Controlling Pseudomonas aeruginosa infection in Oreochromis niloticus spawners by cefotaxime sodium

Ali, Nadia Gabr; Ali, Tamer El-Sayed; Aboyadak, Ibrahim Mohamed; Elbakry, Mohamed Ahmed

doi:10.1016/j.aquaculture.2021.737107

Cited by 21 publications

(16 citation statements)

References 44 publications

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“…Many researchers [ 18 – 20 ], , have described nearly similar disease characteristics in many fish species infected with P. putida . Virulence factors are the key to understanding bacterial pathogenesis in fishes; they are responsible for the clinical features and disease severity [ 9 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Identification and florfenicol-treatment of pseudomonas putida infection in gilthead seabream (Sparus aurata) fed on tilapia-trash-feed

Aboyadak,

Abdel-Tawwab,

Ali

2024

BMC Vet Res

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The present study aimed to determine the major cause of the high mortality affecting farmed gilthead seabream (Sparus aurata) and controlling this disease condition. Fifteen diseased S. aurata were sampled from a private fish farm located at Eldeba Triangle, Damietta, fish showed external skin hemorrhages, and ulceration. Bacterial isolates retrieved from the diseased fish were identified biochemically as Pseudomonas putida and then confirmed by phylogenetic analysis of the 16 S rRNA gene sequence. P. putida was also isolated from three batches of tilapia-trash feed given to S. aurata. Biofilm and hemolytic assay indicated that all P. putida isolates produced biofilm, but 61.11% can haemolyse red blood cells. Based on the antibiotic susceptibility test results, P. putida was sensitive to florfenicol with minimum inhibitory concentrations ranging between 0.25 and 1.0 µg mL− 1, but all isolates were resistant to ampicillin and sulfamethoxazole-trimethoprim. Pathogenicity test revealed that P. putida isolate (recovered from the tilapia-trash feed) was virulent for S. aurata with LD50 equal to 4.67 × 107 colony forming unit (CFU) fish− 1. After intraperitoneal (IP) challenge, fish treated with 10 mg kg− 1 of florfenicol showed 16.7% mortality, while no mortality was recorded for the fish group that received 20 mg kg− 1. The non-treated fish group showed 46.7% mortality after bacterial challenge. HPLC analysis of serum florfenicol levels reached 1.07 and 2.52 µg mL− 1 at the 5th -day post-drug administration in the fish groups received 10 and 20 mg kg− 1, respectively. In conclusion, P. putida was responsible for the high mortality affecting cultured S. aurata, in-feed administration of florfenicol (20 mg kg− 1) effectively protected the challenged fish.

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Section: Discussionmentioning

confidence: 99%

“…Each feed sample contained 100 g of chopped tilapia. Diseased fish and tilapia-trash feed samples were stored in the car refrigerator (− 8 °C) and then immediately transported to the laboratory as mentioned by El-Bahar et al [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Identification and florfenicol-treatment of pseudomonas putida infection in gilthead seabream (Sparus aurata) fed on tilapia-trash-feed

Aboyadak,

Abdel-Tawwab,

Ali

2024

BMC Vet Res

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“…Bacterial isolation was performed as described by Ali et al. (2021); small slices from the hepatopancreas and posterior kidney of diseased fish (showing signs of septicemia) were taken and incubated in tryptic soy broth at 37°C for 24 h. Marine agar plates were streaked from tryptic soy broth and incubated at 37°C for 24 h.…”

Section: Methodsmentioning

confidence: 99%

The role of Aeromonas genotyping in virulence for Dicentrarchus labrax

Aboyadak,

Soliman,

Nageeb

et al. 2023

Journal of Fish Diseases

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Aeromonas septicemia still represents a serious challenge facing the global aquaculture sector. In the present study, Aeromonas caviae and A. veronii were isolated from four diseased European seabass (Dicentrarchus labrax) farms experiencing a high mortality rate. Diseased fish showed haemorrhages on the external body surface with exophthalmia, cataracts, scale desquamation, skin ulcers and fin erosions. The most common post‐mortem findings were congested internal organs, particularly the liver and posterior kidney. Twenty‐eight A. Veronii and 11 A. caviae isolates were identified biochemically by the Vitek 2 system and then confirmed by PCR and phylogenetic analysis. Hemolysin (hlyA) and aerolysin (aer) were the most abundant virulence genes in the recovered isolates, followed by cytotoxic enterotoxin (act) and heat‐stable enterotoxin (ast). A. caviae was more virulent than A. veronii for D. labrax fingerlings as LD50 ranging between (>1 × 108–6.2 × 107) for A. veronii and (2.9 × 107–8.3 × 107) for A. caviae. The sensitivity test indicated the effectiveness of norfloxacin, doxycycline and oxytetracycline against the tested isolates. Serum cortisol significantly increased in the infected groups, while catalase and glutathione peroxidase activities significantly decreased at 2 days post‐infection (DPI) and then increased at 6 DPI. The presence of virulence genes was associated with bacterial pathogenicity expressed in fish mortality rate. Virulence genes also drastically affect cortisol levels more than catalase and glutathione peroxidase levels.

