1974
DOI: 10.1016/0006-8993(74)90330-8
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Conversion of [4,5-3H] leucine into 3H2O and tritiated metabolites in rat brain tissue. Comparison of a peripheral and intracranial route of administration

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Cited by 25 publications
(12 citation statements)
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“…The in uiuo incorporation rates of leucine into proteins of the spinal cord, of brain stem and of heart muscle were measured using a pulse of tritiated leucine by intraperitoneal injection after which a rather uniform labelling would be expected throughout the organism (Figure 1, Table 1) (Rees, Brogan, Entingh, Dunn, Shinkman, Damstra-Entingh, Wilson & Glassman, 1974). A relatively short incorporation period (20 min) was chosen for two reasons: (a) uptake and metabolism of the radioactive precursor in the tissue was fast (Schotman, Gispen, Jansz & De Wied, 1972;Schotman, Gipon & Gispen, 1974) and (b) incorporation was taken as measure of synthesis rather than turn-over rate of proteins. The incorporation into proteins was expressed as per cent of total radioactivity.…”
Section: Discussionmentioning
confidence: 99%
“…The in uiuo incorporation rates of leucine into proteins of the spinal cord, of brain stem and of heart muscle were measured using a pulse of tritiated leucine by intraperitoneal injection after which a rather uniform labelling would be expected throughout the organism (Figure 1, Table 1) (Rees, Brogan, Entingh, Dunn, Shinkman, Damstra-Entingh, Wilson & Glassman, 1974). A relatively short incorporation period (20 min) was chosen for two reasons: (a) uptake and metabolism of the radioactive precursor in the tissue was fast (Schotman, Gispen, Jansz & De Wied, 1972;Schotman, Gipon & Gispen, 1974) and (b) incorporation was taken as measure of synthesis rather than turn-over rate of proteins. The incorporation into proteins was expressed as per cent of total radioactivity.…”
Section: Discussionmentioning
confidence: 99%
“…The observed incorporation of radioactivity into TCA-insoluble proteins and the transfer of label to tritiated water are well-known phenomena in in vivo fate studies of labeled peptides and amino acids (Schotman et al, 1974;Verhoef and Witter, 1976;de Wildt et al, 1982). The metabolite profiles in rat plasma and brain tissue obtained after S.C. administration of [3H]PLG, with respect to both the presence of [3H]leucine as the major radioactively labeled metabolite and the finding of [3H]Leu-Gly-NH, as the sole labeled peptide intermediate in only trace amounts, are in accordance with those obtained after in vitro incubations of PLG with plasmaherurn (Walter et al, 1975;Witter et al, 1980) or brain subcellular preparations (Marks et al, 1974;Hui et al, 1980;de Wildt et al, 1982), as well as to those found after i.v.…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, metabolic inhibitors (anesthetics and sublethal amounts of cya nide) inhibited 3H20 formation, which is consistent with a depression of leucine me tabolism. Thus, the large proportion of la beled w'ater appearing in the adult rat imme diately following a pulse of tritiated leucine, administered subcutaneously [9,18], intrave nously [1], or intraperitoneally (as reported here), results from enzyme catalyzed reac tions.…”
Section: Discussionmentioning
confidence: 99%
“…Although leucine administered by an intraperitoneal route is rapidly degraded, any leucine transported into the brain [12,16] is primarily synthesized into protein [11,15,16,24]. Similarly, there is relatively little catabolism of leucine administered directly into the brain by injection [18].…”
Section: Discussionmentioning
confidence: 99%
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