Coordinated Transcriptional Regulation by the UV-B Photoreceptor and Multiple Transcription Factors for Plant UV-B Responses

Qian, Changli; Chen, Zhiren; Liu, Qing; Mao, Weiwei; Chen, Yanling; Wei, Tang; Liu, Yan; Han, Jiupan; Ouyang, Xinhao; Huang, Xi

doi:10.1016/j.molp.2020.02.015

Cited by 87 publications

(69 citation statements)

References 56 publications

(69 reference statements)

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“…We speculate that these genes may be related to the developmental regulation and ecological adaptability of the two subpopulations. For instance, UV-B light and the abscisic acid receptor PYL8 can regulate cotyledon root development and stress adaptation by MYB13 interaction (Zhao et al, 2014b;Qian et al, 2020). The abscisic acid receptor PYL9 improves drought resistance by regulating leaf senescence (Zhao et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Revealing Distinctions in Genetic Diversity and Adaptive Evolution Between Two Varieties of Camellia sinensis by Whole-Genome Resequencing

Zhao

et al. 2020

Front. Plant Sci.

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Camellia sinensis var. sinensis (CSS) and C. sinensis var. assamica (CSA) are the two most economically important tea varieties. They have different characteristics and geographical distribution. Their genetic diversity and differentiation are unclear. Here, we identified 18,903,625 single nucleotide polymorphisms (SNPs) and 7,314,133 insertion–deletion mutations (indels) by whole-genome resequencing of 30 cultivated and three wild related species. Population structure and phylogenetic tree analyses divided the cultivated accessions into CSS and CSA containing 6,440,419 and 6,176,510 unique variations, respectively. The CSS subgroup possessed higher genetic diversity and was enriched for rare alleles. The CSA subgroup had more non-synonymous mutations and might have experienced a greater degree of balancing selection. The evolution rate (dN/dS) and KEGG enrichment indicated that genes involved in the synthesis and metabolism of flavor substances were positively selected in both CSS and CSA subpopulations. However, there are extensive genome differentiation regions (2959 bins and approximately 148 M in size) between the two subgroups. Compared with CSA (141 selected regions containing 124 genes), the CSS subgroup (830 selected regions containing 687 genes) displayed more selection regions potentially related to environmental adaptability. Fifty-three pairs of polymorphic indel markers were developed. Some markers were located in hormone-related genes with distinct alleles in the two cultivated subgroups. These identified variations and selected regions provide clues for the differentiation and adaptive evolution of tea varieties. The newly developed indel markers will be valuable in further genetic research on tea plants.

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Section: Discussionmentioning

confidence: 99%

Revealing Distinctions in Genetic Diversity and Adaptive Evolution Between Two Varieties of Camellia sinensis by Whole-Genome Resequencing

Zhao

et al. 2020

Front. Plant Sci.

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“…UV-B is sensed by the photoreceptor UVR8, which activates the transcription factor HY5; then, HY5 up-regulates the expression of the transcription factors that control the biosynthesis of flavonoids and anthocyanins (Brown et al, 2005;Stracke et al, 2010;Tohge et al, 2011;Shin et al, 2013). In addition, it has been shown that UVR8 binds directly to the transcription factor MYB13 regulating flavonoid biosynthesis in a UV-B-dependent manner (Qian et al, 2020). Among the members in MBW complexes, at least MYB12, MYB111, MYB75, MYB13 and GL3 have been shown to be induced by UV-B (Brown et al, 2005;Stracke et al, 2010;Tohge et al, 2011;Yan et al, 2012;Qian et al, 2020).…”

Section: Narrowband Uv-b Irradiation Does Not Induce Flavonoid Biosynmentioning

confidence: 99%

“…In addition, it has been shown that UVR8 binds directly to the transcription factor MYB13 regulating flavonoid biosynthesis in a UV-B-dependent manner (Qian et al, 2020). Among the members in MBW complexes, at least MYB12, MYB111, MYB75, MYB13 and GL3 have been shown to be induced by UV-B (Brown et al, 2005;Stracke et al, 2010;Tohge et al, 2011;Yan et al, 2012;Qian et al, 2020). In this study HY5, MYBL2, and TTG1 were significantly up-regulated, and HYH and MYB12 also tended to be up-regulated by both 280 and 310 nm UV-LED irradiation.…”

