Controls on the rate of ureolysis and the morphology of carbonate precipitated by S. Pasteurii biofilms and limits due to bacterial encapsulation

Cuthbert, Mark O.; Riley, Michael S.; Handley-Sidhu, Stephanie; Renshaw, Joanna C.; Tobler, Dominique J.; Phoenix, Vernon R.; Mackay, R.

doi:10.1016/j.ecoleng.2012.01.008

Cited by 98 publications

(93 citation statements)

References 15 publications

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“…8, the resulting model fit to the observed calcium data is only slightly reduced with normalised root-mean-square deviations of 4-7 %. This confirms that the single reaction model may be adequate in such cases for simulating carbonate precipitation, as previously suggested by (Van Paassen 2009;Cuthbert et al 2012).…”

Section: Urease-active Jack Bean Meal Batch Experimentssupporting

confidence: 73%

“…S. pasteurii) adding nutrients will not be necessary (Tobler et al 2011). However, research by Cuthbert et al (2012) has found that ureolysis is limited by the eventual encapsulation of ureolytic bacteria and biofilm by calcite. This would mean that for sealing large voids (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…Calcite precipitation technologies (CPT) have a wide range of current and potential applications, including solid-phase capture and remediation of problematic trace metals and radionuclides (Curti 1999;Warren et al 2001;Fujita et al 2004;, remediation of cracked concrete (Whiffin et al 2007;De Muynck et al 2008;Ghosh et al 2009), durability of concrete structures (Ramachandran et al 2001;De Muynck et al 2010;, treatment of urea from wastewaters (Hammes et al 2003), carbon sequestration (White et al 2003;Druckenmiller et al 2006;Cunningham et al 2009;Mitchell et al 2010), soil improvement (Whiffin 2004) and sealing porosity or rock fractures (Ferris et al 1996;Cunningham et al 2009;Cuthbert et al 2012;Tobler et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Kinetics of urease mediated calcite precipitation and permeability reduction of porous media evidenced by magnetic resonance imaging

Handley-Sidhu

Sham

Cuthbert

et al. 2013

Int. J. Environ. Sci. Technol.

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The enzyme urease drives the hydrolysis of urea leading to the release of ammonium ions and bicarbonate; in the presence of calcium, the rise in pH leads to increased calcium carbonate saturation and the subsequent precipitation of calcite. Although such alkalinizing ureolysis is widespread in nature, most studies have focussed on bacteria (i.e. indigenous communities or urease-active Sporosarcina pasteurii) for calcite precipitation technologies. In this study, urease-active jack bean meal (from the legume Canavalia ensiformis) was used to drive calcite precipitation. The rates of ureolysis (k urea ), determined from measured NH 4? , enabled a direct comparison to microbial ureolysis rates reported in literature. It is also demonstrated that a simple single reaction model approach can simulate calcite precipitation very effectively (3-6 % normalised root-mean-square deviation). To investigate the reduction of permeability in porous media, jack bean meal (0.5 g L -1) and solutions (400 mM urea and CaCl 2 ) were simultaneously pumped into a borosilicate bead column. One-dimensional magnetic resonance profiling techniques were used, non-invasively, for the first time to quantify the porosity changes following calcite precipitation. In addition, two-dimensional slice selective magnetic resonance images (resolution of *0.5 9 1.0 mm) revealed that the exact location of calcite deposition was within the first 10 mm of the column. Column sacrifice and acid digestion also confirmed that 91.5 % of calcite was located within the first 14 mm of the column. These results have important implications for the design of future calcite precipitation technologies and present a possible alternative to the well known bacterial approaches.

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Section: Urease-active Jack Bean Meal Batch Experimentssupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kinetics of urease mediated calcite precipitation and permeability reduction of porous media evidenced by magnetic resonance imaging

Handley-Sidhu

Sham

Cuthbert

et al. 2013

Int. J. Environ. Sci. Technol.

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“…It is therefore necessary to consider other factors that might have accelerated CaCO 3 precipitation in the presence of non-ureolytic bacteria. Previous studies have shown that precipitated CaCO 3 encapsulates S. pasteurii cells (Castanier et al, 1999;Stocks-Fischer et al, 1999;Mitchell and Ferris, 2006;Dupraz et al, 2009;Cuthbert et al, 2012); it is assumed that the electronegativity of the bacterial cell wall favors the adsorption of cations, such as calcium ions, thus facilitating the CaCO 3 precipitation process on the cell wall (Schultze-Lam et al, 1996). In our experiment, the non-ureolytic bacterium, B. subtilis, exhibited a significantly higher growth rate (Fig.…”

Section: Caco 3 Precipitationmentioning

confidence: 50%

Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Gat

Tsesarsky

Shamir³

et al. 2014

Biogeosciences

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Abstract. Microbial-induced CaCO 3 precipitation (MICP) is an innovative technique that harnesses bacterial activity for the modification of the physical properties of soils. Since stimulation of MICP by urea hydrolysis in natural soils is likely to be affected by interactions between ureolytic and non-ureolytic bacteria, we designed an experiment to examine the interactions between ureolytic and non-ureolytic bacteria and the effect of these interactions on MICP. An artificial groundwater-based rich medium was inoculated with two model species of bacteria, the ureolytic species Sporosarcina pasteurii and the non-ureolytic species Bacillus subtilis. The control treatment was inoculated with a pure culture of S. pasteurii. The following parameters were monitored during the course of the experiment: optical density, pH, the evolution of ammonium, dissolved calcium and dissolved inorganic carbon. The results showed that dissolved calcium was precipitated as CaCO 3 faster in the mixed culture than in the control, despite less favorable chemical conditions in the mixed culture, i.e., lower pH and lower CO 2− 3 concentration. B. subtilis exhibited a considerably higher growth rate than S. pasteurii, resulting in higher density of bacterial cells in the mixed culture. We suggest that the presence of the non-ureolytic bacterial species, B. subtilis, accelerated the MICP process, via the supply of nucleation sites in the form of non-ureolytic bacterial cells.

