Converse Smith-Martin cell cycle kinetics by transformed B lymphocytes

Pham, Kim; Kan, Andrey; Whitehead, Lachlan; Hennessy, Robert J.; Rogers, Kelly L.; Hodgkin, Philip D.

doi:10.1080/15384101.2018.1511511

Cited by 4 publications

(7 citation statements)

References 51 publications

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“…Cell cycle period and phase durations of mammalian cells demonstrate a high degree of heterogeneity under culture conditions − and within a tumor microenvironment. , Such heterogeneities often significantly influence cell fate decision-making. − This means that these heterogeneities can be fine-tuned to design better therapeutics if the factors controlling these variabilities are known precisely. However, identifying these controlling factors is highly challenging due to the diverse and complex nature of cell cycle duration heterogeneities.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Fluctuations Govern the Serum-Dependent Cell Cycle Duration Heterogeneities in Mammalian Cells

et al. 2022

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Mammalian cells exhibit a high degree of intercellular variability in cell cycle period and phase durations. However, the factors orchestrating the cell cycle duration heterogeneities remain unclear. Herein, by combining cell cycle network-based mathematical models with live single-cell imaging studies under varied serum conditions, we demonstrate that fluctuating transcription rates of cell cycle regulatory genes across cell lineages and during cell cycle progression in mammalian cells majorly govern the robust correlation patterns of cell cycle period and phase durations among sister, cousin, and mother–daughter lineage pairs. However, for the overall cellular population, alteration in the serum level modulates the fluctuation and correlation patterns of cell cycle period and phase durations in a correlated manner. These heterogeneities at the population level can be fine-tuned under limited serum conditions by perturbing the cell cycle network using a p38-signaling inhibitor without affecting the robust lineage-level correlations. Overall, our approach identifies transcriptional fluctuations as the key controlling factor for the cell cycle duration heterogeneities and predicts ways to reduce cell-to-cell variabilities by perturbing the cell cycle network regulations.

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Section: Introductionmentioning

confidence: 99%

Transcriptional Fluctuations Govern the Serum-Dependent Cell Cycle Duration Heterogeneities in Mammalian Cells

et al. 2022

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“…Identifying the governing factors that organize the cell duration heterogeneities is a challenging task (8,9,12,17,(19)(20)(21)(22). In this article, we combined live-cell imaging studies of FUCCI-HeLa cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For example, The cell cycle phase durations quantified at the single-cell level in proliferating lymphocytes (8,17) using FUCCI-reporter (18) showed a highly variable S-G2-M time with strongly correlated G1 timings in the sibling cell pairs. A highly correlated cell cycle in sibling pairs has been observed in other mammalian cell types as well (19)(20)(21)(22) with mothers and daughters having poor correlation in cell cycle timings, while cousins showing a significant correlation for the same (19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional fluctuations govern the serum dependent cell cycle duration heterogeneities in Mammalian cells

Govindaraj

Sarma

Karulkar

et al. 2022

Preprint

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Mammalian cells exhibit a high degree of intercellular variability in cell cycle period and phase durations. However, the factors orchestrating the cell cycle duration heterogeneities remain unclear. Herein, by combining cell cycle network-based mathematical models with live single-cell imaging studies under varied serum conditions, we demonstrate that fluctuating transcription rates of cell cycle regulatory genes across cell lineages and during cell cycle progression in mammalian cells majorly govern the robust correlation patterns of cell cycle period and phase durations among sister, cousin, and mother-daughter lineage pairs. However, for the overall cellular population, alteration in serum level modulates the fluctuation and correlation patterns of cell cycle period and phase durations in a correlated manner. These heterogeneities at the population level can be fine-tuned under limited serum conditions by perturbing the cell cycle network using a p38-signaling inhibitor without affecting the robust lineage level correlations. Overall, our approach identifies transcriptional fluctuations as the key controlling factor for the cell cycle duration heterogeneities, and predicts ways to reduce cell-to-cell variabilities by perturbing the cell cycle network regulations.

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“…In addition, an abundance in Bacteroides and Prevotella was noted. This condition can lead to an imbalance of the three cross-talking axes, causing global imbalance of the body's homeostasis [124][125][126][127][128].…”

Section: Microbiota and H Pylori Interactionsmentioning

confidence: 99%

40 Years of Helicobacter pylori: A Revolution in Biomedical Thought

Charitos

D’Agostino

Topi

et al. 2021

Gastroenterology Insights

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Background: Various microorganisms such as bacteria, virus, and fungi can infect humans and cause not just a simple infection but septic conditions, organ dysfunction, and precancerous conditions or cancer involving various organ systems. After the discovery of the microscope, it was easier to discover and study such microorganisms, as in the case of Helicobacter pylori, a pathogen that was seen in the distant era of the nineteenth century but without being recognized as such. It took 100 years to later discover the pathogenesis and the cancer that this bacterium can cause. Since it was discovered, until today, there has been a continuous search for the understanding of its pathogenetic mechanisms, and the therapeutic approach is continuously updated. Methods: We investigated how diagnosis and therapy were dealt with in the past and how researchers sought to understand, exactly, the pathogenetic biomolecular mechanisms of H. pylori, from the genesis of the infection to the current knowledge, with an analysis of carcinogenic mechanisms in the stomach. We have examined the scientific evolution of the knowledge of the disease over these 40 years in the gastroenterological and pharmacological fields. This was possible through a search in the databases of Medline, the WHO website, the Centers for Disease Control and Prevention (CDC) website, PubMed, and Web of Science to analyze the earlier and the latest data regarding H. pylori. Results: With the scientific discoveries over time, thanks to an increasing number of progressions in scientific research in the analysis of the gastric mucosa, the role of Helicobacter pylori in peptic ulcer, carcinogenesis, and in some forms of gastric lymphoma was revealed. Furthermore, over the years, the biomolecular mechanism involvement in some diseases has also been noted (such as cardiovascular ones), which could affect patients positive for H. pylori. Conclusions: Thanks to scientific and technological advances, the role of the bacterium H. pylori in carcinogenesis has been discovered and demonstrated, and new prospective research is currently attempting to investigate the role of other factors in the stomach and other organs. Cancer from H. pylori infection had a high incidence rate compared to various types of cancer, but in recent years, it is improving thanks to the techniques developed in the detection of the bacterium and the evolution of therapies. Thus, although it has become an increasingly treatable disease, there is still continuous ongoing research in the field of treatment for resistance and pharma compliance. Furthermore, in this field, probiotic therapy is considered a valid adjuvant.

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Converse Smith-Martin cell cycle kinetics by transformed B lymphocytes

Cited by 4 publications

References 51 publications

Transcriptional Fluctuations Govern the Serum-Dependent Cell Cycle Duration Heterogeneities in Mammalian Cells

Transcriptional Fluctuations Govern the Serum-Dependent Cell Cycle Duration Heterogeneities in Mammalian Cells

Transcriptional fluctuations govern the serum dependent cell cycle duration heterogeneities in Mammalian cells

40 Years of Helicobacter pylori: A Revolution in Biomedical Thought

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