Controlling of two destructive zooplanktonic predators in Chlorella mass culture with surfactants

Wen, Xiaobin; Zhang, Aoqi; Zhu, Xiaoyan; Lin, Liang; Huo, Yan; Wang, Kaixuan; Yu, Youzhi; Geng, Yong; Ding, Yi; Li, Yeguang

doi:10.1186/s13068-021-01873-6

Cited by 7 publications

(2 citation statements)

References 40 publications

(58 reference statements)

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“…During the experiments, the maximum Fv/Fm (maximum photochemical yield) was measured using a PAM 2500 fluorometer, as described in [ 36 ]. ROS levels in G. emersonii cells were detected using an ROS assay kit (S0033S, Beyotime, China).…”

Section: Methodsmentioning

confidence: 99%

Physiological and Dual Transcriptional Analysis of Microalga Graesiella emersonii–Amoeboaphelidium protococcarum Pathosystem Uncovers Conserved Defense Response and Robust Pathogenicity

Ding

Wang

et al. 2021

IJMS

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The underlying mechanisms of microalgal host–pathogen interactions remain largely unknown. In this study, we applied physiological and simultaneous dual transcriptomic analysis to characterize the microalga Graesiella emersonii–Amoeboaphelidium protococcarum interaction. Three infection stages were determined according to infection rate and physiological features. Dual RNA-seq results showed that the genes expression of G. emersonii and A. protococcarum were strongly dynamically regulated during the infection. For microalgal hosts, similar to plant defense response, the expression of defense genes involved in the pattern recognition receptors, large heat shock proteins, and reactive oxygen scavenging enzymes (glutathione, ferritin, and catalase) were significantly upregulated during infection. However, some genes encoding resistance proteins (R proteins) with a leucine-rich repeat domain exhibited no significant changes during infection. For endoparasite A. protococcarum, genes for carbohydrate-active enzymes, pathogen–host interactions, and putative effectors were significantly upregulated during infection. Furthermore, the genes in cluster II were significantly enriched in pathways associated with the modulation of vacuole transport, including endocytosis, phagosome, ubiquitin-mediated proteolysis, and SNARE interactions in vesicular transport pathways. These results suggest that G. emersonii has a conserved defense system against pathogen and that endoparasite A. protococcarum possesses a robust pathogenicity to infect the host. Our study characterizes the first transcriptomic profile of microalgae–endoparasite interaction, providing a new promising basis for complete understanding of the algal host defense strategies and parasite pathogenicity.

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Section: Methodsmentioning

confidence: 99%

Physiological and Dual Transcriptional Analysis of Microalga Graesiella emersonii–Amoeboaphelidium protococcarum Pathosystem Uncovers Conserved Defense Response and Robust Pathogenicity

Ding

Wang

et al. 2021

IJMS

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“…Furthermore, it has been demonstrated well that the minimal effective concentration of sodium dodecyl benzene sulfonate (SDBS) required to completely eliminate Poterioochromonas sp. in Chlorella culture is 8 mg L −1 , and that the photosynthesis and viability of Chlorella is not significantly affected (Wen et al 2021). These results were validated in an outdoor raceway pond system with a maximum volume of 40,000 L. However, SDBS readily forms excessive bubbles in microalgal cultures and this leads to a decrease in microalgal biomass.…”

Section: Prevention and Control Methodsmentioning

confidence: 99%

A systematic review of the predatory contaminant Poterioochromonas in microalgal culture

Wei

Huang

et al. 2023

J Appl Phycol

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Contamination by zooplankton has to a certain extent limited the large-scale cultivation and industrial exploitation of microalgae. However, systematic research on these predators in microalgal culture is still lacking. The identification of zooplanktonic contaminants derived from microalgal cultures is a basis for conducting related studies. Moreover, knowledge of the ecological distribution of such predators is crucial for avoiding or reducing the risk of biological contamination in the management of large-scale microalgal cultures. Understanding the feeding behaviors of zooplanktonic contaminants contributes to the establishment of targeted prevention strategies and control methods. Early detection is essential to allow prevention and control measures to be implemented in a timely and effective way. Reducing the susceptibility of the cultured microalgae to predators through breeding strains selection, the potential of modern molecular methods, or a synthesis of these approaches will be indispensable to the management of zooplankton contamination. Furthermore, exploring the resource utilization of predators helps to understand this issue comprehensively and to turn hazard into wealth. The genus Poterioochromonas is a typical mixotrophic flagellate and has attracted increasing attention because of the dramatic damage it can inflict on a wide range of microalgal cultures, regardless of the culture system, season, or environment. This review explores our current understanding of the predator Poterioochromonas and the areas where further research is needed, which should stimulate reflection on what we still need to know about these predators from a microalgal culture perspective and how we can utilize them.

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Algal metabolites: Paving the way towards new generation antidiabetic therapeutics

et al. 2023

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Controlling of two destructive zooplanktonic predators in Chlorella mass culture with surfactants

Cited by 7 publications

References 40 publications

Physiological and Dual Transcriptional Analysis of Microalga Graesiella emersonii–Amoeboaphelidium protococcarum Pathosystem Uncovers Conserved Defense Response and Robust Pathogenicity

Physiological and Dual Transcriptional Analysis of Microalga Graesiella emersonii–Amoeboaphelidium protococcarum Pathosystem Uncovers Conserved Defense Response and Robust Pathogenicity

A systematic review of the predatory contaminant Poterioochromonas in microalgal culture

Algal metabolites: Paving the way towards new generation antidiabetic therapeutics

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