Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding

Abstract

Epithelial wounding causes waves of actomyosin flow and apical cell constriction that are dependent on calcium signaling and actin filament severing.

Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair

“…Wound closure during axis elongation is driven by the apical constriction of the wounded cells in combination with the assembly of an actomyosin purse string by the cells adjacent to the wound (Fernandez-Gonzalez and Zallen, 2013). At later stages of development, deformation of cells around the wound drives calcium influx through mechanically gated channels and promotes the assembly of the actomyosin purse string (Antunes et al, 2013). However, little is known about the cell morphology changes that accompany wound repair during axis elongation.…”

Apical constriction: themes and variations on a cellular mechanism driving morphogenesis

“…The mechanical induction of myosin assembly could occur through regulation of the G protein-coupled receptor pathway that activates RhoA, with mechanical stimulation increasing plasma membrane tension and decreasing endocytic downregulation of the receptor, thus elevating pathway activity (Pouille et al, 2009). Recent studies of wound healing demonstrated that apical constriction in response to wounding is associated with cytoplasmic calcium accumulation through a stretch-activated calcium channel (Antunes et al, 2013). During Drosophila dorsal closure, calcium signaling has been shown to promote contractility, and two ion channels were identified as being required for embryos to regulate force generation after laser ablation (Hunter et al, 2014).…”

“…Apical constriction of individual cells can contribute to cell ingression from epithelial tissues, sometimes as a step in an epithelialmesenchymal transition (EMT) (Anstrom, 1992;Nance and Priess, 2002;Harrell and Goldstein, 2011;Williams et al, 2012). Apical constriction is also associated with the extrusion of apoptotic or delaminating cells (Toyama et al, 2008;Slattum et al, 2009;Marinari et al, 2012) and wound contraction and healing (Davidson et al, 2002;Antunes et al, 2013). Thus, apical constriction remodels epithelia in a variety of ways to achieve proper tissue shape and structure.…”

Self-Similarity, Distortion Waves, and the Essence of Morphogenesis: A Generalized View of Color Pattern Formation in Butterfly Wings

“…Fortunately, mechanobiology is an expanding interdisciplinary field between biology and physics (Iskratsch et al 2014). Changes in the mechanical property of a cellular sheet may be caused by physical damage and subsequent wound-healing processes (Antunes et al 2013) and by cell death (Teng and Toyama 2011;Toyama et al 2008) in addition to cellular size and shape changes. …”