Conversion of Cellulosic waste into fermentable sugar: Process optimization

Salam, Muhammad Abdus; Pondith, Paritush Chandra; Islam, Ariful; Khan, Maksudur R.; Uddin, Mohammed Rakib; Islam, M. A.

doi:10.3329/jce.v28i1.18107

Cited by 13 publications

(6 citation statements)

References 12 publications

(8 reference statements)

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“…It worth mentioning that the highest ever measured glucose concentration was recorded as 5.3 mg/ml when the rice waste was treated with 20% H 3 PO 4 followed by autoclaving. Our data are in agreement with [28] and [29] who mentioned that the diluted acids are preferred for the liberation of glucose units from wastes. However, the applied autoclaving technique would improve the pre-hydrolysis process as it can apply the combination of pressure and high temperature and hence is considered a potent physical treatment method [30].…”

Section: Combined Chemical/physical Pre-hydrolysis Of Rice Wastesupporting

confidence: 93%

Preparation and characterization of Polyvinyl chloride/Polymethyl- methacrylate/Graphite [PVC/PMMA/G] membrane used for the purification of the bioethanol produced from the hydrolysis and fermentation of rice waste

Taha

Taha²,

Saied³

et al. 2023

Egypt. J. Chem.

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Rice waste as a starchy food waste that is discharged daily into the environment in huge amounts has been used as a valuable source for the production of bioethanol. The tested parameters investigated that phosphoric acid as a chemical representative combined with autoclaving as a physical representative are the most potent conditions for the liberation of glucose units from the starch molecules among other tested conditions. The optimization process revealed that the maximum production of simple sugars of 12.39 mg/ml from rice waste can be obtained through the pre-treatment of 5% rice waste with 20% H3PO4 and autoclaving followed by the amylase hydrolysis. These liberated glucose units have been successfully fermented into 5.2 mg/ml bioethanol using a yeast strain (Pichia nakasei with the accession number OK092294). The separation of the produced bioethanol from other media components was performed via amicon cell ultrafiltration system integrated with polymeric membrane. The membrane was prepared from Polyvinyl chloride/Polymethylmethacrylate/ Graphite [PVC/PMMA/G] with different thicknesses 400, 500 and 600 µm. For membrane characterization, Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile strength, water contact angle, and water and ethanol uptakes have been used. Among the tested membranes thicknesses, the obtained results showed that the best membrane thickness is 500 µm which recoded 939.526 (mg/m2.h) and 10.557 for flux of permeate and separation factor, respectively.

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Section: Combined Chemical/physical Pre-hydrolysis Of Rice Wastesupporting

confidence: 93%

Preparation and characterization of Polyvinyl chloride/Polymethyl- methacrylate/Graphite [PVC/PMMA/G] membrane used for the purification of the bioethanol produced from the hydrolysis and fermentation of rice waste

Taha

Taha²,

Saied³

et al. 2023

Egypt. J. Chem.

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“…Based on the above findings, it can be concluded that the optimum growth conditions for most of the isolated bacteria were: the temperature of 30°C and pH 7, what is very important in the context of hydrolysis of cellulose. Although there have been diverse reports on the optimal initial pH and temperature for cellulolytic enzymes production, it is assumed that most efficient enzymatic hydrolysis occurs at 30°C and pH 7 ( Pradip Saha et al, 2012 ; Salam et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production

et al. 2016

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The use of lignocellulosic biomass as a substrate in agricultural biogas plants is very popular and yields good results. However, the efficiency of anaerobic digestion, and thus biogas production, is not always satisfactory due to the slow or incomplete degradation (hydrolysis) of plant matter. To enhance the solubilization of the lignocellulosic biomass various physical, chemical and biological pretreatment methods are used. The aim of this study was to select and characterize cellulose-degrading bacteria, and to construct a microbial consortium, dedicated for degradation of maize silage and enhancing biogas production from this substrate. Over 100 strains of cellulose-degrading bacteria were isolated from: sewage sludge, hydrolyzer from an agricultural biogas plant, cattle slurry and manure. After physiological characterization of the isolates, 16 strains (representatives of Bacillus, Providencia, and Ochrobactrum genera) were chosen for the construction of a Microbial Consortium with High Cellulolytic Activity, called MCHCA. The selected strains had a high endoglucanase activity (exceeding 0.21 IU/mL CMCase activity) and a wide range of tolerance to various physical and chemical conditions. Lab-scale simulation of biogas production using the selected strains for degradation of maize silage was carried out in a two-bioreactor system, similar to those used in agricultural biogas plants. The obtained results showed that the constructed MCHCA consortium is capable of efficient hydrolysis of maize silage, and increases biogas production by even 38%, depending on the inoculum used for methane fermentation. The results in this work indicate that the mesophilic MCHCA has a great potential for application on industrial scale in agricultural biogas plants.

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“…After dilute acid pretreatment, enzymatic hydrolysis at the solid loading of 5% dry mass (DM, w/w) of each sample was performed using cellulase and β-glucosidase for a maximum of 72 h in a water bath at 37°C separately. Sodium citrate buffer (50 mM, pH 4.8) is used in the mixture to maintain the pH at 4.8 [23].…”

Section: Enzymatic Saccharification and Hydrolysismentioning

confidence: 99%

Paper Waste Management: Extraction of Fermentable Sugar from Lignocellulosic Waste Paper

Hayat¹,

Rafiq

Anwar³

2021

Pak J Anal Environ Chem

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The utilization of paper on a commercial scale is increasing day by day throughout the world that produces million of tons of paper waste yearly and burdened for landfills. The present study focuses on the exploitation of waste papers (office paper, newspaper and tissue paper) as a cheapest alternative source of energy to extract fermentable sugar by applying chemical and enzymatic pretreatments. The quantification of released sugar was analyzed by spectrophotometer and high performance liquid chromatography refractive index (HPLC-RI) detector. Cellulose (12 FPU/g) and β-glucosidase (12 FPU/g) was found to be effective for the extraction of fermentable sugar from paper waste. The contents of cellulose (C6H10O5)n, hemicellulose (C5H10O5)n and lignin (C9H10O2,C10H12O3,C11H14O4) found in office paper were 40%, 32.5%, 22.5%, in newspaper 46.5%, 30.5% 22.5%, and in tissue paper 62%, 22%, 15.5%, respectively. The percentages of sugar contents assessed in this study were 62% in tissue paper and 46.5% in newspaper and 40% in office papers. Among the three substrates, tissue paper (23.4 mg/mL) released a significant amount of glucose (C6H6O12), whereas newspaper (20.8 mg/mL) and office paper (19.6 mg/mL) released less amount of sugar. This research of acid pre-treatment and enzymatic hydrolysis was an efficient method to improve glucose conversion from lignocellulosic waste. Furthermore, this approach can be proved the first step towards the sustainable production of bioethanol from wastepaper-extracted sugar.

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Conversion of Cellulosic waste into fermentable sugar: Process optimization

Cited by 13 publications

References 12 publications

Preparation and characterization of Polyvinyl chloride/Polymethyl- methacrylate/Graphite [PVC/PMMA/G] membrane used for the purification of the bioethanol produced from the hydrolysis and fermentation of rice waste

Preparation and characterization of Polyvinyl chloride/Polymethyl- methacrylate/Graphite [PVC/PMMA/G] membrane used for the purification of the bioethanol produced from the hydrolysis and fermentation of rice waste

Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production

Paper Waste Management: Extraction of Fermentable Sugar from Lignocellulosic Waste Paper

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