Conventional pluripotency markers are unspecific for bovine embryonic-derived cell-lines

Muñoz, M.; Rodríguez, Aida; Frutos, C. de; Caamaño, J. N.; Díez, C.; Facal, N.; Gómez, E.

doi:10.1016/j.theriogenology.2008.02.014

Cited by 70 publications

(53 citation statements)

References 27 publications

(49 reference statements)

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“…Numerous attempts to establish bovine ESC lines have been undertaken; however, they have had limited success (Gong et al, 2010;Jin et al, 2012;Maruotti et al, 2012;Saito et al, 1992;Saito et al, 2003;Solter et al, 2000). Most ESC-like cultures proliferated slowly, lost pluripotency markers, and ceased to divide at early passages (Cao et al, 2009;Malaver-Ortega et al, 2012;Maruotti et al, 2012;Munoz et al, 2008;Saito et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Talluri

Kumar

Glage

et al. 2015

Cellular Reprogramming

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Induced pluripotent stem cells (iPSCs) are a seminal breakthrough in stem cell research and are promising tools for advanced regenerative therapies in humans and reproductive biotechnology in farm animals. iPSCs are particularly valuable in species in which authentic embryonic stem cell (ESC) lines are yet not available. Here, we describe a nonviral method for the derivation of bovine iPSCs employing Sleeping Beauty (SB) and piggyBac (PB) transposon systems encoding different combinations of reprogramming factors, each separated by self-cleaving peptide sequences and driven by the chimeric CAGGS promoter. One bovine iPSC line (biPS-1) generated by a PB vector containing six reprogramming genes was analyzed in detail, including morphology, alkaline phosphatase expression, and typical hallmarks of pluripotency, such as expression of pluripotency markers and formation of mature teratomas in immunodeficient mice. Moreover, the biPS-1 line allowed a second round of SB transposon-mediated gene transfer. These results are promising for derivation of germ linecompetent bovine iPSCs and will facilitate genetic modification of the bovine genome.

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Section: Introductionmentioning

confidence: 99%

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Talluri

Kumar

Glage

et al. 2015

Cellular Reprogramming

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“…In the mouse, SSEA-1 can be detected in the inner cell mass and ESCs, whereas in humans SSEA-1 is absent but the cells are positive for SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 antigens. To add further complexity, in the bovine blastocyst (another related ungulate species), expression of SSEA1, SSEA-4, TRA-1-60, and TRA-1-80 are expressed both in the inner mass and trophoectoderm, suggesting that these markers cannot be used to identify bovine ESCs exclusively (42). There is also dispute concerning the topographic and temporal expression of Sox2, Oct4, and Nanog in porcine and bovine blastocysts (32), which may indicate that unidentified transcription factors play a more relevant role in ungulates.…”

Section: Porcine Ipscs Express Esc Markers and Are Pluripotentmentioning

confidence: 99%

Porcine induced pluripotent stem cells may bridge the gap between mouse and human iPS

et al. 2010

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SummaryRecently, three independent laboratories reported the generation of induced pluripotent stem cells (iPSCs) from pig (Sus scrofa). This finding sums to the growing list of species (mouse, human, monkey, and rat, in this order) for which successful reprogramming using exogenous factors has been achieved, and multiple others are possibly forthcoming. But apart from demonstrating the universality of the network identified by Shinya Yamanaka, what makes the porcine model so special? On one side, pigs are an agricultural commodity and have an easy and affordable maintenance compared with nonhuman primates that normally need to be imported. On the other side, resemblance (for example, size of organs) of porcine and human physiology is striking and because pigs are a regular source of food the ethical concerns that still remain in monkeys are not applicable. Besides, the prolonged lifespan of pigs compared with other domestic species can allow exhaustive follow up of side effects after transplantation. Porcine iPSCs may thus fill the gap between the mouse model, which due to its ease is preferred for mechanistic studies, and the first clinical trials using iPSCs in humans. However, although these studies are relevant and have created significant interest they face analogous problems that we discuss herein together with potential new directions. THE DISCOVERY OF INDUCED PLURIPOTENCYThe isolation of mouse and human embryonic stem cells (ESCs) in 1981 and 1998, respectively, has supposed a tremendous scientific advance (1, 2). The longstanding promise of ESCs has been to replace diseased cells and organs usingunless differentiation is triggered-an unlimited source of selfrenewing cells which have the ability to produce all tissues that compose an adult individual (pluripotency). In addition, ESCs have provided a new and powerful model for studying cell fate determination, epigenetic regulation, and disease. Remarkably, mouse ESCs also initiated mammalian genetic manipulation by means of homologous recombination. However, at least in humans, ESCs have ignited a debate that seriously hampered both clinical application and research (3, 4). To avoid controversy, scientists have pursued the generation of ESC-like cells from terminally differentiated (somatic) cells using varied methods [fusion with ESCs, somatic cell nuclear transfer (SCNT), and iPS] that hereafter are generically referred to as nuclear reprogramming or reprogramming (5, 6).Several decades ago, pioneer experiments by Briggs, King, and Gurdon demonstrated that transfer of the nucleus from a frog somatic cell into an enucleated egg can allow the development of this egg to a heartbeat-stage tadpole (7). These experiments set the arena for intensive research that culminated in 1997 with the birth of Dolly the sheep using SCNT (5). Theoretically, SCNT-derived human blastocysts could also be used to produce patient-specific ESC-like cells less constrained by ethical concerns, but the technique is more challenging in humans than in other species and the scarci...

