Conventional PCR and real time quantitative PCR detection of Phytophthora cryptogea on Gerbera jamesonii

Minerdi, Daniela; Moretti, Marino; Li, Yuan; Gaggero, Laura; Garibaldi, A.; Gullino, M. L.

doi:10.1007/s10658-008-9276-3

Cited by 32 publications

(22 citation statements)

References 25 publications

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“…; Minerdi et al. ). Currently, little information on biological thresholds, which is the amount of inoculum present in water associated with subsequent disease development, is available.…”

Section: Detection Of Waterborne Fungi and Oomycetesmentioning

confidence: 98%

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Sanzani

Nicosia

Faedda³

et al. 2013

Journal of Phytopathology

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Quantitative polymerase chain reaction (qPCR) is a versatile technique for the accurate, sensitive, reliable and high‐throughput detection and quantification of target DNA in various environmental samples, and in recent years, it has greatly contributed to the advancement of knowledge in the plant pathology field. Indeed, this technique is ideal to evaluate inoculum threshold levels and to study the epidemiology, biology and ecology of phytopathogenic fungi and oomycetes, thus opening up new research opportunities to investigate host–pathogen interactions and to address tasks related to quarantine, eradication and biosecurity. Moreover, it can be a useful tool in breeding programs. The present review analyses the most relevant applications of qPCR for the detection and quantification of filamentous fungi and oomycetes within host tissues and in soil, air and water, along with brief paragraphs focusing on new application fields such as the detection and quantification of mycotoxigenic fungi and biocontrol agents. The high potentiality of qPCR for present and future applications is highlighted together with a critical analysis of major drawbacks that need to be corrected to definitively confirm it as a preferential routine quantitative detection method.

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“…; Minerdi et al. ). Currently, little information on biological thresholds, which is the amount of inoculum present in water associated with subsequent disease development, is available.…”

Section: Detection Of Waterborne Fungi and Oomycetesmentioning

confidence: 98%

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Sanzani

Nicosia

Faedda³

et al. 2013

Journal of Phytopathology

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“…The effects of soil fumigants on the control of FWD mostly relies on quantifying Fusarium pathogens by either isolating and quantifying them on selective medium or detecting Fusarium DNA by semi‐quantitative PCR . Recently, a real‐time PCR‐based technique has been described that enables calculation of the amount of a PCR product present in a sample during the exponential phase of DNA amplification …”

Section: Introductionmentioning

confidence: 99%

Quantification of Fusarium oxysporum in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants

Mao

Yan

et al. 2014

Pest. Manag. Sci.

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“…Similar to the previous work, the primers used in the MELGA technique displayed high specificity for the leukemic K562 cell line ( BCR/ABL expression). The detection limit for this technique is <1 fg/ml of RNA in the K562 cell line, which is lower than RQ‐PCR , conventional PCR , and ELISA (i.e., picogram, nanogram, and nanogram, respectively). At <1 fg/ml of total RNA, the OD was slightly increased.…”

Section: Discussionmentioning

confidence: 87%

Magnetic Nanoparticles PCR Enzyme‐Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia

Manthawornsiri

Polpanich

Yamkamon

et al. 2015

Clinical Laboratory Analysis

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Conventional PCR and real time quantitative PCR detection of Phytophthora cryptogea on Gerbera jamesonii

Cited by 32 publications

References 25 publications

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Quantification of Fusarium oxysporum in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants

Magnetic Nanoparticles PCR Enzyme‐Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia

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