Conversion and stability studies on enzyme-membrane reactor with lipase immobilized by different methods

Technical aspects of separation and simultaneous enzymatic reaction in multiphase enzyme membrane reactors

“…In multiphase enzyme membrane reactors the organic phase is often the substrate itself (e.g. oleic acid [32,50]). …”

“…In case of hydrolytic processes catalysed by lipase, the by-product alcohol is extracted by the aqueous phase through the membrane [45,47,53]. The esteri®cation in EMR is accompanied usually by extraction of alcohol to the organic phase and water produced to the aqueous phase [32,50]. The different cases of the realisation methods of the extraction process in enzyme membrane reactors are summarised in Table 6.…”

“…The formation of the covalent bonding is more complicated in membrane reactors than in the apparatuses with particulate solid support material. A simple but not selective chemical bond results by the spreading of a mixture of the concentrated enzyme solution and silicone on the membrane and by subsequent drying of the layer in nitrogen¯ow [50]. Special methods are which activate poly(ether)sulfone membrane blending it with polyacrolein as well as PVC sheets that embedded aldehydefunctionalised silica.…”

“…In the integrated system [50], a¯uidised-bed reactor with Ca-alginate entrapped Saccharomyces bayanus IST 154 cells was selected as a fermenter of high operational stability and volumetric productivity. No distributor plate for the¯uid¯ow was built into the reactor for the sake of simplicity.…”