Conversion of nonimmunogenic low molecular weight ragweed components to immunogens for induction of homocytotropic antibody

Attallah, N.A.; Kuroume, Takayoshi; Sehon, A.H.

doi:10.1016/0019-2791(75)90084-1

Cited by 8 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glutaraldehyde has previously been used to generate heterogeneous allergen mixtures which were examined in animal and clinical studies (17)(18)(19)(20)(21) . Although promising in terms of enhanced safety, these preparations failed to demonstrate marked decreases in IgE production and their mechanism of action remains unknown .…”

Section: Discussionmentioning

confidence: 99%

Anti-interferon gamma treatment blocks the ability of glutaraldehyde-polymerized allergens to inhibit specific IgE responses.

HayGlass

Stefura

1991

The Journal of Experimental Medicine

View full text Add to dashboard Cite

SummaryThe lymphokines interleukin 4 and interferon y (IFN-y) have been shown to play an important role in regulation of polyclonal immunoglobulin E (IgE) and IgG2a responses in vitro and in vivo . We demonstrate here that treatment with chemically modified ovalbumin (OA) results in long-lived, 97-99% inhibition of allergen-specific murine IgE responses and 10 3 -10 4 -fold increases in anti-OA IgG2a. Responses to unrelated antigens are not affected. Treatment with unmodified OA under the same conditions fails to inhibit primary or secondary IgE responses or to increase IgG2a but does lead to pronounced increases in OA specific IgG1 production . Glutaraldehyde-polymerized ovalbumin (OA-POL)-induced changes in IgE and IgG2a responses are abrogated by in vivo treatment with purified monoclonal anti-IFN-y antibody (XMG 1 .2), a finding indicative of preferential IFN-y production upon exposure to chemically modified, but not native, allergen. The results suggest the possibility that the pattern of cytokine synthesis elicited after exposure to protein antigens, and the resulting immune response, may be dependent upon the form of antigen to which the individual is exposed and consequently may be subject to manipulation .

show abstract

Section: Discussionmentioning

confidence: 99%

Anti-interferon gamma treatment blocks the ability of glutaraldehyde-polymerized allergens to inhibit specific IgE responses.

HayGlass

Stefura

1991

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…After dialysis and gel filtration (Biogel A-50m; Bio-Rad Laboratories, Mississauga, Ontario, Canada; Vo = 5 x 107), OA-POL was recovered as a single, sharp, symmetric peak with an average relative molecular weight of 3.5 x 107. This method was developed in preference to previous approaches to glutaraldehyde modification which have been found to yield highly heterogeneous mixtures of reaction products exhibiting diverse immunological effects (11)(12)(13). OVA or OA-POL treatment consisted of three 80/~g injections (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…Depending on the reaction conditions selected, products with a wide range of immunological characteristics can be obtained after chemical modification of protein antigens with glutaraldehyde (10)(11)(12)(13). Glutaraldehyde polymerized OVA (OA-POL), soluble OVA polymers of average relative molecular weight of 3.5 x 107, inhibits induction of IgE responses (14) and abrogates ongoing IgE synthesis (15) in a murine model of human immediate hypersensitivity.…”

mentioning

confidence: 99%

Chemically modified antigen preferentially elicits induction of Th1-like cytokine synthesis patterns in vivo.

Yang

Gieni

Mosmann

et al. 1993

The Journal of Experimental Medicine

View full text Add to dashboard Cite

SummaryDifferential activation of CD4 + T cell subsets in vivo leads to the development of qualitatively different effector responses. We identify an approach that allows selective activation of strongly Thl-dominated immune responses to protein antigens. Whereas in vivo administration of ovalbumin (OVA) induces cytokine synthesis that is neither Thl nor Th2 dominated, administration of glutaraldehyde polymerized, high relative molecular weight OVA (OA-POL) leads to 20-fold increase in the ratio of interferon 3' (IFN-3")/IL-4 and IFN-3"/IL-10 synthesis observed after shortterm, antigen-mediated restimulation directly ex vivo. In contrast, concurrent in vivo administration of anti-IFN-3/mAb and OVA or OA-POL results in marked increases in IL-4 and IL-10, and decreased IFN-3' production, reflecting a polarization of the response towards a Th2-1ike pattern of cytokine synthesis. These observations may be useful in clinical settings including hypersensitivity, autoimmune diseases, and vaccine development where the ability to actively select specific patterns of cytokine gene expression would be advantageous.

show abstract

“…Immunization of rabbits with the low molecular weight po lymers resulted in increased production of IgG antibodies while the high molecular weight polymers were less immunogenic even than soluble antigen E. The IgE anti body response to these preparations also de pended on the molecular size; however, the highest response was obtained in rabbits im munized with the high molecular weight po-Iymer (4-206 daltons). In a more recent re port, a nonimmunogenic, dialyzable, small molecular weight constituent of a water-sol uble extract of ragweed pollen, was convert ed into a relatively good immunogen for IgE antibody production in rats [2]. In contrast with these observations, Wheeler et al [39] have shown that rats immunized with glutaraldehyde-polymerized crude timothy pol len extract and B. pertussis produced mark edly decreased IgE antibody levels and that the decrease was proportional to the degree of antigen polymerization.…”

Section: Discussionmentioning

confidence: 99%

Reaginic Antibody Production to <i>Ascaris suum</i> Allergen, ASC-1

Strejan¹,

Surlan²

1977

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Rats immunized with a purified Ascaris suum allergen (Asc-1) or with its dinitrophenylated derivate (DNP-Asc-1) produced high levels of reaginic (IgE) antibodies. A second injection of antigen given 30 days later did not result in an anamnestic IgE antibody response. Immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (ATxB) rats with soluble Asc-1 or DNP-Asc-1 failed to stimulate reaginic antibody production. The administration of glutaraldehyde-polymerized antigen induced in some but not all ATxB rats, low but detectable levels of IgE antibodies. These levels increased following a second injection of nonpolymerized antigen in Al(OH)₃ gels. Priming of animals with poylmerized carrier and Bordetella pertussis did not stimulate a primary anticarrier IgE response but led to an enhanced antihapten IgE response following the administration of soluble DNP-Asc-1 in Al(OH)₃. The results are consistent with the notion that a sharply reduced but clearly functional T-derived helper cell population could be triggered by the polymerized but not by the soluble form of the immunogen.

show abstract

Conversion of nonimmunogenic low molecular weight ragweed components to immunogens for induction of homocytotropic antibody

Cited by 8 publications

References 7 publications

Anti-interferon gamma treatment blocks the ability of glutaraldehyde-polymerized allergens to inhibit specific IgE responses.

Anti-interferon gamma treatment blocks the ability of glutaraldehyde-polymerized allergens to inhibit specific IgE responses.

Chemically modified antigen preferentially elicits induction of Th1-like cytokine synthesis patterns in vivo.

Reaginic Antibody Production to <i>Ascaris suum</i> Allergen, ASC-1

Contact Info

Product

Resources

About