Section: Narrowband Uv-b Irradiation Does Not Induce Flavonoid Biosynmentioning

confidence: 99%

“…Expression of these enzymatic genes is regulated by several classes of transcription factors, such as MYB-bHLH-WDR (MBW) complexes (Broun 2005;Xu et al, 2014Xu et al, , 2015Ma and Constabel 2019), and coregulatory Mediator complexes (Bonawitz et al, 2012;Wang et al, 2020). Flavonoid biosynthesis is up-regulated under UV-B through regulation of these transcription factors mediated by UVR8 signaling (Jenkins 2014;Ma and Constabel 2019;Qian et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome and metabolome analyses revealed that narrowband 280 and 310 nm UV-B induce distinctive responses in Arabidopsis

Tsurumoto

Fujikawa²,

Ohta

et al. 2021

Preprint

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In plants, the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) perceives UV-B and induces UV-B responses including synthesis of UV-B absorbing phenolic compounds such as anthocyanins. UVR8 absorbs a range of UV-B (260–335 nm). However, the responsiveness of plants to each UV-B wavelength has not been intensively studied so far. Here, we performed transcriptome and metabolome analyses of Arabidopsis using UV light emitting diodes (LEDs) with peak wavelengths of 280 and 310 nm to investigate the differences in the wavelength-specific UV-B responses. Irradiation with both UV-LEDs induced gene expression of the transcription factor ELONGATED HYPOCOTYL 5 (HY5), which has a central role in the UVR8 signaling pathway. However, the overall transcriptomic and metabolic responses to 280 and 310 nm UV-LED irradiation were different. Most of the known UV-B-responsive genes, such as salicylic acid, jasmonic acid, and defense-related genes, responded only to 280 nm UV-LED irradiation. Lipids, polyamines and organic acids were the metabolites most affected by 280 nm UV-LED irradiation, whereas the effect of 310 nm UV-LED irradiation on the metabolome was considerably less. Enzymatic genes involved in the phenylpropanoid pathway upstream in anthocyanin biosynthesis were up-regulated only by 280 nm UV-LED irradiation. On the other hand, no enzymatic genes downstream in anthocyanin biosynthesis were induced by the UV-LEDs, but rather, they were down-regulated by 310 nm UV-LED irradiation. These results revealed that the responsivenesses of Arabidopsis to 280 and 310 nm UV-B were significantly different, suggesting that UV-B signaling is mediated by more complex pathways than the current model.

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“…The COP1 substrate ELONGATED HYPOCOTYL 5 (HY5), a crucial positive regulator of light signaling (Osterlund et al, 2000), is induced transcriptionally and stabilized post-transcriptionally, and is necessary for the regulation of most UVR8-induced genes (Ulm et al, 2004;Brown et al, 2005;Oravecz et al, 2006;Favory et al, 2009;Huang et al, 2012;Binkert et al, 2014). Additionally, UVR8 regulates the stability or DNA-binding activity of a set of transcription factors affecting UV-B responses (Hayes et al, 2014;Liang et al, 2018;Yang et al, 2018;Sharma et al, 2019;Qian et al, 2020;Tavridou et al, 2020a;Tavridou et al, 2020b;Yang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

Podolec

Lau

Wagnon

et al. 2020

Preprint

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The plant UV-B photoreceptor UVR8 plays an important role in UV-B acclimation and survival. UV-B absorption by homodimeric UVR8 induces its monomerization and interaction with the E3 ubiquitin ligase COP1, leading ultimately to gene expression changes. UVR8 is inactivated through redimerization, facilitated by RUP1 and RUP2. Here, we describe a novel semi-dominant, hyperactive allele, namely uvr8-17D, that harbors a glycine-101 to serine mutation. UVR8G101S-overexpression led to weak constitutive photomorphogenesis and extreme UV-B responsiveness. UVR8G101S was observed to be predominantly monomeric in vivo and, once activated by UV-B, was not efficiently inactivated. Analysis of a UVR8G101S crystal structure revealed the distortion of a loop region normally involved in stabilization of the UVR8 homodimer. Plants expressing a UVR8 variant combining G101S with the previously described W285A mutation exhibited robust constitutive photomorphogenesis. This work provides further insight into UVR8 activation and inactivation mechanisms, and describes a genetic tool for the manipulation of photomorphogenic responses.

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Coordinated Transcriptional Regulation by the UV-B Photoreceptor and Multiple Transcription Factors for Plant UV-B Responses

Cited by 87 publications

References 56 publications

Revealing Distinctions in Genetic Diversity and Adaptive Evolution Between Two Varieties of Camellia sinensis by Whole-Genome Resequencing

Revealing Distinctions in Genetic Diversity and Adaptive Evolution Between Two Varieties of Camellia sinensis by Whole-Genome Resequencing

Transcriptome and metabolome analyses revealed that narrowband 280 and 310 nm UV-B induce distinctive responses in Arabidopsis

A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

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