Convergence in probiotic <i>Lactobacillus</i> gut-adaptive responses in humans and mice

Marco, Maria L.; Vries, Maaike C. de; Wels, Michiel; Molenaar, Douwe; Mangell, Peter; Ahrné, Siv; Vos, Willem M. de; Vaughan, Elaine E.; Kleerebezem, Michiel

doi:10.1038/ismej.2010.61

Cited by 92 publications

(77 citation statements)

References 18 publications

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“…The possibility of evaluating more simplified, defined communities on the host metabolomic profile is a promising avenue to gain mechanistic insights. Simplified and defined model systems have been used extensively to study functional properties of common gut residents in vivo (Sonnenburg et al, 2006;Samuel et al, 2007;Marco et al, 2010;Rey et al, 2010;Faith et al, 2011). Our use of one-and two-member model communities indicates that there are aspects of the host metabolome that are conserved between these simplified ecosystems and an entire human microbiota, specifically the common depletion of metabolites that are present in GF mice.…”

Section: Discussionmentioning

confidence: 99%

A metabolomic view of how the human gut microbiota impacts the host metabolome using humanized and gnotobiotic mice

et al. 2013

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Defining the functional status of host-associated microbial ecosystems has proven challenging owing to the vast number of predicted genes within the microbiome and relatively poor understanding of community dynamics and community-host interaction. Metabolomic approaches, in which a large number of small molecule metabolites can be defined in a biological sample, offer a promising avenue to 'fingerprint' microbiota functional status. Here, we examined the effects of the human gut microbiota on the fecal and urinary metabolome of a humanized (HUM) mouse using an optimized ultra performance liquid chromatography-mass spectrometry-based method. Differences between HUM and conventional mouse urine and fecal metabolomic profiles support host-specific aspects of the microbiota's metabolomic contribution, consistent with distinct microbial compositions. Comparison of microbiota composition and metabolome of mice humanized with different human donors revealed that the vast majority of metabolomic features observed in donor samples are produced in the corresponding HUM mice, and individual-specific features suggest 'personalized' aspects of functionality can be reconstituted in mice. Feeding the mice a defined, custom diet resulted in modification of the metabolite signatures, illustrating that host diet provides an avenue for altering gut microbiota functionality, which in turn can be monitored via metabolomics. Using a defined model microbiota consisting of one or two species, we show that simplified communities can drive major changes in the host metabolomic profile. Our results demonstrate that metabolomics constitutes a powerful avenue for functional characterization of the intestinal microbiota and its interaction with the host.

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Section: Discussionmentioning

confidence: 99%

A metabolomic view of how the human gut microbiota impacts the host metabolome using humanized and gnotobiotic mice

et al. 2013

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“…It would be very useful if knowledge of strains, which could be defined as probiotics for one species, could also be used in other species. A recent study indicates that Lactobacillus plantarum 299v specifically is able to adapt its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and proteinaceous cell surface compounds -E-mail: paolo.bosi@unibo.it (Marco et al, 2010); this finding gives an important evidence about the dynamic metabolism of the bacteria, suggesting that the intestinal environment can modify the interplay among the bacteria and the host. There are evidences that in mammals, some gut microbes have diversified into hostadapted lineages, originated by a long-term evolutionary process, and that led to the development of a highly specialized symbiosis (Oh et al, 2010).…”

Section: Introductionmentioning

confidence: 95%

Dietary addition of Lactobacillus rhamnosus GG impairs the health of Escherichia coli F4-challenged piglets

Trevisi

Casini

Coloretti

et al. 2011

Animal

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Lactobacillus rhamnosus GG (LGG) is a probiotic for humans and is normally not found in pigs; however, it has been shown to protect the human-derived intestinal Caco-2 cells against the damage induced by an important intestinal pathogen, enterotoxigenic Escherichia coli F4 (ETEC). An experiment was conducted to test whether the dietary addition of LGG improves the growth and health of weaned pigs when orally challenged by E. coli F4. Thirty-six pigs were weaned at 21 days and assigned to a standard weaning diet with or without 10 10 CFU LGG (ATCC 53103) per day. The pigs, individually penned, were orally challenged with 1.5 ml of a 10 10 CFU E. coli F4 suspension on day 7 and slaughtered on day 12 or 14. With the addition of LGG, the average daily gain and the average daily feed intake were reduced after the challenge with ETEC and for the entire trial ( P , 0.05). The average faecal score tended to worsen from day 11 to the end of the trial and the concentration of ETEC in the faeces tended to increase ( P 5 0.07) with the LGG supplementation. The counts of lactic acid bacteria, enterobacteria and yeasts in the colonic digesta were not affected. The pH values in ileal, colonic and caecal digesta, and the small intestine size were also unchanged. Regardless of the site of measurement (duodenum, jejunum or ileum), a trend of decreased villus height was seen with LGG ( P 5 0.10). Crypt depth and villus to crypt ratio were unchanged by the diet. A gradual increase of total seric IgA was seen after 1 week and after the challenge, in the control ( P , 0.05), but not in the treated group. After the challenge, the LGG reduced the total IgA in the blood serum ( P , 0.05), v. the control. The total IgA in the saliva and in the jejunum secretion were not affected by the diet. The F4-specific IgA activity was not affected by the diet at all the samplings. Our result shows that, the administration of LGG do not prevent or reduce the detrimental effect of the E. coli F4 infection on the growth performance and health status of weaned piglet.

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“…johnsonii (615) and Lb. plantarum (616,617) residing in the GIT have been reported. These studies illustrate the complex molecular adaptations of these LAB to the many physicochemical challenges encountered during mammalian intestinal tract transit.…”

Section: Applications Of Functional Genomic Technologies In Situmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

Self Cite

472

376

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice

Cited by 92 publications

References 18 publications

A metabolomic view of how the human gut microbiota impacts the host metabolome using humanized and gnotobiotic mice

A metabolomic view of how the human gut microbiota impacts the host metabolome using humanized and gnotobiotic mice

Dietary addition of Lactobacillus rhamnosus GG impairs the health of Escherichia coli F4-challenged piglets

Stress Physiology of Lactic Acid Bacteria

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