Coordinated control of the type IV pili and c‐di‐GMP‐dependent antifungal antibiotic production in <i>L</i><i>ysobacter</i> by the response regulator PilR

Xu, Kangwen; Shen, Danyu; Yang, Nianfu; Chou, Shan‐Ho; Gomelsky, Mark; Qian, Guoliang

doi:10.1111/mpp.13046

Cited by 15 publications

(14 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two-component systems have gained considerable attention due to their role in modulating a plethora of virulence mechanisms and multidrug resistance. In A. baumannii these systems include: BfmRS, which is involved in fitness, capsule production and biofilm formation; AdeRS, which regulates an efflux pump associated with antibiotic resistance; GacSA, which is involved with regulating the phenyl acetic acid catabolic pathway as well as attenuated virulence in a mouse infection model; the BaeSR system, which modulates efflux pumps and has been implicated in cross talk with the AdeRS systems, and the PmrAB system, which is involved in colistin resistance [ 4 , 5 , 26 , 66 , 78 ]. In this study the diverse nature of these systems was explored through the uncharacterized TCS, StkSR.…”

Section: Discussionmentioning

confidence: 99%

“…Comparative bioinformatics analysis of the genes encoding potential response regulator proteins in the WT 04117201 A. baumannii strain identified five TCS that are absent from the avirulent A. baumannii strain SDF and therefore are hypothesized to affect virulence potential ( Table 2 ). These systems included two orphan response regulators, NasT and RsbU, and two previously characterized response regulators, AdeR, which is associated with efflux pump regulation, and BarA, coordinating the control of type IV pili [ 24 , 66 ]. The novel hybrid TCS named here as StkSR was chosen for further phenotypic investigation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The StkSR Two-Component System Influences Colistin Resistance in Acinetobacter baumannii

et al. 2022

View full text Add to dashboard Cite

Acinetobacter baumannii is an opportunistic human pathogen responsible for numerous severe nosocomial infections. Genome analysis on the A. baumannii clinical isolate 04117201 revealed the presence of 13 two-component signal transduction systems (TCS). Of these, we examined the putative TCS named here as StkSR. The stkR response regulator was deleted via homologous recombination and its progeny, ΔstkR, was phenotypically characterized. Antibiogram analyses of ΔstkR cells revealed a two-fold increase in resistance to the clinically relevant polymyxins, colistin and polymyxin B, compared to wildtype. PAGE-separation of silver stained purified lipooligosaccharide isolated from ΔstkR and wildtype cells ruled out the complete loss of lipooligosaccharide as the mechanism of colistin resistance identified for ΔstkR. Hydrophobicity analysis identified a phenotypical change of the bacterial cells when exposed to colistin. Transcriptional profiling revealed a significant up-regulation of the pmrCAB operon in ΔstkR compared to the parent, associating these two TCS and colistin resistance. These results reveal that there are multiple levels of regulation affecting colistin resistance; the suggested ‘cross-talk’ between the StkSR and PmrAB two-component systems highlights the complexity of these systems.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The StkSR Two-Component System Influences Colistin Resistance in Acinetobacter baumannii

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Most environmental and intracellular factors that control cellular c-di-GMP levels in L. enzymogenes remain to be deciphered, however, enzymes important for the c-di-GMP-dependent HSAF production have been identified (Ren et al, 2020). One DGC, LchD, has already been mentioned as the subject of PilR repression (Xu et al, 2021b). Clp interacts with LchD directly and stimulates its DGC activity (Fig.…”

Section: Regulation Of Hsaf Biosynthesismentioning

confidence: 99%

“…The gene expression and promoter–reporter analyses revealed that HSAF production is controlled primarily at the gene expression level (Chen et al ., 2017). Six transcription regulators that affect the expression of the HSAF biosynthesis operon have been identified so far, including five positive regulators, PilR, Clp, LysR, LarR and CdgL, and one negative regulator, LetR (Wang et al ., 2014; Chen et al ., 2017; Su et al ., 2017; Wang et al ., 2017; Su et al ., 2018; Xu et al ., 2018; Han et al ., 2020; Xu et al ., 2021b). Clp, LysR, LarR and LetR are transcription factors that directly bind to the promoter of the HSAF biosynthesis operson (Fig.…”

