Coordinated control of neuronal differentiation and wiring by sustained transcription factors

Özel, Mehmet Neset; Gibbs, Claudia Skok; Holguera, Isabel; Soliman, Mennah; Bonneau, Richard; Desplan, Claude

doi:10.1126/science.add1884

Cited by 20 publications

(12 citation statements)

References 67 publications

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“…Acj6 was expressed in the central brain and vtIPC LCNs, but not tOPC LCNs (Figure 2I, 6A, C, D, 7 E, F). This lack of common TF markers suggests that shared anatomical features of LCNs have likely evolved independently in different neurogenic pools downstream of different identity TFs (Özel et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

Morphological and functional convergence of visual projections neurons from diverse neurogenic origins inDrosophila

El-Danaf,

Kapuralin,

Rajesh

et al. 2024

Preprint

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The Drosophila visual system is a powerful model to study the development of neural circuits. Projection neurons that relay visual information from the lobula part of the optic lobe to the central brain (the lobula columnar neurons-LCNs), are thought to encode different visual features relevant to natural behavior. There are ~20 classes of LCNs whose projections form highly specific, non-overlapping synaptic domains in the brain called optic glomeruli. Although functional investigations of several LCN circuits have been carried out, very little is known about their developmental origin and the stem cell lineages that generate the LCN subtypes. To address their origin, we used single-cell mRNA sequencing to define the transcriptome of each LCN subtype and identified driver lines that are expressed in specific LCNs throughout development. We show that LCNs originate from neural stem cells in four distinct regions in the fly brain that exhibit different modes of neurogenesis, including the ventral and dorsal tips of the outer proliferation center (tOPC), the ventral tips of the inner proliferation center (vtIPC) and the central brain (CB). This convergence of similar neurons illustrates the complexity of generating neuronal diversity in the brain and likely reflects the evolutionary origin of each LCN subtype that detects a highly specific visual feature and influence behaviors that might be specific to each species.

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Section: Resultsmentioning

confidence: 99%

Morphological and functional convergence of visual projections neurons from diverse neurogenic origins inDrosophila

El-Danaf,

Kapuralin,

Rajesh

et al. 2024

Preprint

Self Cite

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“…Furthermore, combining sc-RNA-seq with other "-omics" approaches, such as single cell ATAC-seq chromatin profiling, has provided major new knowledge about the GRNs for Drosophila eye and optic lobe development, and predictive power to model and test the regulatory logic underling gene expression. [106][107][108] Applying these technologies to the developing eyes and visual systems of other insects, as recently carried out for honeybees, [109] will provide a framework to characterize how these genetic programmes evolve to produce visual systems with different morphologies, information processing, and functions. Second, overcoming challenges to culturing other insects in the lab is crucial to understand, compare, and manipulate eye development and has recently successfully been carried out for mayflies for the first time [24] (Box 2).…”

Section: Discussionmentioning

confidence: 99%

Looking across the gap: Understanding the evolution of eyes and vision among insects

Kittelmann,

McGregor

2024

BioEssays

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The compound eyes of insects exhibit stunning variation in size, structure, and function, which has allowed these animals to use their vision to adapt to a huge range of different environments and lifestyles, and evolve complex behaviors. Much of our knowledge of eye development has been learned from Drosophila, while visual adaptations and behaviors are often more striking and better understood from studies of other insects. However, recent studies in Drosophila and other insects, including bees, beetles, and butterflies, have begun to address this gap by revealing the genetic and developmental bases of differences in eye morphology and key new aspects of compound eye structure and function. Furthermore, technical advances have facilitated the generation of high‐resolution connectomic data from different insect species that enhances our understanding of visual information processing, and the impact of changes in these processes on the evolution of vision and behavior. Here, we review these recent breakthroughs and propose that future integrated research from the development to function of visual systems within and among insect species represents a great opportunity to understand the remarkable diversification of insect eyes and vision.

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“…Spatial and temporal morphogen gradients, as well as sequential expression of transcription factors, have been implicated in generating spinal neuron subtype diversity across species 77,78 . Birthdate-dependent subtype differences have been linked to temporally regulated post-mitotic transcription factor programs in vertebrates 79,80 and invertebrates 81,82 . We propose that Prdm16 and Evi1 operate as intrinsic determinants acting within a temporal program that defines MN functional subtype fates.…”

Section: Discussionmentioning

confidence: 99%

Determinants of Motor Neuron Functional Subtypes Important for Locomotor Speed

D’Elia

Hameedy

Goldblatt

et al. 2022

Preprint

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Locomotion requires precise control of the strength and speed of muscle contraction and is achieved by recruiting functionally-distinct subtypes of motor neurons (MNs). MNs are essential to movement and differentially susceptible in disease, but little is known about how MNs acquire functional subtype-specific molecular features during development. Using single cell RNA profiling in larval zebrafish, we identify novel and conserved molecular signatures for MN functional subtypes, and identify genes expressed in both early post-mitotic and mature MNs. Assessing MN development in genetic mutants, we define a molecular program essential for MN functional subtype specification. Two evolutionarily-conserved transcription factors, Prdm16 and Evi1, are both functional subtype-specific determinants integral for fast MN development. Loss ofprdm16orevi1causes fast MNs to develop transcriptional profiles and innervation similar to slow MNs. These results reveal the molecular diversity of vertebrate spinal MNs and demonstrate that functional subtypes are specified through intrinsic transcriptional codes.

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Coordinated control of neuronal differentiation and wiring by sustained transcription factors

Cited by 20 publications

References 67 publications

Morphological and functional convergence of visual projections neurons from diverse neurogenic origins inDrosophila

Morphological and functional convergence of visual projections neurons from diverse neurogenic origins inDrosophila

Looking across the gap: Understanding the evolution of eyes and vision among insects

Determinants of Motor Neuron Functional Subtypes Important for Locomotor Speed

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