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“…However, in ungulates, these markers are expressed both within the inner cell mass and the trophectoderm (Fig. 2); therefore their use as single markers might be inappropriate to identify ungulate ESC [17].…”

Section: Species-specific Pluripotency Markersmentioning

confidence: 99%

Constraints to Progress in Embryonic Stem Cells from Domestic Species

Muñoz

Trigal

Molina

et al. 2009

Stem Cell Rev and Rep

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Domestic animal embryonic stem cells are of potentially big value in transgenic research and studies of lineage commitment and development. Unfortunately, despite many efforts, validated embryonic stem cell lines in species other than mice and primates are yet to be isolated. Here we review some factors that might help to explain why derivation of domestic animal embryonic stem cells is still unsuccessful. Keywords Embryonic stem cells . Domestic speciesSince the first embryonic stem cell lines isolation from mouse embryos in 1981 [1, 2] the number of published articles dealing with these unique cells has steadily increased ( Fig. 1. Pubmed citations dated on January 1981-December 2008). The rise in the number of publications can be taken as a reflection of the interest aroused by these cells due to their unique biological properties and numerous applications.Stem cells derived from early embryos (Embryonic Stem Cells; ESC) are cultured cell lines that can be clonally propagated and maintained in culture indefinitely, while their ability to differentiate in vitro or in vivo into derivatives of all three germ layers remains preserved. Thus, ESC can contribute to form all tissues and organs, including germ cells. These singular traits have made ESC a useful tool for studying mammalian development, nuclear reprogramming, and cell lineage commitment and differentiation.Manipulation of ESC by homologous recombination is highly efficient [3], which provides a route for the precise modification of the animal genome by gene targeting. Currently, genetically modified mice are routinely produced by embryonic stem cell technology [4]. Transgenic mice are essential tools in the understanding of functional genomics, mammalian physiology and as animal models for a number of human diseases.Several animal reproductive biology research initiatives have envisaged the production of gene-targeted farm animals for improving productive traits and disease resistance and as models for human diseases. Such farm species would not show some of the limitations of the mouse model i.e. short life span or a physiology and anatomy very different from humans. Unfortunately, contrary to mice and primates, a key barrier to genetic modification in livestock has been the failure to establish embryonic stem cell lines from domestic species, which remains as a multi-faceted challenge. As pointed-out within several recent reviews [5][6][7][8][9], progress in ESC will arise from research focusing on the understanding of species-specific mechanisms that maintain pluripotency; identification of appropriate stem cell markers; and optimization of culture conditions.The existence of significant morphological and functional similarities between ESC from very different species [10,11] might have led researchers to an inappropriate, direct application on farm species of specific conditions used to isolate and maintain ESC from mice and humans. As a result of this misconception, and provided that important differences have also been observed [11], we n...

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Conventional pluripotency markers are unspecific for bovine embryonic-derived cell-lines

Cited by 70 publications

References 27 publications

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Porcine induced pluripotent stem cells may bridge the gap between mouse and human iPS

Constraints to Progress in Embryonic Stem Cells from Domestic Species

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