Controlling Growth Factor Diffusion by Modulating Water Content in Injectable Hydrogels

Dromel, Pierre C.; Singh, Deepti; Christoff‐Tempesta, Ty; Martheswaran, Tanisha; Alexander‐Katz, Alfredo; Spector, Myron; Young, Michael

doi:10.1089/ten.tea.2020.0313

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…Being able to attach our in situ-crosslinking hydrogel to the back of the eye (ILM) is one of the core results of our study underscoring its ability to enhance not only cell therapies but also vitreal drug injections 32 .…”

Section: Resultsmentioning

confidence: 86%

A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy

et al. 2021

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Biomaterial-based cell replacement approaches to regenerative medicine are emerging as promising treatments for a wide array of profound clinical problems. Here we report an interpenetrating polymer network (IPN) composed of gelatin-hydroxyphenyl propionic acid and hyaluronic acid tyramine that is able to enhance intravitreal retinal cell therapy. By tuning our bioinspired hydrogel to mimic the vitreous chemical composition and mechanical characteristics we were able to improve in vitro and in vivo viability of human retinal ganglion cells (hRGC) incorporated into the IPN. In vivo vitreal injections of cell-bearing IPN in rats showed extensive attachment to the inner limiting membrane of the retina, improving with hydrogels stiffness. Engrafted hRGC displayed signs of regenerating processes along the optic nerve. Of note was the decrease in the immune cell response to hRGC delivered in the gel. The findings compel further translation of the gelatin-hyaluronic acid IPN for intravitreal cell therapy.

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Section: Resultsmentioning

confidence: 86%

A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy

et al. 2021

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“…Hydrogel properties also influence release properties along with cell behavior. On a separate study from the same group, the diffusion and release of human epidermal growth factors (hEGF) decrease with stiffness of Gtn‐HPA and HA‐Tyr hydrogels 41 . We direct the readers to recent reviews by Sakpal et.…”

Section: Ophthalmologymentioning

confidence: 99%

“…decrease with stiffness of Gtn-HPA and HA-Tyr hydrogels. 41 We direct the readers to recent reviews by Sakpal et. al and Rizzolo et al…”

Section: Retinamentioning

confidence: 99%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

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“…Therein, the uptake is primarily independent of the physical environment except potentially by diffusion coefficients in 3D matrixes such as hydrogels. [111,112] All these layers of complexity within the current knowledge on BMPs in bone TE leave plenty of room to find the perfect BMP/biomaterial combination for different applications. This case is further strengthened because whole BMPs have also been replaced by smaller peptides derived from the entire sequence.…”

Section: Growth-factor-based Approachesmentioning

confidence: 99%

Pushing the Natural Frontier: Progress on the Integration of Biomaterial Cues toward Combinatorial Biofabrication and Tissue Engineering

2022

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and shown able to integrate the organism and maintain physiological function for many years after implantation. Even though these are examples of success, they represent mostly nonvital organs generally simpler in terms of morphology and cellular complexity. On the other hand, vital organs like the kidney, heart, liver, or lungs top the list of transplant requirements worldwide. [4] Yet, there are no TE equivalents of these organs so far, resulting from their complex cellular function and architecture, which are extremely hard to engineer, leading to a continuous effort to better organ recovery and transplantation. [5] Biomaterials have been faithful companions of cells in TE strategies, physically supporting them and allowing the maintenance of their phenotype in distinct environments. In fact, with the recent advances in the field of 3D printing, biomaterial-based 3D structures can now be manipulated to approach the architecture of complex tissues and organs, such as vascular beds and cardiac components. [6,7] However, shape and architecture alone cannot assure the ultimate TE challenge: recapitulation of physiological cellular and tissue function. As such, there is one additional burden for biomaterials to carry-the control of cellular behavior. These requirements force upon biomaterials the need to be intelligent and dynamic, supporting and efficiently directing the behavior of cells toward specific outcomes.Natural-origin materials have been continuously derived from different animal and plant components, gathering attention as biomaterials due to their original role in biological environments. [8] However, their bioactivity and biodegradability can be just as limited as that of synthetic components. Thus, this review explores some of the leading natural materials that have been used in TE approaches, looking at their typical applications, degradability properties, and ability to interact with and be modified by the action of cells-a biomimetic, extracellular matrix (ECM)-like behavior. Furthermore, we explore the progress on the use of adhesive sequences and growth factors and their combinatorial applications with emerging synergistic results and novel biological outcomes. Likewise, as force and shape establish themselves as fair opponents to classical biochemical cues, [9,10] we review the recent progress in manipulating stiffness and topography within 3D natural materials and how they can further boost cellular responses.The engineering of fully functional, biological-like tissues requires biomaterials to direct cellular events to a near-native, 3D niche extent. Natural biomaterials are generally seen as a safe option for cell support, but their biocompatibility and biodegradability can be just as limited as their bioactive/ biomimetic performance. Furthermore, integrating different biomaterial cues and their final impact on cellular behavior is a complex equation where the outcome might be very different from the sum of individual parts. This review critically analyses recent progress on biomaterial-indu...

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Controlling Growth Factor Diffusion by Modulating Water Content in Injectable Hydrogels

Cited by 8 publications

References 32 publications

A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy

A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Pushing the Natural Frontier: Progress on the Integration of Biomaterial Cues toward Combinatorial Biofabrication and Tissue Engineering

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