Metal–Organic Frameworks for Cell-Type-Specific Multimodal Therapy

Alyami

Patel

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Targeted therapy has the potential to revolutionize medical approaches to bring us closer to an effective personalized therapy model. In this work, we developed homologous-targeted magnetic nanowires coated with biologics-loaded zeolitic imidazolate frameworks and cellular membranes (ZNWs) to provide an increased therapeutic potential both in vitro and in vivo. In vitro, the uncoated ZNWs combined with laser treatment reduced cancer cell viability by 34.6%, whereas the coated ZNWs combined with laser treatment reduced cancer cell viability by 45.91%. In vivo, ZNWs accumulated selectively in MCF-7 cancer cells and maintained a high intensity for up to 120 h, which reduced tumor growth significantly. Combining gene and tumor ablation treatment with the homologous targeting ability of ZNWs resulted in about 73% cell death. We believe that using such an assembled coating to camouflage delivery vehicles has effective outcomes in terms of improved targeting and therapeutic efficiency and should be studied further for medical translation.

show abstract

“…This limits the substrate range and concentration that can be tolerated by the enzyme and their structural stability at elevated temperatures. , Thus, there is significant interest in methodologies that enhance tolerance of enzymes to high substrate concentration and challenging conditions . Immobilisation of enzymes on a solid support is one strategy that has been explored to protect enzymes from harsh environments while also facilitating reusability. − One class of materials that has recently shown promise for enzyme immobilization is metal–organic frameworks (MOFs). − Enzyme@MOF biocomposites can be synthesized via infiltrating biomacromolecules into the framework pores, surface adsorption, covalent attachment, and “one-pot” encapsulation of enzymes within MOF crystals . The latter approach has shown a remarkable capacity to protect enzymes from external stressors. , It is proposed that this is due to the tight encapsulation of the enzyme within pore voids possessing dimensions that restrict protein unfolding and loss of native activity .…”

Section: Introductionmentioning

confidence: 99%

Influence of the Synthesis and Storage Conditions on the Activity of Candida antarctica Lipase B ZIF-8 Biocomposites

Maddigan

Linder‐Patton

Falcaro

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The biomimetic mineralization of zeolitic imidazolate framework-8 (ZIF-8) has been reported as a strategy for enzyme immobilization, enabling the heterogenization and protection of biomacromolecules. Here, we report the preparation of different Candida antarctica lipase B biocomposites (CALB@ ZIF-8) formed by altering the concentrations of Zn 2+ and 2methylimidazole (2-mIM). The influence of synthetic conditions on the catalytic activity of the lipase CALB was examined by hydrolysis and transesterification assays in aqueous and organic media, respectively. We demonstrated that for both reactions, activity was retained for the biocomposites formed at low Zn 2+ /2-mIM ratios but notably almost entirely lost when the ligand concentration used to form the biocomposites was increased. Additionally, phosphate buffer could regenerate the activity of larger particles by degrading the crystal surfaces and releasing encapsulated CALB into solution. Transesterification reactions using CALB@ZIF-8 biocomposites were undertaken in 100% hexane, giving rise to enhanced CALB activity relative to the free enzyme. These observations highlight the fundamental importance of synthetic protocols and operating parameters for developing enzyme@ MOF biocomposites with improved activity in challenging conditions.

show abstract

Coordination-based self-assembled capsules (SACs) for protein, CRISPR–Cas9, DNA and RNA delivery

Abstract: SACs can be efficiently used to load biologics such as proteins, CRISPR–Cas9, DNA and RNA and release them on-demand.

Cited by 30 publications

References 146 publications

Biomedical Applications of Metal–Organic Frameworks at the Subcellular Level

Biomedical Applications of Metal–Organic Frameworks at the Subcellular Level

Iron Nanowires Coated with Metal–Organic Frameworks for Cell-Type-Specific Multimodal Therapy

Influence of the Synthesis and Storage Conditions on the Activity of Candida antarctica Lipase B ZIF-8 Biocomposites

Contact Info

Product

Resources

About