Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production

Poszytek, Krzysztof; Ciezkowska, Martyna; Skłodowska, Aleksandra; Drewniak, Łukasz

doi:10.3389/fmicb.2016.00324

Cited by 102 publications

(37 citation statements)

References 43 publications

(29 reference statements)

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“…Daily bioaugmentation also proved more effective than a one-time application as a washout in a continuous reaction with short HRT quickly decreases the positive impact [63]. Similar results of 15% higher sCOD and an increase of 38% in biogas yield with bioaugmentation of a selected microbial consortia with high cellulolytic activity in a batch digestion of maize silage were published [64]. On the contrary, bioaugmentation with the fungus Piromyces rhizinflata to the hydrolysis stage did not improve digestion significantly [65].…”

Section: Combined Pretreatmentmentioning

confidence: 53%

Role of Microbial Hydrolysis in Anaerobic Digestion

2020

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There is a growing need of substrate flexibility for biobased production of energy and value-added products that allows the application of variable biodegradable residues within a circular economy. It can be used to balance fluctuating energy provision of other renewable sources. Hydrolysis presents one of the biggest limitations during anaerobic digestion. Methods to improve it will result in broader process applicability and improved integration into regional material cycles. Recently, one focus of anaerobic digestion research has been directed to systems with a separate hydrolysis–acidogenesis stage as it might be promised to improve process performance. Conditions can be adjusted to each class of microorganisms individually without harming methanogenic microorganisms. Extensive research of separate biomass pretreatment via biological, chemical, physical or mixed methods has been conducted. Nevertheless, several methods lack economic efficiency, have a high environmental impact or focus on specific substrates. Pretreatment via a separate hydrolysis stage as cell-driven biotransformation in a suspension might be an alternative that enables high yields, flexible feeding and production, and a better process control. In this review, we summarize existing technologies for microbial hydrolytic biotransformation in a separate reactor stage and the impacts of substrate, operational parameters, combined methods and process design as well as remaining challenges.

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Section: Combined Pretreatmentmentioning

confidence: 53%

Role of Microbial Hydrolysis in Anaerobic Digestion

2020

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“…In order to increase the methanogenesis efficiency and the methane content in the produced biogas, researchers often introduce some additional cells into the methane tank besides the anaerobic sludge. These microorganisms usually stimulate the processes at various stages of the cellulose-containing substrates' conversion into methane: hydrolysis of complex substrates [1,2,37], additional formation of hydrogen [38] and methane [39].…”

Section: Introductionmentioning

confidence: 99%

Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

et al. 2019

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The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional” introduction of bacterial Clostridium acetobutylicum, Pseudomonas sp., Enterococcus faecalis cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge.

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“…and Fibrobacteraceae sp, the efficiency of biogas production from rice straw in anaerobic digester was increased by 9.3% when using the optimized operational condition [8]. Similarly, using a mesophilic microbial consortium consisting of 3 genera of bacteria, including Bacillus, Providencia, and Ochrobactrum, to decompose maize silage at 30°C and 37°C, it was found that the biogas production was increased by 38% [9]. Subcultivation and enrichment of microbial consortium obtained from swine manure were demonstrated to improve the biogas yield up to 40% produced from anaerobic digestion of lignocellulose [10].…”

Section: Introductionmentioning

confidence: 93%

Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

et al. 2020

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Biogas production is one of the means to manage the cassava pulp waste obtained from the cassava processing plants. The success of the process is determined by the hydrolysis in an anaerobic digester. When the digester failure is found, the new microbial consortium inoculum is introduced to the system with the long period of set up time. This research aimed to construct the endemic microbial consortium by re-cultivating the cellulolytic microbial consortia obtained from cassava pulp and digester wastewater with the expected shorter set up time. Modifications of enrichment and re-cultivation methods by varying the nutrients, pH and temperature improved the enzymatic hydrolysis yields, as reducing sugars, of CMC, rice straw and cassava pulp substrates approximately 9, 3, and 13 times, respectively. To analyze the enzymatic activities of the selected microbial consortia, the cellulase enzyme was extracted, partially purified and analyzed on CMC-zymogram. The ~130 kDa-sized cellulase enzyme was identified with endocellulase activity, and it was considered as a relatively large molecular size molecule compared to most bacterial endocellulases. The selected microbial consortia were tested for their biomass degradation capacities, and the optimal operational condition was obtained at pH 7.0 and 30 °C. This optimal condition showed the proof of the concept that this re-cultivated consortium could be applied in on-site digester with high efficiency.

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Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production

Cited by 102 publications

References 43 publications

Role of Microbial Hydrolysis in Anaerobic Digestion

Role of Microbial Hydrolysis in Anaerobic Digestion

Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

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