Cooperative Adsorption of Proteins onto Hydroxyapatite

Luo, Qilie; Andrade, Joseph D.

doi:10.1006/jcis.1997.5364

Cited by 128 publications

(102 citation statements)

References 50 publications

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“…It is known that calcium phosphates, eg, HA, exhibit a high affinity for protein adsorption. 56 bone-specific proteins, such as BMP-2, osteocalcin, osteopontin, etc, were adsorbed by the bone-grafting substitute, which is structurally identical to the described coating material. Furthermore, it is reported that a proteinaceous matrix was formed.…”

Section: Discussionmentioning

confidence: 99%

In vivo and in vitro investigations of a nanostructured coating material – a preclinical study

Adam¹,

Ganz²,

Xu³

et al. 2014

IJN

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Immediate loading of dental implants is only possible if a firm bone-implant anchorage at early stages is developed. This implies early and high bone apposition onto the implant surface. A nanostructured coating material based on an osseoinductive bone grafting is investigated in relation to the osseointegration at early stages. The goal is to transmit the structure (silica matrix with embedded hydroxyapatite) and the properties of the bone grafting into a coating material. The bone grafting substitute offers an osseoinductive potential caused by an exchange of the silica matrix in vivo accompanied by vascularization. X-ray diffraction and transmission electron microscopy analysis show that the coating material consists of a high porous silica matrix with embedded nanocrystalline hydroxyapatite with the same morphology as human hydroxyapatite. An in vitro investigation shows the early interaction between coating and human blood. Energy-dispersive X-ray analysis showed that the silica matrix was replaced by an organic matrix within a few minutes. Uncoated and coated titanium implants were inserted into the femora of New Zealand White rabbits. The bone-to-implant contact (BIC) was measured after 2, 4, and 6 weeks. The BIC of the coated implants was increased significantly at 2 and 4 weeks. After 6 weeks, the BIC was decreased to the level of the control group. A histological analysis revealed high bone apposition on the coated implant surface after 2 and 4 weeks. Osteoblastic and osteoclastic activities on the coating material indicated that the coating participates in the bone-remodeling process. The nanostructure of the coating material led to an exchange of the silica matrix by an autologous, organic matrix without delamination of the coating. This is the key issue in understanding initial bone formation on a coated surface.

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Section: Discussionmentioning

confidence: 99%

In vivo and in vitro investigations of a nanostructured coating material – a preclinical study

Adam¹,

Ganz²,

Xu³

et al. 2014

IJN

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“…Preliminary experiments have indicated that the HA beads used in our laboratory are being coated with these dairy proteins, which is possibly contributing to a reduction in adherence of S. mutans. Adsorption of proteins to HA is quite complex since HA carries both positive and negative charges due to the presence of Ca 2+ and PO 4 3-, and the affinity of the former for negatively charged groups is higher than the affinity for phosphate groups for positively charged residues [29]. However, we cannot report that the inhibition of S. mutans to HA is solely due to this process, as there are other possible mechanisms that could be contributing to this inhibition, such as charge-chargerepulsion/attraction, hydrophobic interactions, steric hindrance and possible interference to the surface binding proteins associated with S. mutans.…”

Section: Assessment Of Anti-adhesive Effect Of Milk Componentsmentioning

confidence: 99%

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Halpin

O'Connor

McMahon

et al. 2008

Eur Food Res Technol

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The aim of this study was to investigate the inhibitory effect of various dairy powders and milk constituents on the adhesion of a clinical isolate of Streptococcus mutans to hydroxylapatite (HA), an analogue of tooth enamel. Adhesion of a microorganism to a cell surface such as epithelial cells or tooth enamel is considered to be the first step in pathogenesis. Inhibiting this process may have therapeutic effects in vivo. The adherence assays were performed by incubating S. mutans with HA in the presence of each test material for 45 min, followed by centrifugal separation of the HA. Unbound bacteria were then quantified using a fluorescent dye. Sweet and Acid WPC80, buttermilk powder and cream powder were found to very effectively inhibit adherence of S. mutans to phosphate-buffered saline coated HA (PBS-HA). Sodium caseinate and the casein fractions a-,b-and j-casein were also found to show high levels of anti-adhesive activity. A selection of test materials were assessed using saliva-coated HA (S-HA), and similar trends were observed. The results suggest commercial dairy powders, and certain milk proteins, can inhibit adhesion of S. mutans to HA and may have potential to control dental caries.

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“…(Figure 18) by using size-selected Au n (SG) m as a precursor. 171,172 HAP was selected as a support for AuCLs 173175 since it was expected to adsorb Au n (SG) m as efficiently as other biomolecules via hydrogen bonding and/or the electrostatic interaction 176 and that HAP could stabilize bare Au n clusters against sintering due to its strong interaction with the PO 4 3¹ moiety. 177 First, Au n (SG) m clusters with (n, m) = (10, 10), (18,14), (25,18), and (39,24) were prepared by the method described in Section 2.1 and were adsorbed on HAP (surface area: µ100 m 2 g…”

Section: Catalysis Formentioning

confidence: 99%

Toward an Atomic-Level Understanding of Size-Specific Properties of Protected and Stabilized Gold Clusters

Tsukuda

2012

Bulletin of the Chemical Society of Japan

228

230

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Metal clusters consisting of fewer than 100 atoms (diameter <2 nm) are highly promising as a new class of building units for functional materials because of their novel and size-dependent properties. Nevertheless, basic and applied studies of metals clusters have been hampered by the lack of specific guidelines for design and precise synthetic methods. This account surveys recent investigations of gold clusters focusing on our effort toward an atomic-level understanding and control of their size-specific properties. We have developed a size-controlled method for synthesizing gold clusters protected by ligands, stabilized by polymers, and supported on solids. Remarkable size-effects on stabilities and various properties including catalysis were observed. Their mechanisms are discussed based on fundamental knowledge of bare gold clusters in the gas phase.

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Cooperative Adsorption of Proteins onto Hydroxyapatite

Cited by 128 publications

References 50 publications

In vivo and in vitro investigations of a nanostructured coating material – a preclinical study

In vivo and in vitro investigations of a nanostructured coating material – a preclinical study

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Toward an Atomic-Level Understanding of Size-Specific Properties of Protected and Stabilized Gold Clusters

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Cooperative Adsorption of Proteins onto Hydroxyapatite

Cited by 128 publications

References 50 publications

In vivo and in vitro investigations of a nanostructured coating material &ndash; a preclinical study

In vivo and in vitro investigations of a nanostructured coating material &ndash; a preclinical study

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Toward an Atomic-Level Understanding of Size-Specific Properties of Protected and Stabilized Gold Clusters

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In vivo and in vitro investigations of a nanostructured coating material – a preclinical study

In vivo and in vitro investigations of a nanostructured coating material – a preclinical study