Section: Hsaf the Long‐range Antifungal Weapon Of L Enzymogenesmentioning

confidence: 99%

“…C‐di‐GMP is synthesized via the condensation of two GTP molecules by DGCs with the GGDEF catalytic domains (Paul et al ., 2004; Ryjenkov et al ., 2005), while it is hydrolyzed primarily by specific phosphodiesterases (PDEs) contacting either EAL or HD‐GYP catalytic domains (Schmidt et al ., 2005; Bellini et al ., 2014). As we discuss below, c‐di‐GMP plays a central role in suppressing HSAF production (Xu et al ., 2021b). In fact, the c‐di‐GMP‐dependent regulation of the production of antimicrobial compounds is common to many bacteria and is discussed in recent reviews (Liang, 2015; Qian et al ., 2020).…”

Section: Hsaf the Long‐range Antifungal Weapon Of L Enzymogenesmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal weapons of Lysobacter, a mighty biocontrol agent

Lin

Shen

et al. 2021

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Bacteria interact with fungi in a variety of ways to inhibit fungal growth, while the underlying mechanisms remain only partially characterized. The plantbeneficial Bacillus and Pseudomonas species are well-known antifungal biocontrol agents, whereas Lysobacter are far less studied. Members of Lysobacter are easy to grow in fermenters and are safe to humans, animals and plants. These environmentally ubiquitous bacteria use a diverse arsenal of weapons to prey on other microorganisms, including fungi and oomycetes. The small molecular toxins secreted by Lysobacter represent long-range weapons effective against filamentous fungi. The secreted hydrolytic enzymes act as intermediaterange weapons against non-filamentous fungi. The contact-dependent killing devices are proposed to work as short-range weapons. We describe here the structure, biosynthetic pathway, action mode and applications of one of the best-characterized longrange weapons, the heat-stable antifungal factor (HSAF) produced by Lysobacter enzymogenes. We discuss how the flagellar type III secretion system has evolved into an enzyme secretion machine for the intermediate-range antifungal weapons. We highlight an intricate mechanism coordinating the production of the long-range weapon, HSAF and the proposed contact-dependent killing device, type VI secretion system. We also overview the regulatory mechanisms of HSAF production involving specific transcription factors and the bacterial second messenger c-di-GMP.

show abstract

The role of bacterial signaling networks in antibiotics response and resistance regulation

Feng

Wang

2022

Mar Life Sci Technol

View full text Add to dashboard Cite

Excessive use of antibiotics poses a threat to public health and the environment. In ecosystems, such as the marine environment, antibiotic contamination has led to an increase in bacterial resistance. Therefore, the study of bacterial response to antibiotics and the regulation of resistance formation have become an important research field. Traditionally, the processes related to antibiotic responses and resistance regulation have mainly included the activation of efflux pumps, mutation of antibiotic targets, production of biofilms, and production of inactivated or passivation enzymes. In recent years, studies have shown that bacterial signaling networks can affect antibiotic responses and resistance regulation. Signaling systems mostly alter resistance by regulating biofilms, efflux pumps, and mobile genetic elements. Here we provide an overview of how bacterial intraspecific and interspecific signaling networks affect the response to environmental antibiotics. In doing so, this review provides theoretical support for inhibiting bacterial antibiotic resistance and alleviating health and ecological problems caused by antibiotic contamination.

show abstract

Coordinated control of the type IV pili and c‐di‐GMP‐dependent antifungal antibiotic production in Lysobacter by the response regulator PilR

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 15 publications

References 59 publications

The StkSR Two-Component System Influences Colistin Resistance in Acinetobacter baumannii

The StkSR Two-Component System Influences Colistin Resistance in Acinetobacter baumannii

Antifungal weapons of Lysobacter, a mighty biocontrol agent

The role of bacterial signaling networks in antibiotics response and resistance regulation

Contact Info

Product

Resources

About