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“…This thick layer of crystals would reduce the diffusion rate of urea to bacteria. As suggested by Cuthbert et al (2012), the growth of crystals on the surface of bacterial cells encapsulates the cells leading to a reduction in the ureolysis rate. The loss of urease activity due to encapsulation of crystals was commonly found during the MICP process and van Paassen (2009) suggested that the decrease in the bacterial urease activity might be represented by an exponential decay function.…”

Section: Effect Of Concentration Of Cementation Solution On Specific mentioning

confidence: 99%

Urease active bioslurry: a novel soil improvement approach based on microbially induced carbonate precipitation

2016

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This paper presents a novel approach for soil stabilization by microbially induced carbonate precipitation (MICP) using a new urease active catalyzer, named herein as "bioslurry". The bioslurry, which was produced from the reaction between bacterial culture and 400 mmol/L of CaCl 2 and urea, is pre-formed urease active crystals consisting of CaCO 3 plus imbedded urease active bacterial cells. By mixing the bioslurry with sand, more than 95% of the bioslurry was retained in the soil matrix as a result of the mechanical trapping mechanism, leading to high resistance to flushing with a low-salinity solution. The retained urease activity of bioslurry was uniformly distributed along the sand matrix, resulting in a rather uniform CaCO 3 precipitation. Through repeated treatments with a cementation solution, the unconfined compressive strength of bioslurry treated sand was significantly improved due to the effective CaCO 3 precipitation at the contact points of soil grains. Scanning electron microscopy analysis carried out on the bioslurry treated sand revealed that the induced large rhombohedral CaCO 3 crystals were localized around the bioslurry spherical fine crystals. The overall outcome of this work is that soil biocementation using the new bioslurry approach is controllable, reproducible, and homogeneous.Key words: bioslurry, microbially induced carbonate precipitation (MICP), biocementation, ground improvement, soil stabilization.Résumé : Cet article présente une approche novatrice pour la stabilisation des sols par précipitation des carbonates induite par voie microbienne (microbially induced carbonate precipitation, MICP) en utilisant un nouveau catalyseur actif uréase, appelée ici la « boue-bio ». La boue-bio qui a été produite de la réaction entre la culture bactérienne et 400 mmol/L de CaCl 2 et de l'urée, est composée de cristaux actifs uréase préformée consistant en CaCO 3 ainsi que des cellules bactériennes actives uréases incrustées. En mélangeant la boue-bio avec du sable, plus de 95 % de la boue-bio a été maintenue dans la matrice du sol en raison du mécanisme de piégeage mécanique, conduisant à une haute résistance au rinçage d'une solution à faible salinité. L'activité uréase retenue de la boue-bio a été uniformément répartie le long de la matrice de sable, ce qui entraîne une précipitation assez uniforme de CaCO 3 . Grâce à des traitements répétés avec une solution de cimentation, la résistance en compression non confinée de sable traité à la boue-bio a été significativement améliorée en raison des précipitations en CaCO 3 efficaces au niveau des points de contact des grains de sol. L'analyse par microscopie électronique à balayage du sable traité à la boue-bio a révélé que les grands cristaux induits de CaCO 3 rhomboédriques ont été localisés autour des fins cristaux sphériques de la boue-bio. Le résultat global de ce travail est que la biocimentation du sol en utilisant la nouvelle approche de boue-bio est contrôlable, reproductible et homogène. [Traduit par la Rédaction] Mots-clés : bo...

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Controls on the rate of ureolysis and the morphology of carbonate precipitated by S. Pasteurii biofilms and limits due to bacterial encapsulation

Cited by 98 publications

References 15 publications

Kinetics of urease mediated calcite precipitation and permeability reduction of porous media evidenced by magnetic resonance imaging

Kinetics of urease mediated calcite precipitation and permeability reduction of porous media evidenced by magnetic resonance imaging

Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Urease active bioslurry: a novel soil improvement approach based on microbially induced carbonate precipitation

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Controls on the rate of ureolysis and the morphology of carbonate precipitated by S. Pasteurii biofilms and limits due to bacterial encapsulation

Cited by 98 publications

References 15 publications

Kinetics of urease mediated calcite precipitation and permeability reduction of porous media evidenced by magnetic resonance imaging

Kinetics of urease mediated calcite precipitation and permeability reduction of porous media evidenced by magnetic resonance imaging

Accelerated microbial-induced CaCO&lt;sub&gt;3&lt;/sub&gt; precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Urease active bioslurry: a novel soil improvement approach based on microbially induced carbonate precipitation

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Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria