Convention on nomenclature for DNA cytometry

Abstract

Analysis of cellular DNA content by cytometry is important in clinical and biological research. Measurements are used widely to assess the relative DNA content of tumor stemlines and to assist in the detection and evaluation of malignant diseases. A review of the literature on DNA measurements in solid tumor and leukemias reveals a confusing variety of terms applied for the description of similar results. In order to facilitate the understanding of data and to standardize the terminology for DNA analysis, a questionnaire was distributed to more than 500 investigators. Subsequently, a workshop on terminology was held at the Combined

DNA content and MHC class II antigen expression in malignant melanoma: clinical course.

“…DNA ploidy was expressed by a DNA index calculated as the ratio of the mode of the aneuploid peak to the diploid peak. 24 Although the number ofcells in the S + G2M phases of the cell cycle were similar to those found by other investigators, calculation of a proliferative index from computer fitted curves was considered too inaccurate for attempted correlation with other measurements in this study. IMMUNOHISTOLOGY Sections (5ym) from the same blocks of paraffin wax embedded tissue were stained for MHC class II antigen and common leucocyte antigen using the alkaline phosphatase antialkaline phosphatase (APAAP) technique.25 After routine dewaxing of slides and rehydration in descending concentrations of alcohol, samples were incubated for one hour at room temperature with a 1:10 dilution of monoclonal antibody CR3/43, which reacts with a monomorphic determinant of the class II molecules DR, DP, and DQ.26 Analysis of the expression of the individual sublocus products was precluded because we had no sublocus specific antibodies capable of staining archival material.…”

So-Called Congenital-Infantile Fibrosarcoma: Does It Exist and What Is It?

“…Aneuploidy was defined according to criteria defined by Hiddemann et al (14). The percentage of nuclei in the Sphase fraction (SPF) was used as an indicator of proliferation activity.…”

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“…There was a family history ofbreast cancer in 21.6% of cases. In the group of operated patients, 33.3% had pT3 tumours and 53.3% had axillary lymph node metastases. All but one tumour were invasive ductal carcinomas; the remaining was an invasive papillary carcinoma.…”