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“…Satu jenis ikan (Labeo rohita) yang diberi pakan mengandung heat-killed Pseudomonas aeruginosa dilaporkan menunjukkan peningkatan parameter daya tahan tubuh non spesifik dan sintasan yang lebih tinggi ketika diinfeksi dengan Aeromonas hydrophila (Giri et al, 2020). Namun demikian, terdapat strain Pseudomonas aeruginosa dapat menyebabkan penyakit pada ikan (Baldissera et al, 2019;Ali et al, 2021). Infeksi Pseudomonas aeruginosa pada ikan nila (Oreochromis niloticus) menimbulkan gejala seperti bintik kemerahan, sirip geripis, nekrosis pada insang, hati berwarna pucat, serous fluid exudate, dan congested kidney (Algammal et al, 2020) (Gao et al, 2019) dan menyebabkan pertumbuhan lambat pada udang galah dewasa (Gao et al, 2021).…”

Section: Pembahasanunclassified

Isolasi dan Identifikasi Molekuler Bakteri Proteolitik dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) yang Dibudidayakan di Kabupaten Banyumas

Nurhafid

Syakuri

Oedjijono

et al. 2021

JFS

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Keberadaan bakteri proteolitik pada komoditas akuakultur penting untuk dipelajari, salah satunya terkait dengan praktek budidaya ikan skala kecil di daerah pedesaan. Penelitian ini bertujuan untuk mengetahui keberadaan dan melakukan identifikasi secara molekuler bakteri proteolitik yang diisolasi dari saluran pencernaan ikan nila (Oreochromis niloticus). Sampel ikan nila diambil dari tiga unit kegiatan akuakultur yang menggunakan pakan berbeda di Kabupaten Banyumas yaitu dari Desa Pandak (dengan probiotik, pakan pellet), Desa Beji (tanpa probiotik, pakan tumbuhan) dan Desa Tambaksogra (dengan probiotik, kombinasi pakan pellet dan tumbuhan). Jumlah bakteri, proporsi bakteri proteolitik, dan indeks aktifitas proteolitik diamati dari usus bagian anterior, middle, dan posterior. Sampel isolat bakteri proteolitik dikelompokkan berdasarkan hasil analisis restriksi 16S rDNA menggunakan software PhyElp. Bakteri dari setiap kelompok diidentifikasi berdasarkan sekuen gen 16S rDNA dengan menggunakan analisis BLAST dan analisis filogenetik. Jumlah bakteri di saluran pencernaan ikan nila dari tiga tempat relatif sama dan cenderung meningkat ke arah posterior. Hasil penelitian menunjukkan ikan nila dari Desa Pandak memiliki proporsi bakteri proteolitik yang lebih tinggi dibandingkan sampel ikan dari Desa Beji dan Tambaksogra. Nilai aktivitas bakteri proteolitik saluran pencernaan ikan nila dari Desa Pandak relatif lebih tinggi dibandingkan dari dua desa lainnya. Bakteri proteolitik dari saluran pencernaan ikan nila dapat dikelompokkan menjadi 15 kelompok berdasarkan polimorfisme hasil digesti fragment gen 16S rDNA. Sampel dari 15 kelompok tersebut memiliki sekuen 16S rDNA yang mirip dengan Pseudomonas aeruginosa (4 isolat), Plesiomonas shigelloides, Escherichia coli, Aeromonas veronii, Klebsiella variicola, Enterobacter ludwigii, Enterobacter hormaechei (2 isolat), Enterobacter cloacae, Bacillus subtilis, Bacillus amyloliquefaciens dan Bacillus sp.

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Controlling Pseudomonas aeruginosa infection in Oreochromis niloticus spawners by cefotaxime sodium

Cited by 21 publications

References 44 publications

Identification and florfenicol-treatment of pseudomonas putida infection in gilthead seabream (Sparus aurata) fed on tilapia-trash-feed

Identification and florfenicol-treatment of pseudomonas putida infection in gilthead seabream (Sparus aurata) fed on tilapia-trash-feed

The role of Aeromonas genotyping in virulence for Dicentrarchus labrax

Isolasi dan Identifikasi Molekuler Bakteri Proteolitik dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) yang Dibudidayakan di Kabupaten Banyumas

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