Controlling Lipid Membrane Architecture for Tunable Nanoplasmonic Biosensing

Zan, Goh Haw; Jackman, Joshua A.; Kim, Seong‐Oh; Cho, Nam‐Joon

doi:10.1002/smll.201400518

Cited by 44 publications

(62 citation statements)

References 28 publications

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“…The refractive index range explored was chosen to be identical to one that could be accessed experimentally using water/ethylene glycol solutions. [33][34] Figure 2c shows the Figure 2. Electric field intensity distribution around isolated nanodisks immersed in PBS obtained via FDTD modelling at the wavelength corresponding to the maximum of the LSPR.…”

Section: Resultsmentioning

confidence: 99%

“…35 Previous work by Cho and co-workers using biomolecules has reported a detailed study of the effect of dielectric coatings on interfacial chemistry and sensitivity. 34 In this work, the applicability of these nanostructured sensors to the study of protein adsorption at carbon surfaces in real time is demonstrated. Carbon coatings differ from typical oxide spacer layers, as their optical properties can vary significantly with electronic behavior that spans the semimetallic-semiconductor-insulator range.…”

Section: Introductionmentioning

confidence: 99%

“…To our knowledge NPS has not been used for the study of interactions at carbon coatings; herein we apply a recently reported NPS method developed by Kasemo et al based on Au nanodisk sensing elements. 32,[33][34] Studies of interfacial chemistry on this NPS platform have been typically carried using sensors coated with thin films of dielectrics, such as metal oxides or silica, which ensure a homogeneous surface chemistry and allow flexibility in terms of the chemical reactions under study. 35 Previous work by Cho and co-workers using biomolecules has reported a detailed study of the effect of dielectric coatings on interfacial chemistry and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanoplasmonic Sensing at the Carbon-Bio Interface: Study of Protein Adsorption at Graphitic and Hydrogenated Carbon Surfaces

Zen¹,

Karanikolas²,

Behan³

et al. 2017

Langmuir

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Various forms of carbon are known to perform well as biomaterials in a variety of applications and an improved understanding of their interactions with biomolecules, cells, and tissues is of interest for improving and tailoring their performance. Nanoplasmonic sensing (NPS) has emerged as a powerful technique for studying the thermodynamics and kinetics of interfacial reactions. In this work, the in situ adsorption of two proteins, bovine serum albumin and fibrinogen, were studied at carbon surfaces with differing chemical and optical properties using nanoplasmonic sensors. The carbon material was deposited as a thin film onto NPS surfaces consisting of 100 nm Au nanodisks with a localized plasmon absorption peak in the visible region. Carbon films were fully characterized by X-ray photoelectron spectroscopy, atomic force microscopy, and spectroscopic ellipsometry. Two types of material were investigated: amorphous carbon (a-C), with high graphitic content and high optical absorptivity, and hydrogenated amorphous carbon (a-C:H), with low graphitic content and high optical transparency. The optical response of the Au/carbon NPS elements was modeled using the finite difference time domain (FDTD) method, yielding simulated analytical sensitivities that compare well with those observed experimentally at the two carbon surfaces. Protein adsorption was investigated on a-C and a-C:H, and the protein layer thicknesses were obtained from FDTD simulations of the expected response, yielding values in the 1.8-3.3 nm range. A comparison of the results at a-C and a-C:H indicates that in both cases fibrinogen layers are thicker than those formed by albumin by up to 80%.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nanoplasmonic Sensing at the Carbon-Bio Interface: Study of Protein Adsorption at Graphitic and Hydrogenated Carbon Surfaces

Zen¹,

Karanikolas²,

Behan³

et al. 2017

Langmuir

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“…From a design perspective, the topic is particularly interesting because supported lipid membrane fabrication is challenging and a vast number of functionalization possibilities exist by selecting the appropriate membrane composition. Conventionally, supported lipid membranes are formed on silica-coated surfaces, which promote spontaneous bilayer formation whereas the formation process is less favorable on other materials such as gold and titanium oxide [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Ferhan

Junren

Jackman

et al. 2017

Sensors

Self Cite

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The integration of supported lipid membranes with surface-based nanoplasmonic arrays provides a powerful sensing approach to investigate biointerfacial phenomena at membrane interfaces. While a growing number of lipid vesicles, protein, and nucleic acid systems have been explored with nanoplasmonic sensors, there has been only very limited investigation of the interactions between solution-phase nanomaterials and supported lipid membranes. Herein, we established a surface-based localized surface plasmon resonance (LSPR) sensing platform for probing the interaction of dielectric nanoparticles with supported lipid bilayer (SLB)-coated, plasmonic nanodisk arrays. A key emphasis was placed on controlling membrane functionality by tuning the membrane surface charge vis-à-vis lipid composition. The optical sensing properties of the bare and SLB-coated sensor surfaces were quantitatively compared, and provided an experimental approach to evaluate nanoparticle–membrane interactions across different SLB platforms. While the interaction of negatively-charged silica nanoparticles (SiNPs) with a zwitterionic SLB resulted in monotonic adsorption, a stronger interaction with a positively-charged SLB resulted in adsorption and lipid transfer from the SLB to the SiNP surface, in turn influencing the LSPR measurement responses based on the changing spatial proximity of transferred lipids relative to the sensor surface. Precoating SiNPs with bovine serum albumin (BSA) suppressed lipid transfer, resulting in monotonic adsorption onto both zwitterionic and positively-charged SLBs. Collectively, our findings contribute a quantitative understanding of how supported lipid membrane coatings influence the sensing performance of nanoplasmonic arrays, and demonstrate how the high surface sensitivity of nanoplasmonic sensors is well-suited for detecting the complex interactions between nanoparticles and lipid membranes.

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“…Generally, the most often used surfaces in NPS applications include gold, SiO 2 , and TiO 2 surfaces. The interaction of the lipid membranes with the mentioned surfaces follows different adsorption pathways depending whether gold, SiO 2 , or TiO 2 coating is used (Zan et al, 2014). However, even usage of the same surface with different buffers can alter the adsorption pathway.…”

Section: Nps Analysis Of Aggregatesmentioning

confidence: 99%

Calcium Dependent Reversible Aggregation of Escherichia coli Biomimicking Vesicles Enables Formation of Supported Vesicle Layers on Silicon Dioxide

et al. 2019

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Controlling Lipid Membrane Architecture for Tunable Nanoplasmonic Biosensing

Cited by 44 publications

References 28 publications

Nanoplasmonic Sensing at the Carbon-Bio Interface: Study of Protein Adsorption at Graphitic and Hydrogenated Carbon Surfaces

Nanoplasmonic Sensing at the Carbon-Bio Interface: Study of Protein Adsorption at Graphitic and Hydrogenated Carbon Surfaces

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Calcium Dependent Reversible Aggregation of Escherichia coli Biomimicking Vesicles Enables Formation of Supported Vesicle Layers on Silicon Dioxide

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