Convenient enzymatic synthesis of a p-nitrophenyl oligosaccharide series of sialyl N-acetyllactosamine, sialyl Lex and relevant compounds

A novel transglycosylating β-galactosidase from Enterobacter cloacae B5

“…Although bgalactosidases from numerous microorganisms have been used to produce prebiotic GOS, few of them have been applied in synthesizing additional chemicals. Most synthetic work was focused on two microbial b-galactosidases: one from Bacillus circulans and the other from Aspergillus oryzae [11][12][13][14][15][16].…”

Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens

“…[10][11][12][13][14] A limited number of structures have been synthesized in small amounts using glycosyltransferases as an alternative approach to the chemical synthesis. [15][16][17][18] In this work, we developed an efficient enzymatic strategy to produce 24 pLN derivatives in gram-scale amounts to supply our glycan compound library (www.functionalglycomics.org). We have taken advantage of the relaxed substrate specificity of the recombinant bacterial enzymes b-(1!4)-galactosyltransferase (b4GalT; EC 2.4.1.38) and b-(1!3)-N-acetylglucosaminyltransferase (b3GlcNAcT; EC 2.4.1.149), which, by concerted action, generate repeating LN units.…”

Causes of the Production of Multiple Forms of β-Galactosidase byBacillus circulans

“…4,5,12,13) In particular, -galactosidase-2 does not require high lactose concentrations to produce high yields of oligosaccharides, in contrast to most -galactosidases reported to date. 4) -Galactosidase-2 produces GOS at a yield of about 41% from a 4.56% solution of lactose at 40 C. Hence, during the past 20 years, the commercial enzyme Biolacta Ò has frequently been utilized to synthesize various functional galactosylated compounds [32][33][34][35][36][37][38][39][40][41][42] as well as GOS. 5,9,12,13,43,44) In a later study, Vetere et al 45) isolated a third enzyme, with a molecular mass of 86 kDa, from Biolacta Ò , but they did not study oligosaccharide production.…”