Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis

Zhu, Yihua; Ye, Yanfang; Wu, Zhengrong; Wu, Jian-Qiu

doi:10.1091/mbc.e13-06-0301

Cited by 39 publications

(97 citation statements)

References 120 publications

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“…Medial cortical nodes have been shown to contain six additional components so far, the two effectors that regulate mitotic entry, Cdr1 and Wee1, as well as a series of four non-essential components of the contractile ring, Blt1, Gef2, Klp8 and Nod1 (Jourdain et al, 2013;Moseley et al, 2009;Zhu et al, 2013). These four components remain associated with the contractile ring throughout its assembly and constriction phases and have been shown to regulate its function ( Goss et al, 2014;Guzman-Vendrell et al, 2013;Jourdain et al, 2013;Ye et al, 2012;Zhu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…These four components remain associated with the contractile ring throughout its assembly and constriction phases and have been shown to regulate its function ( Goss et al, 2014;Guzman-Vendrell et al, 2013;Jourdain et al, 2013;Ye et al, 2012;Zhu et al, 2013). Blt1 contains a membrane-binding motif in its C-terminus and can associate with the cell cortex independently of Cdr2 (GuzmanVendrell et al, 2013;Moseley et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…A second function of Cdr2 nodes is to recruit the anillin Mid1 (Almonacid et al, 2009;Chang et al, 1996;Moseley et al, 2009;Sohrmann et al, 1996) as well as a series of non-essential contractile ring components, such as Blt1, Gef2, Klp8 and Nod1 (Jourdain et al, 2013;Moseley et al, 2009;Zhu et al, 2013), to form precursors for the contractile ring and pre-establish the division plane on the medial cortex in parallel to Mid1 export from the nucleus (Almonacid et al, 2009;Bahler et al, 1998a;Paoletti and Chang, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Cdr2 is therefore considered to be the key organizer of medial cortical nodes. Moreover, besides cytokinetic functions, these components also participate in the mitotic-promoting function of Cdr2 with Wee1 and Cdr1 because their deletion delays mitotic entry (Jourdain et al, 2013;Moseley et al, 2009;Zhu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular control of the Wee1 regulatory pathway by the SAD kinase Cdr2

Guzmán-Vendrell

Rincón

Dingli

et al. 2015

Journal of Cell Science

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Cell growth and division are tightly coordinated to maintain cell size constant during successive cell cycles. In Schizosaccharomyces pombe, the SAD kinase Cdr2 regulates the cell size at division and the positioning of the division plane. Cdr2 forms nodes on the medial cortex containing factors that constitute an inhibitory pathway for Wee1. This pathway is regulated by polar gradients of the DYRK kinase Pom1, and involves a direct inhibitor of Wee1, the SAD kinase Cdr1. Cdr2 also interacts with the anillin Mid1, which defines the division plane, and with additional components of the medial cortical nodes, including Blt1, which participate in the mitotic-promoting and cytokinetic functions of nodes. Here, we show that the interaction of Cdr2 with Wee1 and Mid1 requires the UBA domain of Cdr2, which is necessary for its kinase activity. In contrast, Cdr1 associates with the C-terminus of Cdr2, which is composed of basic and KA-1 lipid-binding domains. Mid1 also interacts with the C-terminus of Cdr2 and might bridge the N-and C-terminal domains, whereas Blt1 associates with the central spacer region. We propose that the association of Cdr2 effectors with different domains might constrain Cdr1 and Wee1 spatially to promote Wee1 inhibition upon Cdr2 kinase activation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular control of the Wee1 regulatory pathway by the SAD kinase Cdr2

Guzmán-Vendrell

Rincón

Dingli

et al. 2015

Journal of Cell Science

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“…The anillin Mid1p moves from the nucleus and joins type 1 nodes during G2 (Morrell et al, 2004;Moseley et al, 2009;Paoletti and Chang, 2000;Wu et al, 2006). Type 2 nodes, composed of contractile ring proteins Blt1p, Klp8p, Nod1p and Gef2p (Jourdain et al, 2013;Moseley et al, 2009;Zhu et al, 2013), emerge from the contractile ring as it disassembles at the end of cytokinesis. During interphase, type 2 nodes diffuse in the cortex from the previous division site until they bind stationary type 1 nodes around the cell equator to form cytokinetic nodes (Akamatsu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The septation initiation network controls the assembly of nodes containing Cdr2p for cytokinesis in fission yeast

Akamatsu

Pollard

2014

Journal of Cell Science

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In the fission yeast Schizosaccharomyces pombe, cortical protein structures called interphase nodes help to prepare the cell for cytokinesis by positioning precursors of the cytokinetic contractile ring, and the septation initiation network (SIN) regulates the onset of cytokinesis and septum formation. Previous work has noted that one type of interphase node disappears during mitosis providing SIN activity is high. Here, we used time-lapse fluorescence microscopy to provide evidence that SIN activity is necessary and sufficient to disperse the type 1 node proteins Cdr2p and Mid1p into the cytoplasm, so these nodes assemble only during interphase through early mitosis when SIN activity is low. Activating the SIN in interphase cells dispersed Cdr2p and anillin Mid1p from type 1 nodes a few min after the SIN kinase Cdc7p-GFP accumulated at spindle pole bodies. If the SIN was then turned off in interphase cells, Cdr2p and Mid1p reappeared in nodes in parallel with the decline in SIN activity. Hyperactivating SIN during mitosis dispersed type 1 nodes earlier than normal, and prolonged SIN activation prevented nodes from reforming at the end of mitosis.

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High‐Performance Gel Polymer Electrolyte Based on Chitosan–Lignocellulose for Lithium‐Ion Batteries

et al. 2020

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A new gel polymer electrolyte (GPE) based on chitosan‐lignocellulose composites with a smooth and porous structure for applications in lithium‐ion batteries was prepared. The obtained GPE matrix and GPE show excellent comprehensive performances. The GPE matrix based on the composite containing 15 % chitosan (denoted CSLC‐15) had the best performance, with liquid electrolyte uptake of up to 749.1 wt %. The ionic conductivity for corresponding GPE is 2.89 mS cm−1 at room temperature, and the lithium ion transference number is 0.90. The GPE proved promising for use in lithium‐ion batteries, offering a large electrochemical window (4.8 V) and excellent interfacial compatibility (a stable impedance value of 227.97 Ω after 30 days). Moreover, the GPE shows excellent thermal and mechanical properties, and high C‐rates capability (163.13 mAh g−1, 152.53 mAh g−1, 144.27 mAh g−1, 128.45 mAh g−1, 156.12 mAh g−1, at 0.2 C, 0.5 C, 1.0 C, 2.0 C, 0.2 C, respectively) and cycle performance (161.99 mAh g−1 at the 99th cycle of 0.2 C), which proved its suitability of use in lithium‐ion batteries.

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Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis

Cited by 39 publications

References 120 publications

Molecular control of the Wee1 regulatory pathway by the SAD kinase Cdr2

Molecular control of the Wee1 regulatory pathway by the SAD kinase Cdr2

The septation initiation network controls the assembly of nodes containing Cdr2p for cytokinesis in fission yeast

High‐Performance Gel Polymer Electrolyte Based on Chitosan–Lignocellulose for Lithium‐Ion Batteries

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