Quantification of <i>Fusarium oxysporum</i>
 in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants

Li, Yuan; Mao, Liangang; Yan, Dongdong; Ma, Taotao; Shen, Jiahao; Guo, Ming; Wang, Qiuxia; Ouyang, Canbin; Cao, Aocheng

doi:10.1002/ps.3700

Cited by 36 publications

(25 citation statements)

References 23 publications

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“…The other tested fumigant metam sodium also induced a significant reduction in the population of F. oxysporum, which lasted for 60 days. Our data are in agreement with findings by Li et al (2014) who showed that DMDS, when used alone or in combination with chloropicrin, substantially reduced the population of F. oxysporum with reduction levels of 54-58 % (DMDS), 77-79 % ( D M D S : C h l o r o p i c r i n 2 : 1 ) a n d 8 7 -8 8 % (DMDS:Chloropicrin 1:2). In similar studies the application of DMDS alone or in combination with dazomet ), or chloropicrin (Basallote-Ureba et al 2010 significantly reduced the incidence of F. oxysporum in cucumber and carnation plants respectively.…”

Section: Impact Of Fumigants On F Oxysporumsupporting

confidence: 95%

“…With the exception of the recent study by Li et al (2014) all other studies that have investigated the impact of fumigants including DMDS on the population dynamics of soil borne fungal pathogens they have utilized cultivation-based methods (Singleton et al 1992). The latter are time-consuming and do not provide an accurate estimation of the fungal population due to the proven limited selectivity of the growth media used (Thorn et al 1996;Termorshuizen et al 1998).…”

Section: Introductionmentioning

confidence: 98%

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Assessment of the impact of the fumigant dimethyl disulfide on the dynamics of major fungal plant pathogens in greenhouse soils

et al. 2016

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Soil fumigants constitute a major tool for the control of soil borne fungal plant pathogens in protected crops. Dimethyl disulfide (DMDS) is a novel soil fumigant used either alone or in combination with other fumigants for the control of soil borne pests and diseases. In a commercial greenhouse for tomato production, we evaluated the impact of DMDS, comparatively to the alternative fumigant metam sodium, on the population of the dominant fungal plant pathogens in the study soil via q-PCR. Prior to soil fumigation, estimation of the fungal diversity in the studied soil via clone libraries identified Fusarium oxysporum and Rhizoctonia solani as the most abundant soil borne plant pathogens, while Cladosporium spp., known as opportunistic airborne tomato pathogens, were the most dominant fungi and based on this their dynamics upon fumigation was also studied. DMDS, at two dose rates, induced a drastic reduction in the population of F. oxysporum and R. solani, which lasted for the whole cultivation season. On the contrary, metam sodium exhibited an inhibitory effect on F. oxysporum that was alleviated at 120 d post fumigation. Both DMDS and metam sodium induced only a temporal reduction in the soil population of Cladosporium sp. which recovered by 60 days post fumigation. Our data suggest that DMDS even at the low dose rate (56.4 g m −2 ) could drastically reduce the population of the major soil borne tomato pathogens F. oxysporum and R. solani. Establishment of population thresholds as determined by q-PCR could represent a valuable tool for the estimation of the risk for disease severity and crop yield losses.

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Section: Impact Of Fumigants On F Oxysporumsupporting

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Assessment of the impact of the fumigant dimethyl disulfide on the dynamics of major fungal plant pathogens in greenhouse soils

et al. 2016

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“…lycopersici , but the expression of the disease was not prevented in lettuce ( Lactuca sativa L.). Similarly, Li et al . reported that DMDS + Pic was more effective than DMDS alone and successfully suppressed the incidence of Fusarium wilt in strawberry ( Fragaria × ananessa Duchesne).…”

Section: Resultsmentioning

confidence: 90%

“…DMDS primarily affects mitochondrial respiration through blocking the activity of the enzyme cytochrome oxidase . DMDS has proven to be an effective fumigant for control of Cyperus spp . and other soilborne pathogens such as Fusarium oxysporum and Meloidogyne nematodes .…”

Section: Introductionmentioning

confidence: 99%

Pest control with drip‐applied dimethyl disulfide and chloropicrin in plastic‐mulched tomato (Solanum lycopersicum L.)

