Conventional methods and future trends in antimicrobial susceptibility testing

Salam, Md. Abdus; Al-Amin, M.; Pawar, Jogendra Singh; Akhter, Naseem; Lucy, Irine Banu

doi:10.1016/j.sjbs.2023.103582

Cited by 42 publications

(40 citation statements)

References 54 publications

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“…CaF 2 nanoparticles are water‐insoluble, and their minimal inhibitory concentration (MIC) is determined by the turbidimetric assay method as shown in Figure 4 20,25 . The test is carried out against two‐gram negative bacteria E .…”

Section: Resultsmentioning

confidence: 99%

“…The generated colonies were measured after these LB agar plates had been incubated at 37 C overnight. 20…”

Section: Methods Of Determining Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…To determine the minimal inhibitory concentration of CaF 2 Nps, 100 mL of aliquots from tubes were drawn and plated on LB agar plates. The generated colonies were measured after these LB agar plates had been incubated at 37°C overnight 20 …”

Section: Methodsmentioning

confidence: 99%

“…CaF 2 nanoparticles are water-insoluble, and their minimal inhibitory concentration (MIC) is determined by the turbidimetric assay method as shown in Figure 4. 20,25 The test is carried out against two-gram negative bacteria E. coli, Pseudomonas aeruginosa, and two-gram positive bacteria strain Bacillus badius, S. aureus. The procedure of estimating the value of MIC is pictorially represented in Figure 4.…”

Section: Antibacterial Activity Of Caf 2 Npsmentioning

confidence: 99%

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Fabrication of nano calcium fluoride impregnated organic polyester alkyd coating for anticorrosive and antibacterial applications

Benitha,

Jeyasubramanian,

Vijayakumari

et al. 2023

J of Applied Polymer Sci

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The advent of organic polyester alkyd resin has revolutionized the coating industry due to its good adherence to any substrate, high gloss, and greater drying speed. This work deals with the characterization of nano calcium fluoride (CaF2 Nps) incorporated polyester alkyd coating for efficient multifunctional coating applications. The CaF2 Nps are prepared by employing a facile co‐precipitation process. The prepared particles are characterized using X‐ray diffraction analysis (XRD) and scanning electron microscopy (SEM) to analyze their size and morphology. By employing the ball milling technique, the CaF2 Nps along with other additives are mixed with alkyd resin to form a coating. The spreading ability, volatile and non‐volatile content, surface roughness, and coating thickness are evaluated. The minimal inhibitory concentration (MIC) is estimated by the turbidimetric assay method against gram‐negative and gram‐positive bacteria strains. Since these pigments hold very good antibacterial properties, the CaF2 are impregnated in alkyd resin coating, in which CaF2 nanoparticles are used as pigments. To reduce the nosocomial infection spreading in the hospital environment the formulated paint is analyzed for its antibacterial and anticorrosive studies. The current study confirmed CaF2 impregnated organic alkyd resin coating possesses promising comparable properties which would be a good choice for coatings, addressing multifunctional properties.

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Section: Resultsmentioning

confidence: 99%

“…The generated colonies were measured after these LB agar plates had been incubated at 37 C overnight. 20…”

Section: Methods Of Determining Minimum Inhibitory Concentrationmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Antibacterial Activity Of Caf 2 Npsmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of nano calcium fluoride impregnated organic polyester alkyd coating for anticorrosive and antibacterial applications

Benitha,

Jeyasubramanian,

Vijayakumari

et al. 2023

J of Applied Polymer Sci

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“…The broth microdilution method is a fast and accurate assay that utilizes small volumes of test antimicrobial and allows for rapid testing of bacteria. Nonetheless, the process of manually handling solutions of antimicrobial agents during the preparation stage may increase the possibility of errors (Salam et al., 2023). Agar dilution methods offer several advantages when compared to diffusion techniques, including the ability to test multiple biological isolates simultaneously, the capacity to observe heterogeneous populations or mixed cultures, and the flexibility to select a range of sample concentrations for testing (Salam et al., 2023).…”

Section: Resultsmentioning

confidence: 99%

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

Ezzaky,

Elmoslih,

Silva

et al. 2023

Comp Rev Food Sci Food Safe

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Essential oils (EOs) are a class of natural products that exhibit potent antimicrobial properties against a broad spectrum of bacteria. Inhibition diameters (IDs) and minimum inhibitory concentrations (MICs) are the typical measures of antimicrobial activity for extracts and EOs obtained from Cinnamomum, Salvia, and Mentha species. This study used a meta‐analytical regression analysis to investigate the correlation between ID and MIC measurements and the variability in antimicrobial susceptibility tests. By utilizing pooled ID models, this study revealed significant differences in foodborne pathogens’ susceptibility to extracts, which were dependent on both the plant species and the methodology employed (p < .05). Cassia showed the highest efficacy against Salmonella spp., exhibiting a pooled ID of 26.24 mm, while cinnamon demonstrated the highest efficacy against Bacillus cereus, with a pooled ID of 23.35 mm. Mint extract showed the greatest efficacy against Escherichia coli and Staphylococcus aureus. Interestingly, cinnamon extract demonstrated the lowest effect against Shiga toxin‐producing E. coli, with a pooled ID of only 8.07 mm, whereas its EOs were the most effective against this bacterial strain. The study found that plant species influenced the MIC, while the methodology did not affect MIC measurements (p > .05). An inverse correlation between ID and MIC measurements was identified (p < .0001). These findings suggest that extracts and EOs obtained from Cinnamomum, Salvia, and Mentha spp. have the potential to inhibit bacterial growth. The study highlights the importance of considering various factors that may influence ID and MIC measurements when assessing the effectiveness of antimicrobial agents.

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The importance, benefits, and future of nanobiosensors for infectious diseases

Dhahi,

Dafhalla,

Saad

et al. 2024

Biotech and App Biochem

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Infectious diseases, caused by pathogenic microorganisms such as bacteria, viruses, parasites, or fungi, are crucial for efficient disease management, reducing morbidity and mortality rates and controlling disease spread. Traditional laboratory‐based diagnostic methods face challenges such as high costs, time consumption, and a lack of trained personnel in resource‐poor settings. Diagnostic biosensors have gained momentum as a potential solution, offering advantages such as low cost, high sensitivity, ease of use, and portability. Nanobiosensors are a promising tool for detecting and diagnosing infectious diseases such as coronavirus disease, human immunodeficiency virus, and hepatitis. These sensors use nanostructured carbon nanotubes, graphene, and nanoparticles to detect specific biomarkers or pathogens. They operate through mechanisms like the lateral flow test platform, where a sample containing the biomarker or pathogen is applied to a test strip. If present, the sample binds to specific recognition probes on the strip, indicating a positive result. This binding event is visualized through a colored line. This review discusses the importance, benefits, and potential of nanobiosensors in detecting infectious diseases.

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Conventional methods and future trends in antimicrobial susceptibility testing

Cited by 42 publications

References 54 publications

Fabrication of nano calcium fluoride impregnated organic polyester alkyd coating for anticorrosive and antibacterial applications

Fabrication of nano calcium fluoride impregnated organic polyester alkyd coating for anticorrosive and antibacterial applications

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

The importance, benefits, and future of nanobiosensors for infectious diseases

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