Technical aspects of separation and simultaneous enzymatic reaction in multiphase enzyme membrane reactors

Sisak, Cs.; Nagy, Endre; Burfeind, Jens; Schügerl, K.

doi:10.1007/s004499900187

Cited by 13 publications

(4 citation statements)

References 35 publications

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“…Recently, LVVh‐7 extractions from complex hydrolysates have successfully been achieved by the use of solvents such as butan‐2‐ol [19] or a water/butan‐2‐ol/octan‐1‐ol mixture [20]. Furthermore, some workers have employed coupled solvent molecule extraction to membrane‐reactor production [21]. So we envisage that it may be possible to couple solvent extraction of LVVh‐7 with a continuous membrane reactor in order to get a continuous production of an LVVh‐7‐enriched peptide fraction.…”

Section: Resultsmentioning

confidence: 99%

Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor

Kapel¹,

Froidevaux²,

Nedjar‐Arroume³

et al. 2003

Biotech and App Biochem

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Peptic hydrolysis of native bovine haemoglobin at pH 3 yields the LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; LVVh-7) opioid peptide corresponding to the residues-31-40 fragment of the beta-chain of haemoglobin. This peptide is intermediate in the course of batch hydrolysis and is rapidly degraded. Indeed, it shows an optimum at 3% degree of hydrolysis (i.e. 2 min of reaction time). The hydrolysis was carried out in a continuous membrane reactor with a space time (ratio of the flux to the reactor volume) set to 2 min (corresponding to optimum LVVh-7 production). This process allows the continuous production of a constant fraction of intermediate peptides containing LVVh-7 for 48 min.

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Section: Resultsmentioning

confidence: 99%

Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor

Kapel¹,

Froidevaux²,

Nedjar‐Arroume³

et al. 2003

Biotech and App Biochem

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“…Some of these disadvantages can be overcome in an enzyme-based reactor, since enzymes are able to operate over a broad concentration range [16,17]. The potential advantages of this enzymatic treatment, as compared to *Corresponding author: Chinnathambi Velu, Research Associate, ARMATS BIOTEK Research Institute, Kottur, Chennai, India-600085, E-mail: chinna.velu1@ gmail.com conventional treatments, include application to recalcitrant materials, operation at high and low contaminant concentrations over a wide pH, temperature and salinity range.…”

Section: Introductionmentioning

confidence: 99%

Insilico Screening and Comparative Study on the Effectiveness of Textile Dye Decolourization by Crude Laccase Immobilised Alginate Encapsulated Beads from Pleurotus Ostreatus

Velu¹,

Veeramani²,

Suntharam³

et al. 2011

J Bioproces Biotechniq

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The necessity to safeguard the environment has increased the potential of enzyme usage in textile processing to ensure eco-friendly production. Laccase enzyme formulation has been used in textile processing such as biobleaching, dyeing, rove scouring, finishing, neps removal, printing, wash-off treatment, dye synthesis and effluent treatment. However, a high cost associated with biocatalyst production is still a hindrance to their use. Pleurotus ostreatus is a white-rot fungus that produces a ligninolytic enzyme complex rich in several laccase iso-enzymes. The main objective of this study is optimize influence of pH and stability of divalent metal ion-immobilzed crude laccase enzyme towards decolourization of prototype textile dyes such as Reactive red 80 (Red F3B) and Reactive blue 21(T Blue G). Wheat bran is used as a lead candidature for production of lignolytic enzyme using Pleurotus osteratus by solid state fermentation. Two divalent metal ions such as Zn2 + and Ca2 + were selected to study the influence of metal ions towards the dye decolourization. Results revealed that Ca2 + ion was better compared to Zn2 + ion towards enzyme immobilization and its influence on dye decolourization in the optimal pH 5.5. Finally, interactions between laccase and dyes were studied exclusively using Insilco structure based molecular docking methods.

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“…For these reasons, the integration of the chemical (biochemical) reaction with the classical extraction process is problematic and technologically inefficient. One method for the effective integration of the processes described above is the use of membrane contactors (Huang et al, 2004;Sisak et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of hydrophobic compounds integrated with membrane separation

Noworyta

Trusek-Hołownia

2016

Chemical and Process Engineering

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The enzymatic synthesis of a highly hydrophobic product (dipeptide precursor) in which the reaction is accompanied by the mass transfer of the reaction product to the organic phase and the substrates to the water phase is considered. Equations describing both continuous and batch processes are formulated. The range of variability in the operating parameters of such a bioreactor is specified, and the correlations reported in the literature to describe mass transfer in the membrane contactor are validated. The proposed process was verified experimentally, and good agreement between the determined and calculated concentrations was obtained in both phases.

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Technical aspects of separation and simultaneous enzymatic reaction in multiphase enzyme membrane reactors

Cited by 13 publications

References 35 publications

Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor

Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor

Insilico Screening and Comparative Study on the Effectiveness of Textile Dye Decolourization by Crude Laccase Immobilised Alginate Encapsulated Beads from Pleurotus Ostreatus

Enzymatic synthesis of hydrophobic compounds integrated with membrane separation

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