Sharpe

Vallad

et al. 2019

Pest Management Science

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BACKGROUND Dimethyl disulfide (DMDS) is used as a preplant soil fumigant for weed and soilborne pathogen control in plasticulture vegetable crops. The objective of this research was to determine the control efficacy of emulsifiable concentrate (EC) formulation of DMDS or DMDS + chloropicrin (Pic) on weed and Fusarium wilt in tomato (Solanum lycopersicum L.) plasticulture. RESULTS The effective DMDS rates required to provide 50% (ER50) control of purple nutsedge (Cyperus rotundus L.) were 210 and 340 kg ha−1 at 4 weeks after fumigation (WAF) in fall 2017 and fall 2018, respectively, while these values increased to 348 and >467 kg ha−1, respectively, at 12 WAF. The ER50 values of DMDS + Pic were 150 and 240 kg ha−1 at 4 WAF in fall 2017 and fall 2018, respectively, while these values increased to 255 and 450 kg ha−1, respectively, at 12 WAF. DMDS + Pic was generally more effective than DMDS for C. rotundus control. The high rates of DMDS or DMDS + Pic provided adequate C. rotundus control in early season but failed to provide effective control by season end. In addition, DMDS + Pic injections through drip tape effectively reduced Fusarium oxysporum f. sp. lycopersici (FOL) inoculum while DMDS alone was generally ineffective. CONCLUSION Injection of the EC formulation of DMDS or DMDS + Pic through drip tape should have been provided a viable option for C. rotundus and Fusarium wilt control in plastic‐mulched tomato. However, supplemental weed management actions, such as herbicide applications, may be required to achieve season‐long control. © 2019 Society of Chemical Industry

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“…The assay was also successfully applied for the qualitative and quantitative analysis of Fusarium species in contaminated maize samples . Li et al conducted a real‐time PCR assay based on the FOW1 gene encoding a mitochondrial carrier protein for detection and quantification of F. oxysporum , by which a strong positive correlation was found between the copy number (×10) of the F. oxysporum FOW1 gene in 5 g of soil and the colony forming unit (CFU) number . Furthermore, a DNA microarray for easy and fast detection of trichothecene‐ and moniliformin‐producing Fusarium species has been developed, which has the capture probes designed by the tef‐1α sequence.…”

Section: The Use Of Polymerase Chain Reaction For Detecting Fusarium mentioning

confidence: 99%

Molecular strategies for detection and quantification of mycotoxin‐producing Fusarium species: a review

2014

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Fusarium contamination is considered a major agricultural problem, which could not only significantly reduce yield and quality of agricultural products, but produce mycotoxins that are virulence factors responsible for many diseases of humans and farm animals. One strategy to identify toxigenic Fusarium species is the use of modern molecular methods, which include the analysis of DNA target regions for differentiation of the Fusarium species, particularly the mycotoxin-producing Fusarium species such as F. verticillioides and F. graminearum. Additionally, polymerase chain reaction assays are used to determine the genes involved in the biosynthesis of the toxins in order to facilitate a qualitative and quantitative detection of Fusarium-producing mycotoxins. Also, it is worth mentioning that some factors that modulate the biosynthesis of mycotoxins are not only determined by their biosynthetic gene clusters, but also by environmental conditions. Therefore, all of the aforementioned factors which may affect the molecular diagnosis of mycotoxins will be reviewed and discussed in this paper.

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Quantification of Fusarium oxysporum in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants

Cited by 36 publications

References 23 publications

Assessment of the impact of the fumigant dimethyl disulfide on the dynamics of major fungal plant pathogens in greenhouse soils

Assessment of the impact of the fumigant dimethyl disulfide on the dynamics of major fungal plant pathogens in greenhouse soils

Pest control with drip‐applied dimethyl disulfide and chloropicrin in plastic‐mulched tomato (Solanum lycopersicum L.)

Molecular strategies for detection and quantification of mycotoxin‐producing Fusarium species: a review

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