Controlling insulin release from reverse hexagonal (HII) liquid crystalline mesophase by enzymatic lipolysis

Mishraki-Berkowitz, Tehila; Cohen, Guy; Aserin, Abraham; Garti, Nissim

doi:10.1016/j.colsurfb.2017.11.031

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…In previous studies, GMO cubic structures were used to protect insulin from aggregation induced by either agitation, temperature, or enzymatic degradation . On the other hand, different modified reverse hexagonal systems based on GMO and co-surfactants were used by Garti and co-workers to explore the effect of confinement on the stability, morphology, and unfolding behavior of the protein upon heating or pH changes. − …”

Section: Introductionmentioning

confidence: 99%

Cubic and Hexagonal Mesophases for Protein Encapsulation: Structural Effects of Insulin Confinement

et al. 2021

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Monoolein-based cubic and hexagonal mesophases were investigated as matrices for insulin loading, at low pH, as a function of temperature and in the presence of increasing amounts of oleic acid, as a structural stabilizer for the hexagonal phase. Synchrotron small angle X-ray diffraction, rheological measurements, and attenuated total reflection-Fourier transform infrared spectroscopy were used to study the effects of insulin loading on the lipid mesophases and of the effect of protein confinement in the 2D- and 3D-lipid matrix water channels on its stability and unfolding behavior. We found that insulin encapsulation has only little effects both on the mesophase structures and on the viscoelastic properties of lipid systems, whereas protein confinement affects the response of the secondary structure of insulin to thermal changes in a different manner according to the specific mesophase: in the cubic structure, the unfolding toward an unordered structure is favored, while the prevalence of parallel β-sheets, and nuclei for fibril formation, is observed in hexagonal structures.

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Section: Introductionmentioning

confidence: 99%

Cubic and Hexagonal Mesophases for Protein Encapsulation: Structural Effects of Insulin Confinement

et al. 2021

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“…LC systems have been shown to have high pharmaceutical value for their ability to deliver a range of molecules such as curcumin [ 13 ], chlorhexidine [ 14 ] and insulin [ 15 ]. LC systems encompass a number of phases such as lamellar phases, hexagonal phases and inverse bicontinuous cubic phases.…”

Section: Introductionmentioning

confidence: 99%

Novel self-assembling conjugates as vectors for agrochemical delivery

et al. 2018

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BackgroundModern agricultural practises rely on surfactant-based spray applications to eliminate weeds in crops. The wide spread and indiscriminate use of surfactants may result in a number of deleterious effects that are not limited to impacts on the crop and surrounding farm eco-system but include effects on human health. To provide a safer alternative to the use of surfactant-based formulations, we have synthesised a novel, self-assembling herbicide conjugate for the delivery of a broad leaf herbicide, picloram.ResultsThe synthesized self-assembling amphiphile–picloram (SAP) conjugate has three extending arms: a lipophilic lauryl chain, a hydrophilic polyethylene glycol chain and the amphiphobic agrochemical active picloram. We propose that the SAP conjugate maintains its colloidal stability by quickly transitioning between micellar and inverse micellar phases in hydrophilic and lipophilic environments respectively. The SAP conjugate provides the advantage of a phase structure that enables enhanced interaction with the hydrophobic epicuticular wax surface of the leaf. We have investigated the herbicidal efficiency of the SAP conjugate compared against that of commercial picloram formulations using the model plant Arabidopsis thaliana and found that when tested at agriculturally relevant doses between 0.58 and 11.70 mM a dose-dependent herbicidal effect with comparable kill rates was evident.ConclusionThough self-assembling drug carriers are not new to the pharmaceutical industry their use for the delivery of agrochemicals shows great promise but is largely unexplored. We have shown that SAP may be used as an alternative to current surfactant-based agrochemical formulations and has the potential to shift present practises towards a more sustainable approach.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0423-5) contains supplementary material, which is available to authorized users.

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Phase transition and release kinetics of polyphenols encapsulated lyotropic liquid crystals

Zhang

Wang

2019

International Journal of Pharmaceutics

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Controlling insulin release from reverse hexagonal (HII) liquid crystalline mesophase by enzymatic lipolysis

Cited by 4 publications

References 45 publications

Cubic and Hexagonal Mesophases for Protein Encapsulation: Structural Effects of Insulin Confinement

Cubic and Hexagonal Mesophases for Protein Encapsulation: Structural Effects of Insulin Confinement

Novel self-assembling conjugates as vectors for agrochemical delivery

Phase transition and release kinetics of polyphenols encapsulated lyotropic liquid crystals

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